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Cited by 71Nuclear Structure and FunctionMicrotubule and mitosis dynamicsMuscle Physiology and Disorders

Amphiphysin 2 Orchestrates Nucleus Positioning and Shape by Linking the Nuclear Envelope to the Actin and Microtubule Cytoskeleton

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D’Alessandro, Manuela, Hnia, Karim, Gache, Vincent, Koch, Catherine, Gavriilidis, Christos, Rodriguez, David, Nicot, Anne-Sophie, Romero, Norma B. +4 more·2015·Developmental Cell
DOI 10.1016/j.devcel.2015.09.018
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Abstract

Nucleus positioning is key for intracellular organization, cell differentiation, and organ development and is affected in many diseases, including myopathies due to alteration in amphiphysin-2 (BIN1). The actin and microtubule cytoskeletons are essential for nucleus positioning, but their crosstalk in this process is sparsely characterized. Here, we report that impairment of amphiphysin/BIN1 in Caenorhabditis elegans, mammalian cells, or muscles from patients with centronuclear myopathy alters nuclear position and shape. We show that AMPH-1/BIN1 binds to nesprin and actin, as well as to the microtubule-binding protein CLIP170 in both species. Expression of the microtubule-anchoring CAP-GLY domain of CLIP170 fused to the nuclear-envelope-anchoring KASH domain of nesprin rescues nuclear positioning defects of amph-1 mutants. Amphiphysins thus play a central role in linking the nuclear envelope with the actin and microtubule cytoskeletons. We propose that BIN1 has a direct and evolutionarily conserved role in nuclear positioning, altered in myopathies.

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Authors
D’Alessandro, M., Hnia, K., Gache, V., Koch, C., Gavriilidis, C., Rodriguez, D., Nicot, A.-S., Romero, N. B., Schwab, Y., Gomes, E., Labouesse, M., & Laporte, J.
Affiliations
  • Lyon 1 Université (FR)
  • Centre National de la Recherche Scientifique (FR)
  • École Normale Supérieure de Lyon (FR)
  • Institut de Myologie (FR)
  • University of Lisbon (PT)
Corresponding author
  • D’Alessandro, Manuela
  • Laporte, Jocelyn
ORCID
Venue
Developmental Cell
Publisher
Elsevier BV
Published
2015-10
Volume
35
Issue
2
Pages
186-198
ISSN
1534-5807 (ISSN-L)
DOI
10.1016/j.devcel.2015.09.018
OpenAlex
W1870115160
Cited by
71
References
2
Citations by year
2026: 2 · 2025: 9 · 2024: 3 · 2023: 3 · 2022: 5 · 2021: 10 · 2020: 6 · 2019: 8 · 2018: 10 · 2017: 10 · 2016: 5
FWCI
3.29
Type
journal article
Language
en
Open access
Licence
Locations
  • Developmental Cell (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
Funders
Agence Nationale de la Recherche (ANR-10-LABX-0030)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
Keywords
BIN1, nuclear positioning, nuclear shape, nuclear envelope, actin cytoskeleton, microtubule cytoskeleton, nesprins, centronuclear myopathy, myopathy, KASH domain, CAP-Gly domain, Caenorhabditis elegans
MeSH terms
Actins, Animals, Cell Nucleus, Chlorocebus aethiops, Cytoplasm, Cytoskeleton, Humans, Microfilament Proteins, Microtubule-Associated Proteins, Microtubules, Neoplasm Proteins, Nerve Tissue Proteins, Nuclear Envelope, Nuclear Proteins, Caenorhabditis elegans, Muscle, Skeletal, COS Cells, Myopathies, Structural, Congenital, Multiprotein Complexes, Cell Shape, HEK293 Cells

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