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Cited by 154

Argonaute Loading Improves the 5′ Precision of Both MicroRNAs and Their miRNA∗ Strands in Flies

Seitz, Hervé, Ghildiyal, Megha, Zamore, Phillip D.·2008·Current Biology
DOI 10.1016/j.cub.2007.12.049
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Crossref · 10.1016/j.cub.2007.12.049

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  • Drosha and Dicer appear to generate 5′ and 3′ isomiRs at similar frequencies.

    “Indeed, Drosha and Dicer appear to generate 5′ and 3′ isomiRs at similar frequencies (Seitz et al., 2008)”

    From microRNA strand selection: Unwinding the rules (2021) · cites this paper as Seitz et al., 2008

    The Results and Discussion proposes that Drosha and Dicer may generate 5′ and 3′ ends with similar, imperfect accuracy, and reports that Ago2 loading increases the apparent precision of 5′ ends. It does not establish that the enzymes generate 5′ and 3′ isomiRs at similar frequencies; that remains a proposed explanation rather than a measured frequency comparison. (LLM, conf=0.78)

    Experimental — accuracy not yet measured

  • For the majority of miRNAs, both strands have precisely defined 5' termini compared to their 3' ends, suggesting evolutionary pressure to maintain the target-determining sequence of both strands.

    “In addition, for majority of miRNAs, both strands have precisely defined 5′ termini, compared to their 3′ ends, suggesting evolutionary pressure to maintain the target‐determining sequence of both strands (Okamura et al., 2008; Ruby, Jan, et al., 2007; Ruby, Stark, et al., 2007; Seitz, Ghildiyal, & Zamore, 2008).”

    From microRNA strand selection: Unwinding the rules (2021) · cites this paper as Seitz, Ghildiyal, & Zamore, 2008

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Research works printed but not linked: 20

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Cited by

Sorted by citations

Authors
Seitz, H., Ghildiyal, M., & Zamore, P. D.
Venue
Current Biology
Publisher
Elsevier BV
Published
2008-01
Volume
18
Issue
2
Pages
147-151
ISSN
0960-9822
DOI
10.1016/j.cub.2007.12.049
Cited by
154
References
1
Language
en
Licence
Funders
  • European Molecular Biology Organization (ALTF 910-2004)
  • Alnylam Pharmaceuticals
  • Human Frontier Science Program (LT00575/2005-L)
  • National Institutes of Health (GM62862, GM65236)

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