The DOI matches its Crossref record; 17 of the 17 references it cites were found in a registry or an index.
Real-time PCR for detection of Streptococcus suis serotype 2 in cerebrospinal fluid of human patients with meningitis
Open accessAbstract
Streptococcus suis serotype 2 is an emerging zoonotic pathogen and is the main cause of acute bacterial meningitis in adult patients in Vietnam. We developed an internally controlled real-time PCR for detection of S. suis serotype 2 in cerebrospinal fluid (CSF) samples targeted at the cps2J gene. Sensitivity and specificity in culture-confirmed clinical samples were 100%. The PCR detected S. suis serotype 2 infection in 101 of 238 (42.4%) prospectively collected CSF samples, of which 55 (23%) were culture positive. Culture-negative but PCR-positive CSF samples were significantly associated with the use of antimicrobial agents before admission. S. suis serotype 2 infection was more common than infections with Streptococcus pneumoniae and Neisseria meningitidis combined. Our results strikingly illustrate the additional diagnostic value of PCR in patients who are pretreated with antimicrobial agents and demonstrate the extremely high prevalence of S. suis infections among Vietnamese adult patients with bacterial meningitis.
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The addition of PCR increased the diagnostic yield for S. suis meningitis by 84%.
“The addition of PCR in this population increased the diagnostic yield for S. suis meningitis by 84%.”
From Molecular diagnostics in cerebrospinal fluid for the diagnosis of central nervous system infections (2024) · cites this paper as [80]
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Self-citationStreptococcal Toxic Shock Syndrome Caused by Streptococcus suis Serotype 2
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Molecular diagnostics in cerebrospinal fluid for the diagnosis of central nervous system infections
Olie, S. E., Andersen, C. Ø., van de Beek, D., & Brouwer, M. C. (2024) · Clinical Microbiology Reviews · doi:10.1128/cmr.00021-24 · Cited by 38
“One study analyzing the performance of this PCR target included 114 culture-confirmed S. suis serotype 2 cases and 99 controls consisting of patients with bacterial meningitis due to other pathogens (80).”
Metadata
- Authors
- Nga, T. V. T., Nghia, H. D. T., Tu, L. T. P., Diep, T. S., Mai, N. T. H., Chau, T. T. H., Sinh, D. X., Phu, N. H., Nga, T. T. T., Chau, N. V. V., Campbell, J., Hoa, N. T., Chinh, N. T., Hien, T. T., Farrar, J., & Schultsz, C.
- Affiliations
- Oxford University Clinical Research Unit (VN)
- Pham Ngoc Thach University of Medicine (VN)
- Amsterdam UMC Location University of Amsterdam (NL)
- Corresponding author
- Schultsz, Constance
- ORCID
- Venue
- Diagnostic Microbiology and Infectious Disease
- Publisher
- Elsevier BV
- Published
- 2011-08
- Volume
- 70
- Issue
- 4
- Pages
- 461-467
- ISSN
- 0732-8893 (ISSN-L)
- PMC
- PMC3146703
- OpenAlex
- W2006391185
- Cited by
- 50
- References
- 17
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 2 · 2023: 1 · 2022: 4 · 2021: 2 · 2020: 1 · 2019: 1 · 2018: 3 · 2017: 2 · 2016: 5 · 2015: 6 · 2014: 6 · 2013: 5 · 2012: 5
- FWCI
- 5.34
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Diagnostic Microbiology and Infectious Disease (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Pure Amsterdam UMC (publishedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Greater South Information System
- Funders
- Wellcome Trust
- Topics
- Life Sciences › Immunology and Microbiology › Microbiology › Bacterial Infections and Vaccines
- Health Sciences › Medicine › Microbiology (medical) › Bacterial Identification and Susceptibility Testing
- Health Sciences › Medicine › Infectious Diseases › Streptococcal Infections and Treatments
- Keywords
- Streptococcus suis serotype 2, bacterial meningitis, cerebrospinal fluid, real-time PCR, zoonotic pathogens, culture-negative meningitis, Streptococcus pneumoniae, Neisseria meningitidis, sensitivity and specificity, Vietnam
- MeSH terms
- Adult, Bacteriological Techniques, Cerebrospinal Fluid, Female, Humans, Male, Middle Aged, Sensitivity and Specificity, Streptococcal Infections, Vietnam, Prevalence, Polymerase Chain Reaction, Meningitis, Bacterial, Streptococcus suis
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