Evolution: Two Domains of Life or Three?
Open accessAbstract
That Bacteria, Archaea and Eukarya (eukaryotes) represent three separate domains of Life, no one having evolved from within any other, has been taken as fact for three decades. Recent work shows this to be untrue. Eukarya arose from well within Archaea and are specifically related to newly discovered archaeal species with eukaryote-like features.
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Towards a natural system of organisms: proposal for the domains Archaea, Bacteria, and Eucarya.
Woese, C. R., Kandler, O., & Wheelis, M. L. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.12.4576 · Cited by 6,128 · see citing works
Complex archaea that bridge the gap between prokaryotes and eukaryotes
Spang, A., Saw, J. H., Jørgensen, S. L., Zaremba-Niedzwiedzka, K., Martijn, J., Lind, A. E., van Eijk, R., Schleper, C., Guy, L., & Ettema, T. J. G. (2015) · Nature · doi:10.1038/nature14447 · Cited by 1,324 · see citing works
Asgard archaea illuminate the origin of eukaryotic cellular complexity
Zaremba-Niedzwiedzka, K., Caceres, E. F., Saw, J. H., Bäckström, D., Juzokaite, L., Vancaester, E., Seitz, K. W., Anantharaman, K., Starnawski, P., Kjeldsen, K. U., et al. (2017) · Nature · doi:10.1038/nature21031 · Cited by 1,226 · see citing works
Woese, C. R. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.132266999 · Cited by 762 · see citing works
Isolation of an archaeon at the prokaryote–eukaryote interface
Imachi, H., Nobu, M. K., Nakahara, N., Morono, Y., Ogawara, M., Takaki, Y., Takano, Y., Uematsu, K., Ikuta, T., Ito, M., et al. (2020) · Nature · doi:10.1038/s41586-019-1916-6 · Cited by 624 · see citing works
An archaeal origin of eukaryotes supports only two primary domains of life
Williams, T. A., Foster, P. G., Cox, C. J., & Embley, T. M. (2013) · Nature · doi:10.1038/nature12779 · Cited by 519 · see citing works
The concept of cellular evolution
Woese, C. R., & Fox, G. E. (1977) · Journal of Molecular Evolution · doi:10.1007/bf01796132 · Cited by 388 · see citing works
Evidence that eukaryotes and eocyte prokaryotes are immediate relatives
Rivera, M. C., & Lake, J. A. (1992) · Science · doi:10.1126/science.1621096 · Cited by 342 · see citing works
Implications of RNA-RNA splicing in evolution of eukaryotic cells.
Darnell, J. (1978) · Science · doi:10.1126/science.364651 · Cited by 299 · see citing works
Phylogenomics provides robust support for a two-domains tree of life
Williams, T. A., Cox, C. J., Foster, P. G., Szöllősi, G. J., & Embley, T. M. (2019) · Nature Ecology & Evolution · doi:10.1038/s41559-019-1040-x · Cited by 274 · see citing works
Mayr, E. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.17.9720 · Cited by 183 · see citing works
Default taxonomy: Ernst Mayr’s view of the microbial world
Woese, C. R. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.19.11043 · Cited by 155 · see citing works
The primary divisions of life: a phylogenomic approach employing composition-heterogeneous methods
Foster, P. G., Cox, C. J., & Embley, T. M. (2009) · Philosophical Transactions of the Royal Society B Biological Sciences · doi:10.1098/rstb.2009.0034 · Cited by 120 · see citing works
Eukaryogenesis, how special really?
Booth, A., & Doolittle, W. F. (2015) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1421376112 · Cited by 116 · see citing works
Self-citationTowards functional characterization of archaeal genomic dark matter
Makarova, K. S., Wolf, Y. I., & Koonin, E. V. (2019) · Biochemical Society Transactions · doi:10.1042/bst20180560 · Cited by 51 · see citing works
Farming the mitochondrial ancestor as a model of endosymbiotic establishment by natural selection
Zachar, I., Szilágyi, A., Számadó, S., & Szathmáry, E. (2018) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1718707115 · Cited by 39 · see citing works
Symbiogenesis, gradualism, and mitochondrial energy in eukaryote origin
Martin, W. F. (2017) · Periodicum Biologorum · doi:10.18054/pb.v119i3.5694 · Cited by 38 · see citing works
Eukaryotes first: how could that be?
Mariscal, C., & Doolittle, W. F. (2015) · Philosophical Transactions of the Royal Society B Biological Sciences · doi:10.1098/rstb.2014.0322 · Cited by 31 · see citing works
Self-citationChanging ideas about eukaryotic origins
Williams, T. A., & Embley, T. M. (2015) · Philosophical Transactions of the Royal Society B Biological Sciences · doi:10.1098/rstb.2014.0318 · Cited by 26 · see citing works
Flagged first, then by citations
Research works printed but not linked: 1
- Stanier, 1970, Some aspects of the biology of cells and their possible evolutionary significance, Symp. Soc. Gen. Microbiol., 20, 1
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Boomsma, J. J. (2022) · doi:10.1093/oso/9780198746171.001.0001 · Cited by 54 · see citing works
Supersized Ribosomal RNA Expansion Segments in Asgard Archaea
Penev, P. I., Fakhretaha-Aval, S., Patel, V. J., Cannone, J. J., Gutell, R. R., Petrov, A. S., Williams, L. D., & Glass, J. B. (2020) · Genome Biology and Evolution · doi:10.1093/gbe/evaa170 · Cited by 35 · see citing works
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“The true evolutionary position of eukaryotes is hence the subject of continuing debates and it has still not been widely agreed if eukaryotes represent a separate domain (Williams et al., 2013; Doolittle, 2020).”
BackgroundŘezanka, T., Kyselová, L., & Murphy, D. J. (2023) · Progress in Lipid Research · doi:10.1016/j.plipres.2023.101237 · Cited by 33 · see citing works
RetractedInferring the Deep Past from Molecular Data
Williams, T., Schrempf, D., Szöllősi, G., Cox, C., Foster, P., & Embley, T. (2021) · Genome Biology and Evolution · doi:10.1093/gbe/evab067 · Cited by 27 · see citing works
“Doolittle WF. 2020.”
Role of aIF5B in archaeal translation initiation
Kazan, R., Bourgeois, G., Lazennec‐Schurdevin, C., Larquet, É., Méchulam, Y., Coureux, P., & Schmitt, E. (2022) · Nucleic Acids Research · doi:10.1093/nar/gkac490 · Cited by 21 · see citing works
The Role of Gene Duplication in the Divergence of Enzyme Function: A Comparative Approach
Álvarez-Lugo, A., & Becerra, A. (2021) · Frontiers in Genetics · doi:10.3389/fgene.2021.641817 · Cited by 16 · see citing works
“…view of life, which suggests that eukaryotes do not constitute a separate domain but are part of the Archaea domain (Williams et al., 2013; Doolittle, 2020), we decided only to consider prokaryotic organisms, which as a group possess a much wider biochemical repertoire than that for…”
BackgroundLoh, D., & Reiter, R. J. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23158122 · Cited by 17 · see citing works
Siebieszuk, A., Sejbuk, M., & Witkowska, A. M. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms242216169 · Cited by 12 · see citing works
“Although the debate on this matter remains intense [119,129–133], advances in phylogenomic, These are known as methano-gens and represent the most dominant archaea in the human gut.”
BackgroundRenewing L innaean taxonomy: a proposal to restructure the highest levels of the N atural S ystem
van der Gulik, P. T. S., Hoff, W. D., & Speijer, D. (2022) · Biological reviews/Biological reviews of the Cambridge Philosophical Society · doi:10.1111/brv.12920 · Cited by 11 · see citing works
“Such cladistic purity has resulted in calls for the domain Eukaryotes to be abandoned (Williams et al., 2013; Raymann, BrochierArmanet & Gribaldo, 2015; Doolittle, 2020; Williams et al., 2020).”
BackgroundProtists with Uncertain Phylogenetic Affiliations for Resolving the Deep Tree of Eukaryotes
Yazaki, E., Shiratori, T., & Inagaki, Y. (2025) · Microorganisms · doi:10.3390/microorganisms13081926 · Cited by 5 · see citing works
The Origin of Life and Cellular Systems: A Continuum from Prebiotic Chemistry to Biodiversity
Gómez‐Márquez, J. (2025) · Life · doi:10.3390/life15111745 · Cited by 9 · see citing works
“More recent models, such as the two-domain hypothesis, propose that eukaryotes emerged from within the archaeal domain, yet still place LUCA at the root of the evolutionary tree [157].”
Tomajoli, M. T. M., Donato, P. D., Corte, V. D., Covone, G., Fasciolo, G., Geremia, E., Petito, A., Tonietti, L., Inno, L., Rotundi, A., et al. (2025) · Comparative Biochemistry and Physiology Part A Molecular & Integrative Physiology · doi:10.1016/j.cbpa.2025.111825 · Cited by 5 · see citing works
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Chin, A. F., Wrabl, J. O., & Hilser, V. J. (2022) · Molecular Biology and Evolution · doi:10.1093/molbev/msac010 · Cited by 4 · see citing works
“State-of-the-art phylogenetic trees, built from primary sequence relationships, consistently reveal that eukaryotes are more closely related to archaea rather than bacteria, probably through transitional Asgard archaea (Eme et al. 2018; Doolittle 2020; Liu et al. 2021).”
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- Authors
- Doolittle, W. F.
- Affiliations
- Dalhousie University (CA)
- Corresponding author
- Doolittle, W. Ford
- ORCID
- Doolittle, W. Ford
- Venue
- Current Biology
- Publisher
- Elsevier BV
- Published
- 2020-02
- Volume
- 30
- Issue
- 4
- Pages
- R177-R179
- ISSN
- 0960-9822 (ISSN-L)
- OpenAlex
- W3007830711
- Semantic Scholar
- c8ebd5ed55fe8cfa8eec63948a9b288e9c9c4921
- Cited by
- 75
- References
- 19
- Citations by year
- 2025: 5 · 2024: 4 · 2023: 36 · 2022: 22 · 2021: 5 · 2020: 3
- FWCI
- 4.56
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Current Biology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Natural Sciences and Engineering Research Council of Canada
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protist diversity and phylogeny
- Physical Sciences › Physics and Astronomy › Astronomy and Astrophysics › Origins and Evolution of Life
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
- Keywords
- eukaryotic origin, archaea, bacteria, eukaryotes
- MeSH terms
- Archaea, Bacteria, Biological Evolution, Phylogeny, Eukaryota
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