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Neutrophil-Delivered Myeloperoxidase Dampens the Hydrogen Peroxide Burst after Tissue Wounding in Zebrafish
Open accessAbstract
Prompt neutrophil arrival is critical for host defense immediately after injury [1-3]. Following wounding, a hydrogen peroxide (H(2)O(2)) burst generated in injured tissues is the earliest known leukocyte chemoattractant [4]. Generating this tissue-scale H(2)O(2) gradient uses dual oxidase [4] and neutrophils sense H(2)O(2) by a mechanism involving the LYN Src-family kinase [5], but the molecular mechanisms responsible for H(2)O(2) clearance are unknown [6]. Neutrophils carry abundant amounts of myeloperoxidase, an enzyme catalyzing an H(2)O(2)-consuming reaction [7, 8]. We hypothesized that this neutrophil-delivered myeloperoxidase downregulates the high tissue H(2)O(2) concentrations that follow wounding. This was tested in zebrafish using simultaneous fluorophore-based imaging of H(2)O(2) concentrations and leukocytes [4, 9-11] and a new neutrophil-replete but myeloperoxidase-deficient mutant (durif). Leukocyte-depleted zebrafish had an abnormally sustained wound H(2)O(2) burst, indicating that leukocytes themselves were required for H(2)O(2) downregulation. Myeloperoxidase-deficient zebrafish also had abnormally sustained high wound H(2)O(2) concentrations despite similar numbers of arriving neutrophils. A local H(2)O(2)/myeloperoxidase interaction within wound-recruited neutrophils was demonstrated. These data demonstrate that leukocyte-delivered myeloperoxidase cell-autonomously downregulates tissue-generated wound H(2)O(2) gradients in vivo, defining a new requirement for myeloperoxidase during inflammation. Durif provides a new animal model of myeloperoxidase deficiency closely phenocopying the prevalent human disorder [7, 12, 13], offering unique possibilities for investigating its clinical consequences.
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“For example, neutrophils may be partly responsible for their own resolution by clearance of the attractants that first drew them to the wound (128).”
From Wound repair and regeneration: Mechanisms, signaling, and translation (2014) · cites this paper as [128]
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Cites
Neutrophils in the activation and regulation of innate and adaptive immunity
Mantovani, A., Cassatella, M. A., Costantini, C., & Jaillon, S. (2011) · Nature reviews. Immunology · doi:10.1038/nri3024 · Cited by 3,159
Myeloperoxidase: friend and foe
Klebanoff, S. J. (2005) · Journal of Leukocyte Biology · doi:10.1189/jlb.1204697 · Cited by 2,282
Segal, A. W. (2005) · Annual Review of Immunology · doi:10.1146/annurev.immunol.23.021704.115653 · Cited by 1,783
Neutrophils, from Marrow to Microbes
Borregaard, N. (2010) · Immunity · doi:10.1016/j.immuni.2010.11.011 · Cited by 1,679
A tissue-scale gradient of hydrogen peroxide mediates rapid wound detection in zebrafish
Niethammer, P., Grabher, C., Look, A. T., & Mitchison, T. J. (2009) · Nature · doi:10.1038/nature08119 · Cited by 1,541
Self-citationGenetically encoded fluorescent indicator for intracellular hydrogen peroxide
Belousov, V. V., Fradkov, A. F., Lukyanov, K. A., Staroverov, D. B., Shakhbazov, K., Terskikh, A. V., & Lukyanov, S. (2006) · Nature Methods · doi:10.1038/nmeth866 · Cited by 1,286
mpeg1 promoter transgenes direct macrophage-lineage expression in zebrafish
Ellett, F. E., Pase, L. B., Hayman, J. W., Andrianopoulos, A., & Lieschke, G. J. (2010) · Blood · doi:10.1182/blood-2010-10-314120 · Cited by 1,085
Self-citationA transgenic zebrafish model of neutrophilic inflammation
Renshaw, S. A., Loynes, C. A., Trushell, D. M. I., Elworthy, S., Ingham, P. W., & Whyte, M. K. B. (2006) · Blood · doi:10.1182/blood-2006-05-024075 · Cited by 1,013
Self-citationLehrer, R. I., & Cline, M. J. (1969) · Journal of Clinical Investigation · doi:10.1172/jci106114 · Cited by 688
The zebrafish lysozyme C promoter drives myeloid-specific expression in transgenic fish
Hall, C. J., Flores, M. V., Storm, T., Crosier, K., & Crosier, P. S. (2007) · BMC Developmental Biology · doi:10.1186/1471-213x-7-42 · Cited by 589
Self-citationResidual NADPH Oxidase and Survival in Chronic Granulomatous Disease
Kuhns, D. B., Alvord, W. G., Heller, T., Feld, J. J., Pike, K. M., Marciano, B. E., Uzel, G., DeRavin, S. S., Priel, D. A. L., Soule, B. P., et al. (2010) · New England Journal of Medicine · doi:10.1056/nejmoa1007097 · Cited by 568
Myeloperoxidase: Molecular Mechanisms of Action and Their Relevance to Human Health and Disease
van der Veen, B. S., de Winther, M. P. J., & Heeringa, P. (2009) · Antioxidants and Redox Signaling · doi:10.1089/ars.2009.2538 · Cited by 530
Stainier, D. Y. R., Weinstein, B. M., Detrich, H. W., Zon, L. I., & Fishman, M. C. (1995) · Development · doi:10.1242/dev.121.10.3141 · Cited by 522
Lieschke, G. J., Oates, A. C., Crowhurst, M. O., Ward, A. C., & Layton, J. E. (2001) · Blood · doi:10.1182/blood.v98.10.3087 · Cited by 489
Self-citationLyn is a redox sensor that mediates leukocyte wound attraction in vivo
Yoo, S. K., Starnes, T. W., Deng, Q., & Anna Huttenlocher, A. (2011) · Nature · doi:10.1038/nature10632 · Cited by 442
Myeloperoxidase acts as a profibrotic mediator of atrial fibrillation
Rudolph, V., Andrié, R., Rudolph, T. K., Friedrichs, K. P., Klinke, A., Hirsch-Hoffmann, B., Schwoerer, A. P., Lau, D., Fu, X., Klingel, K., et al. (2010) · Nature Medicine · doi:10.1038/nm.2124 · Cited by 352
Severe Impairment in Early Host Defense againstCandida albicans in Mice Deficient in Myeloperoxidase
Aratani, Y., Koyama, H., Nyui, S.-I., Suzuki, K. T., Kura, F., & Maeda, N. (1999) · Infection and Immunity · doi:10.1128/iai.67.4.1828-1836.1999 · Cited by 322
Increased atherosclerosis in myeloperoxidase-deficient mice
Brennan, M., Anderson, M. M., Shih, D. M., Qu, X.-D., Wang, X., Mehta, A., Lim, L. L., Shi, W., Hazen, S. L., Jacob, J. S., et al. (2001) · Journal of Clinical Investigation · doi:10.1172/jci8797 · Cited by 321
Human myeloperoxidase in innate and acquired immunity
Arnhold, J., & Flemmig, J. (2010) · Archives of Biochemistry and Biophysics · doi:10.1016/j.abb.2010.04.008 · Cited by 254
Consequences of Total and Subtotal Myeloperoxidase Deficiency: Risk or Benefit ?
Kutter, D., Devaquet, P., Vanderstocken, G., Paulus, J., Marchal, V., & Gothot, A. (2000) · Acta Haematologica · doi:10.1159/000041062 · Cited by 250
ZFIN: enhancements and updates to the zebrafish model organism database
Bradford, Y. M., Conlin, T., Dunn, N., Fashena, D. S., Frazer, K., Howe, D. G., Knight, J., Mani, P., Martin, R., Moxon, S. A. T., et al. (2010) · Nucleic Acids Research · doi:10.1093/nar/gkq1077 · Cited by 230
Feng, Y., Santoriello, C., Mione, M., Hurlstone, A. F. L., & Martin, P. B. (2010) · PLoS Biology · doi:10.1371/journal.pbio.1000562 · Cited by 207
Moreira, S., Stramer, B. M., Evans, I. R., Wood, W., & Martin, P. B. (2010) · Current Biology · doi:10.1016/j.cub.2010.01.047 · Cited by 194
Myeloperoxidase attracts neutrophils by physical forces
Klinke, A., Nußbaum, C., Kubala, L., Friedrichs, K. P., Rudolph, T. K., Rudolph, V., Paust, H., Schröder, C., Benten, D., Lau, D., et al. (2010) · Blood · doi:10.1182/blood-2010-05-284513 · Cited by 184
Hydrogen Peroxide Promotes Injury-Induced Peripheral Sensory Axon Regeneration in the Zebrafish Skin
Rieger, S., & Sagasti, A. (2011) · PLoS Biology · doi:10.1371/journal.pbio.1000621 · Cited by 177
Hydrogen peroxide-induced chemotaxis of mouse peritoneal neutrophils.
Klyubin, I. V., Kirpichnikova, K. M., & Gamaley, I. A. (1996) · PubMed · Cited by 137
Meijer, A. H., van der Sar, A. M., da Cunha, C. B., Lamers, G. E. M., Laplante, M. A., Kikuta, H., Bitter, W., Becker, T. S., & Spaink, H. P. (2007) · Developmental & Comparative Immunology · doi:10.1016/j.dci.2007.04.003 · Cited by 131
Nauseef, W. M. (1988) · Hematology/Oncology Clinics of North America · doi:10.1016/s0889-8588(18)30634-8 · Cited by 103
Specification of the Primitive Myeloid Precursor Pool Requires Signaling through Alk8 in Zebrafish
Hogan, B. M., Layton, J. E., Pyati, U. J., Nutt, S. L., Hayman, J. W., Varma, S., Heath, J. K., Kimelman, D., & Lieschke, G. J. (2006) · Current Biology · doi:10.1016/j.cub.2006.01.047 · Cited by 49
Self-citationInnate Immunity: Wounds Burst H2O2 Signals to Leukocytes
Yoo, S. K., & Huttenlocher, A. (2009) · Current Biology · doi:10.1016/j.cub.2009.06.025 · Cited by 42
Breaking ranks: how leukocytes react to developmental cues and tissue injury
Peri, F. (2010) · Current Opinion in Genetics & Development · doi:10.1016/j.gde.2010.05.002 · Cited by 7
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Eming, S. A., Martin, P., & Tomic-Canic, M. (2014) · Science Translational Medicine · doi:10.1126/scitranslmed.3009337 · Cited by 3,562
Inflammation and metabolism in tissue repair and regeneration
Eming, S. A., Wynn, T. A., & Martin, P. (2017) · Science · doi:10.1126/science.aam7928 · Cited by 1,369
Cellular and molecular mechanisms of repair in acute and chronic wound healing
Martin, P. B., & Nunan, R. D. (2015) · British Journal of Dermatology · doi:10.1111/bjd.13954 · Cited by 1,183
Neutrophil migration in infection and wound repair: going forward in reverse
de Oliveira, S., Rosowski, E. E., & Huttenlocher, A. (2016) · Nature Reviews Immunology · doi:10.1038/nri.2016.49 · Cited by 1,058
Preparing the ground for tissue regeneration: from mechanism to therapy
Forbes, S. J., & Rosenthal, N. A. (2014) · Nature Medicine · doi:10.1038/nm.3653 · Cited by 607
Metadata
- Authors
- Pase, L., Layton, J. E., Wittmann, C., Ellett, F., Nowell, C. J., Reyes-Aldasoro, C. C., Varma, S., Rogers, K. L., Hall, C. J., Keightley, M. C., Crosier, P. S., Grabher, C., Heath, J. K., Renshaw, S. A., & Lieschke, G. J.
- Affiliations
- The University of Melbourne (AU)
- Walter and Eliza Hall Institute of Medical Research (AU)
- Karlsruhe Institute of Technology (DE)
- Ludwig Cancer Research (BE)
- Australian Regenerative Medicine Institute (AU)
- University of Auckland (NZ)
- University of Sheffield (GB)
- Corresponding author
- Lieschke, Graham J.
- ORCID
- Venue
- Current Biology
- Publisher
- Elsevier BV
- Published
- 2012-10
- Volume
- 22
- Issue
- 19
- Pages
- 1818-1824
- ISSN
- 0960-9822 (ISSN-L)
- OpenAlex
- W2171239786
- Cited by
- 118
- References
- 32
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 4 · 2023: 8 · 2022: 3 · 2021: 10 · 2020: 13 · 2019: 11 · 2018: 13 · 2017: 5 · 2016: 7 · 2015: 12 · 2014: 16 · 2013: 8 · 2012: 1
- FWCI
- 5.15
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Current Biology (publishedVersion)
- PubMed (publishedVersion)
- City Research Online (City University London) (acceptedVersion)
- Figshare (submittedVersion, cc-by)
- ResearchSpace (University of Auckland) (submittedVersion)
- Funders
- National Heart, Lung, and Blood Institute (R01HL079545)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Health Sciences › Medicine › Surgery › Wound Healing and Treatments
- Keywords
- myeloperoxidase, neutrophils, hydrogen peroxide, wound healing, tissue damage, dual oxidase, Lyn kinase, myeloperoxidase deficiency, inflammation, leukocyte recruitment, zebrafish
- MeSH terms
- Animals, Hydrogen Peroxide, Leukocytes, Mutation, Peroxidase, Neutrophils, Zebrafish, Neutrophil Infiltration, Animals, Genetically Modified
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