The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
A Rapid Method for Directed Gene Knockout for Screening in G0 Zebrafish
Open accessAbstract
Zebrafish is a powerful model for forward genetics. Reverse genetic approaches are limited by the time required to generate stable mutant lines. We describe a system for gene knockout that consistently produces null phenotypes in G0 zebrafish. Yolk injection of sets of four CRISPR/Cas9 ribonucleoprotein complexes redundantly targeting a single gene recapitulated germline-transmitted knockout phenotypes in >90% of G0 embryos for each of 8 test genes. Early embryonic (6 hpf) and stable adult phenotypes were produced. Simultaneous multi-gene knockout was feasible but associated with toxicity in some cases. To facilitate use, we generated a lookup table of four-guide sets for 21,386 zebrafish genes and validated several. Using this resource, we targeted 50 cardiomyocyte transcriptional regulators and uncovered a role of zbtb16a in cardiac development. This system provides a platform for rapid screening of genes of interest in development, physiology, and disease models in zebrafish.
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Cites
The zebrafish reference genome sequence and its relationship to the human genome.
Howe, K., Clark, M. D., Torroja, C. F., Torrance, J., Berthelot, C., Muffato, M., Collins, J. E., Humphray, S., McLaren, K., Matthews, L., et al. (2013) · Nature · doi:10.1038/nature12111 · Cited by 5,100
Efficient genome editing in zebrafish using a CRISPR-Cas system
Hwang, W. Y., Fu, Y., Reyon, D., Maeder, M. L., Tsai, S. Q., Sander, J. D., Peterson, R. T., Yeh, J.-R. J., & Joung, J. K. (2013) · Nature Biotechnology · doi:10.1038/nbt.2501 · Cited by 2,937
Crystal structure of Cas9 in complex with guide RNA and target DNA
Nishimasu, H., Ran, F. A., Hsu, P. D., Konermann, S., Shehata, S. I., Dohmae, N., Ishitani, R., Zhang, F., & Nureki, O. (2014) · Cell · doi:10.1016/j.cell.2014.02.001 · Cited by 2,306
CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting in vivo
Moreno-Mateos, M. A., Vejnar, C. E., Beaudoin, J.-D., Fernandez, J. P., Mis, E. K., Khokha, M. K., & Giraldez, A. J. (2015) · Nature Methods · doi:10.1038/nmeth.3543 · Cited by 1,290
SLC24A5, a putative cation exchanger, affects pigmentation in zebrafish and humans
Lamason, R. L., Mohideen, M.-A. P. K., Mest, J. R., Wong, A. C., Norton, H. L., Aros, M. C., Jurynec, M. J., Mao, X., Humphreville, V. R., Humbert, J. E., et al. (2005) · Science · doi:10.1126/science.1116238 · Cited by 1,043
Cited by
Penalva-Tena, A., Bedke, J., Gaudin, A., Barrios, J. P., Bertram, E. P. L., & Douglass, A. D. (2018) · doi:10.1101/444026 · Cited by 41
Quick, R. E., Buck, L. D., Parab, S., Tolbert, Z. R., & Matsuoka, R. L. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.735598 · Cited by 32
Cold shock domain-containing protein E1 is a posttranscriptional regulator of the LDL receptor
Smith, G. A., Padmanabhan, A., Lau, B. H., Pampana, A., Li, L., Lee, C. Y., Pelonero, A., Nishino, T., Sadagopan, N., Xia, V. Q., et al. (2022) · Science Translational Medicine · doi:10.1126/scitranslmed.abj8670 · Cited by 23
Self-citationWang, Y., Jiang, Z., Deng, L., Zhang, G., Xu, X., Alonge, E., Zhang, H., & Guo, C. (2023) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2023.126600 · Cited by 22
Chau, T. C. Y., Keyser, M. S., Da Silva, J. A., Morris, E. K., Yordanov, T. E., Duscyz, K. P., Paterson, S., Yap, A. S., Hogan, B. M., & Lagendijk, A. K. (2022) · Development · doi:10.1242/dev.200454 · Cited by 18
Metadata
- Authors
- Wu, R. S., Lam, I. I., Clay, H., Duong, D. N., Deo, R. C., & Coughlin, S. R.
- Venue
- Developmental Cell
- Publisher
- Elsevier BV
- Published
- 2018-07
- Volume
- 46
- Issue
- 1
- Pages
- 112-125.e4
- ISSN
- 1534-5807 (ISSN-L)
- OpenAlex
- W2810058136
- Cited by
- 398
- References
- 5
- FWCI
- 16.85
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Wu, R. S., Lam, I. I., Clay, H., Duong, D. N., Deo, R. C., & Coughlin, S. R. (2018). A Rapid Method for Directed Gene Knockout for Screening in G0 Zebrafish. Developmental Cell. https://doi.org/10.1016/j.devcel.2018.06.003
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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