SMAUG Is a Major Regulator of Maternal mRNA Destabilization in Drosophila and Its Translation Is Activated by the PAN GU Kinase
Open accessAbstract
In animals, egg activation triggers a cascade of posttranscriptional events that act on maternally synthesized RNAs. We show that, in Drosophila, the PAN GU (PNG) kinase sits near the top of this cascade, triggering translation of SMAUG (SMG), a multifunctional posttranscriptional regulator conserved from yeast to humans. Although PNG is required for cytoplasmic polyadenylation of smg mRNA, it regulates translation via mechanisms that are independent of its effects on the poly(A) tail. Analyses of mutants suggest that PNG relieves translational repression by PUMILIO (PUM) and one or more additional factors, which act in parallel through the smg mRNA's 3' untranslated region (UTR). Microarray-based gene expression profiling shows that SMG is a major regulator of maternal transcript destabilization. SMG-dependent mRNAs are enriched for gene ontology annotations for function in the cell cycle, suggesting a possible causal relationship between failure to eliminate these transcripts and the cell cycle defects in smg mutants.
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In early Drosophila embryo data sets measuring translation, stability, and localization, the authors obtained dozens of significant associations between the presence of motif matches and specific regulatory outcomes.
“we have examined data sets measuring translation40, stability41 and localization42 of transcripts in the early Drosophila embryo, obtaining dozens of significant associations between the presence of motif matches and specific regulatory outcomes”
From A compendium of RNA-binding motifs for decoding gene regulation (2013) · cites this paper as [41]
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References 63 · Cited by 330
- Authors
- Tadros, W., Goldman, A. L., Babak, T., Menzies, F., Vardy, L., Orr-Weaver, T., Hughes, T. R., Westwood, J. T., Smibert, C. A., & Lipshitz, H. D.
- Affiliations
- University of Toronto (CA)
- Massachusetts Institute of Technology (US)
- Corresponding author
- Lipshitz, Howard D.
- Venue
- Developmental Cell
- Publisher
- Elsevier BV
- Published
- 2007-01
- Volume
- 12
- Issue
- 1
- Pages
- 143-155
- ISSN
- 1534-5807 (ISSN-L)
- OpenAlex
- W2013782508
- Cited by
- 330
- References
- 63
- Citations by year
- 2026: 7 · 2025: 9 · 2024: 19 · 2023: 15 · 2022: 17 · 2021: 12 · 2020: 15 · 2019: 15 · 2018: 24 · 2017: 20 · 2016: 15 · 2015: 18 · 2014: 21 · 2013: 17 · 2012: 17
- FWCI
- 9.41
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Developmental Cell (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Uniwersytet Medyczny im. Karola Marcinkowskiego w Poznaniu
- National Institute of General Medical Sciences (GM 39341, R01GM039341)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Keywords
- Smaug, oocyte activation, post-transcriptional regulation, translational regulation, Pumilio, 3' untranslated region, cytoplasmic polyadenylation, poly(A) tail, Drosophila, cell cycle defects
- MeSH terms
- Animals, Cytoplasm, Drosophila melanogaster, Embryo, Nonmammalian, Female, Heat-Shock Proteins, Models, Genetic, Mutation, Ovum, Repressor Proteins, Protein Biosynthesis, RNA-Binding Proteins, Protein Serine-Threonine Kinases, Gene Expression Regulation, Developmental, Computational Biology, 3' Untranslated Regions, Gene Expression Profiling, RNA Stability, RNA, Messenger, Stored, Polyadenylation, Drosophila Proteins, Microarray Analysis
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