CoChat
Sign Up
Cited by 426Microtubule and mitosis dynamicsProtein Kinase Regulation and GTPase SignalingCellular Mechanics and Interactions

The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.

Anillin Is a Scaffold Protein That Links RhoA, Actin, and Myosin during Cytokinesis

Open access
Piekny, Alisa J., Glotzer, Michael·2008·Current Biology
DOI 10.1016/j.cub.2007.11.068
Open this paper in CoChat — free accountOpen PDF

Abstract

Cell division after mitosis is mediated by ingression of an actomyosin-based contractile ring. The active, GTP-bound form of the small GTPase RhoA is a key regulator of contractile-ring formation. RhoA concentrates at the equatorial cell cortex at the site of the nascent cleavage furrow. During cytokinesis, RhoA is activated by its RhoGEF, ECT2. Once activated, RhoA promotes nucleation, elongation, and sliding of actin filaments through the coordinated activation of both formin proteins and myosin II motors (reviewed in [1, 2]). Anillin is a 124 kDa protein that is highly concentrated in the cleavage furrow in numerous animal cells in a pattern that resembles that of RhoA [3-7]. Although anillin contains conserved N-terminal actin and myosin binding domains and a PH domain at the C terminus, its mechanism of action during cytokinesis remains unclear. Here, we show that human anillin contains a conserved C-terminal domain that is essential for its function and localization. This domain shares homology with the RhoA binding protein Rhotekin and directly interacts with RhoA. Further, anillin is required to maintain active myosin in the equatorial plane during cytokinesis, suggesting it functions as a scaffold protein to link RhoA with the ring components actin and myosin. Although furrows can form and initiate ingression in the absence of anillin, furrows cannot form in anillin-depleted cells in which the central spindle is also disrupted, revealing that anillin can also act at an early stage of cytokinesis.

Overview

Integrity signals

Registry

Crossref · 10.1016/j.cub.2007.11.068

Cites

28 of 28 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Flagged first, then by citations

Show all 28 references

Cited by

Sorted by citations

Items 401–415 of 415

Related

See all

Metadata

Authors
Piekny, A. J., & Glotzer, M.
Affiliations
University of Chicago (US)
Corresponding author
Glotzer, Michael
ORCID
Venue
Current Biology
Publisher
Elsevier BV
Published
2008-01
Volume
18
Issue
1
Pages
30-36
ISSN
0960-9822 (ISSN-L)
DOI
10.1016/j.cub.2007.11.068
OpenAlex
W2049863303
Cited by
426
References
28
Citations by year
2026: 8 · 2025: 12 · 2024: 20 · 2023: 29 · 2022: 34 · 2021: 30 · 2020: 37 · 2019: 26 · 2018: 24 · 2017: 29 · 2016: 33 · 2015: 26 · 2014: 19 · 2013: 18 · 2012: 16
FWCI
9.48
Type
journal article
Language
en
Open access
Licence
Locations
  • Current Biology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Canadian Institutes of Health Research
  • National Institutes of Health (GM074743-01)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
Keywords
anillin, cytokinesis, RhoA, contractile ring, cleavage furrow, myosin II, actomyosin, scaffold proteins, Rhotekin, central spindle, actin, formins, cell division
MeSH terms
Actins, Amino Acid Sequence, Contractile Proteins, HeLa Cells, Humans, Spindle Apparatus, Molecular Sequence Data, Myosins, Sequence Alignment, Conserved Sequence, Protein Structure, Tertiary, GTP-Binding Proteins, rhoA GTP-Binding Protein, Intracellular Signaling Peptides and Proteins, Cytokinesis, Apoptosis Regulatory Proteins

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review