GABAA receptor subunit mRNA expression in cultured embryonic and adult human dorsal root ganglion neurons
Abstract
Previous studies have demonstrated significant pharmacological differences between the GABA(A) receptors expressed by neurons cultured from embryonic and adult human dorsal root ganglia (DRG). GABA(A) receptors of both embryonic and adult neurons are potentiated by diazepam and low concentrations of pentobarbital, and are activated by high concentrations of pentobarbital. However, in contrast to the GABA responses of embryonic neurons, the GABA responses of adult neurons are insensitive to both bicuculline and picrotoxin. We performed RT-PCR using subunit specific primer pairs, followed by Southern blot analysis with a third specific primer, to determine the pattern of subunit mRNA expression in cultures of embryonic and adult human DRG neurons. alpha2 and beta3 mRNA were expressed in all embryonic and adult cultures, while beta2 mRNA was present in all adult cultures but none of the embryonic cultures. Transcripts expressed by at least half of both embryonic and adult cultures were alpha3, alpha5, gamma2S, gamma3, theta, and rho1. Transcripts for gamma1 and delta were expressed in most adult cultures, but only a single embryonic culture. alpha4 mRNA was expressed by a single embryonic culture and pi mRNA was expressed by a single adult culture. We found no evidence for expression of alpha1, alpha6, beta1, gamma2L or rho2 transcripts. Changes in receptor subunit composition may underlie the novel pharmacological properties of GABA(A) receptor responses in adult cells. However…
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DRG neurons lack α1 and γ2L subunits.
“Most notably, DRG neurons lack the a1and g2L-subunits (Maddox et al., 2004)”
From The insecticide fipronil and its metabolite fipronil sulphone inhibit the rat α1β2γ2L GABA<sub>A</sub>receptor (2008) · cites this paper as Maddox et al., 2004
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Li, P., & Akk, G. (2008) · British Journal of Pharmacology · doi:10.1038/bjp.2008.309 · Cited by 33 · see citing works
- Authors
- Maddox, F. N., Valeyev, A. Y., Poth, K., Holohean, A. M., Wood, P. M., Davidoff, R. A., Hackman, J. C., & Luetje, C. W.
- Affiliations
- University of Miami (US)
- Corresponding author
- Luetje, Charles W
- Venue
- Developmental Brain Research
- Publisher
- Elsevier BV
- Published
- 2004-04
- Volume
- 149
- Issue
- 2
- Pages
- 143-151
- ISSN
- 0165-3806 (ISSN-L)
- OpenAlex
- W1964235903
- Cited by
- 43
- References
- 0
- Citations by year
- 2026: 4 · 2024: 1 · 2022: 1 · 2021: 3 · 2020: 2 · 2019: 3 · 2018: 1 · 2017: 1 · 2016: 2 · 2015: 2 · 2014: 6 · 2013: 3 · 2012: 2
- FWCI
- 0.47
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Developmental Brain Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01ns037946-04)
- National Institute on Drug Abuse (DA08102, R01DA008102)
- National Institute of Neurological Disorders and Stroke (NS 37946)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nicotinic Acetylcholine Receptors Study
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
- Keywords
- GABAA receptor, receptor subunit mRNA expression, dorsal root ganglion neurons, embryonic neurons, adult neurons, human neurons, diazepam potentiation, pentobarbital, subunit composition, gamma subunit, alpha subunit, post-translational modification
- MeSH terms
- Aging, Central Nervous System Stimulants, Bicuculline, Cells, Cultured, Drug Interactions, Embryo, Mammalian, gamma-Aminobutyric Acid, Ganglia, Spinal, Humans, Membrane Potentials, Neurons, Picrotoxin, Receptors, GABA-A, RNA, Messenger, Blotting, Southern, Gene Expression, Patch-Clamp Techniques, GABA Antagonists, Reverse Transcriptase Polymerase Chain Reaction, Protein Subunits
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