Comparison of Cobas® HPV and Anyplex™ II HPV28 assays for detecting and genotyping human papillomavirus
Abstract
Persistent infection with high-risk human papillomavirus (HPV-HR) is a recognized cause of cervical cancer. The aim of this study was to compare analytical and clinical performances of the Cobas® HPV and Anyplex™ II HPV28 assays for HPV detection and genotyping. A total of 94 cervical samples were tested. For HPV-HR, the results agreed very well (94.68%), with 100% agreement when detecting CIN2+. The Anyplex™ II HPV28 assay detected more genotypes than the Cobas® HPV Test, but their clinical performances were similar.
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Clinical validation of Onclarity and Anyplex has been performed in previous studies according to international guidelines.
“Although clinical validation of Onclarity and Anyplex has been performed in previous studies according to the international guidelines for human papillomavirus DNA testing [13–17]”
From Comparison of Seegene Anyplex II HPV28 assay with BD Onclarity HPV assay for human papillomavirus genotyping (2022) · cites this paper as [17]
Cites
Hesselink, A. T., Sahli, R., Berkhof, J., Snijders, P. J. F., van der Salm, M. L., Agard, D., Bleeker, M. C. G., & Heideman, D. A. M. (2016) · Journal of Clinical Virology · doi:10.1016/j.jcv.2016.01.009 · Cited by 61 · see citing works
Comparison of Seegene Anyplex II HPV28 with the PGMY-CHUV Assay for Human Papillomavirus Genotyping
Estrade, C., & Sahli, R. (2014) · Journal of Clinical Microbiology · doi:10.1128/jcm.02749-13 · Cited by 57 · see citing works
Evaluation of HPV Genotyping Assays for Archival Clinical Samples
Lillsunde Larsson, G., Carlsson, J., Karlsson, M. G., & Helenius, G. (2015) · The Journal of Molecular Diagnostics · doi:10.1016/j.jmoldx.2014.12.004 · Cited by 49 · see citing works
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Bell, M., Verberckmoes, B., Devolder, J., Vermandere, H., Degomme, O., Guimarães, Y. M., Godoy, L. R., Ambrosino, E., Cools, P., & Padalko, E. (2023) · Microbiology Spectrum · doi:10.1128/spectrum.00081-23 · Cited by 22 · see citing works
“In the Anyplex II HPV28 detection assay, the HPV load was categorized as either high (111), medium (11), or low (1), if the Cq value was #31.”
BackgroundMethodKim, M., Kim, J., Park, N. J.-Y., & Park, J. Y. (2022) · PLOS ONE · doi:10.1371/journal.pone.0267836 · Cited by 5 · see citing works
- Authors
- Pasquier, C., Sauné, K., Raymond, S., Boisneau, J., Courtade, M., & Izopet, J.
- Affiliations
- Université Toulouse III - Paul Sabatier (FR)
- Centre Hospitalier Universitaire de Toulouse (FR)
- Corresponding author
- Pasquier, Christophe
- Venue
- Diagnostic Microbiology and Infectious Disease
- Publisher
- Elsevier BV
- Published
- 2017-01
- Volume
- 87
- Issue
- 1
- Pages
- 25-27
- ISSN
- 0732-8893 (ISSN-L)
- OpenAlex
- W2514792000
- Cited by
- 9
- References
- 3
- Citations by year
- 2025: 2 · 2024: 1 · 2023: 1 · 2022: 1 · 2019: 3 · 2018: 1
- FWCI
- 1.25
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Diagnostic Microbiology and Infectious Disease (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Epidemiology › Cervical Cancer and HPV Research
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Women's cancer prevention and management
- Keywords
- human papillomavirus, HPV genotyping, HPV testing, cobas HPV test, assay comparison, cervical intraepithelial neoplasia, cervical cancer, high-risk HPV, cervical samples
- MeSH terms
- Adult, Aged, Female, Humans, Middle Aged, Molecular Diagnostic Techniques, Papillomaviridae, Papillomavirus Infections, Young Adult, Genotyping Techniques
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