Antidiabetic activity of γ-sitosterol isolated from Lippia nodiflora L. in streptozotocin induced diabetic rats
Abstract
Lippia nodiflora L. (Verbenaceae) is a creeping perennial herb widely used in traditional system of medicine to treat ulcers, bronchitis and heart diseases; it also possesses antidiabetic property. In the present study, γ-sitosterol isolated from Lippia nodiflora was screened for its antidiabetic property in streptozotocin (STZ) induced diabetic rats. Insulin secretion in response to glucose was evaluated in isolated rat islets. Oral administration of γ-sitosterol (20 mg/kg body weight) once daily for 21 days in STZ-induced diabetic rats resulted in a significant decrease in blood glucose and glycosylated hemoglobin with a significant increase in plasma insulin level, body weight and food intake. Furthermore γ-sitosterol showed antihyperlipidemic activity as evidenced by significant decrease in serum total cholesterol, triglycerides and very low density lipoprotein-cholesterol levels coupled with elevation of high density lipoprotein-cholesterol levels in treated rats. A significant decrease in the activities of alanine aminotransaminase, aspartate aminotransaminase, alkaline phosphatase and acid phosphatase in γ-sitosterol treated rats when compared to diabetic control rats indicated its protective role against liver damage. γ-Sitosterol increased insulin secretion in response to glucose. Immunohistochemical study of pancreas also confirmed the biochemical findings. These results indicated that γ-sitosterol, the compound isolated from L. nodiflora, possessed antihyperglycemic activity.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Gamma-sitosterol is known for anti-diabetic activities and is a potent inhibitor of complement C1 component.
“First, gamma sitosterol (steroid) was known for its anti diabetic activities32 and known potent inhibitor of complement C1 component33.”
From Bioactivities of Allium longicuspis Regel against anthracnose of mango caused by Colletotrichum gloeosporioides (Penz.) (2020) · cites this paper as [32]
Cites
Friedewald, W. T., Levy, R. I., & Fredrickson, D. S. (1972) · Clinical Chemistry · doi:10.1093/clinchem/18.6.499 · Cited by 32,423 · see citing works
(1975) · Journal of AOAC INTERNATIONAL · doi:10.1093/jaoac/58.5.1085 · Cited by 20,078 · see citing works
Hsu, S. M., Raine, L., & Fanger, H. (1981) · Journal of Histochemistry & Cytochemistry · doi:10.1177/29.4.6166661 · Cited by 14,248 · see citing works
Reitman, S., & Frankel, S. (1957) · American Journal of Clinical Pathology · doi:10.1093/ajcp/28.1.56 · Cited by 10,015 · see citing works
Determination of Glucose in Blood Using Glucose Oxidase with an Alternative Oxygen Acceptor
Trinder, P. (1969) · Annals of Clinical Biochemistry International Journal of Laboratory Medicine · doi:10.1177/000456326900600108 · Cited by 4,629 · see citing works
THE DETERMINATION OF GLYCOGEN IN LIVER AND MUSCLE BY USE OF ANTHRONE REAGENT
Carroll, N. V., Longley, R. W., & Roe, J. H. (1956) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)65284-6 · Cited by 1,318 · see citing works
Correlation of Glucose Regulation and Hemoglobin A Ic in Diabetes Mellitus
Koenig, R. J., Peterson, C. M., Jones, R. L., Saudek, C. D., Lehrman, M. A., & Cerami, A. (1976) · New England Journal of Medicine · doi:10.1056/nejm197608192950804 · Cited by 1,173 · see citing works
Antidiabetic plants and their active constituents
Marles, R. J., & Farnsworth, N. R. (1995) · Phytomedicine · doi:10.1016/s0944-7113(11)80059-0 · Cited by 1,105 · see citing works
Natural medicines used in the traditional Chinese medical system for therapy of diabetes mellitus
Li, W., Zheng, H. C., Bukuru, J., & KIMPE, N. D. (2004) · Journal of Ethnopharmacology · doi:10.1016/j.jep.2003.12.031 · Cited by 970 · see citing works
Diabetogenic action of streptozotocin: relationship of dose to metabolic response
Junod, A. F., Lambert, A. E., Stauffacher, W., & Renold, A. E. (1969) · Journal of Clinical Investigation · doi:10.1172/jci106180 · Cited by 931 · see citing works
Practical Clinical Enzymology.
(1966) · Annals of Internal Medicine · doi:10.7326/0003-4819-64-2-485_2 · Cited by 869 · see citing works
Lippia: traditional uses, chemistry and pharmacology: a review
Pascual, M. E., Slowing, K., Carretero, E., Mata, D. S., & Villar, A. (2001) · Journal of Ethnopharmacology · doi:10.1016/s0378-8741(01)00234-3 · Cited by 658 · see citing works
The Biochemistry of the Complications of Diabetes Mellitus
Brownlee, M., & Cerami, A. (1981) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.50.070181.002125 · Cited by 419 · see citing works
Grover, J. K., Vats, V., & Rathi, S. S. (2000) · Journal of Ethnopharmacology · doi:10.1016/s0378-8741(00)00319-6 · Cited by 415 · see citing works
Al-Shamaony, L. A., Al-Khazraji, S. M., & Twaij, H. A. A. (1994) · Journal of Ethnopharmacology · doi:10.1016/0378-8741(94)90038-8 · Cited by 391 · see citing works
Isolation and culture of the islets of Langerhans of the guinea pig
Moskalewski, S. (1965) · General and Comparative Endocrinology · doi:10.1016/0016-6480(65)90059-6 · Cited by 347 · see citing works
A new colorimetric method for the estimation of glycosylated hemoglobin
Nayak, S. S., & Pattabiraman, T. N. (1981) · Clinica Chimica Acta · doi:10.1016/0009-8981(81)90312-0 · Cited by 333 · see citing works
Gupta, R., Sharma, A. K., DOBHAL, M. P., SHARMA, M. C., & GUPTA, R. S. (2010) · Journal of Diabetes · doi:10.1111/j.1753-0407.2010.00107.x · Cited by 329 · see citing works
The Antihyperglycaemic Effect of Metformin
Wiernsperger, N. F., & Bailey, C. J. (1999) · Drugs · doi:10.2165/00003495-199958001-00009 · Cited by 313 · see citing works
Pathological occurrence of glucose-6-phosphatase in serum in liver diseases
Koide, H., & Oda, T. (1959) · Clinica Chimica Acta · doi:10.1016/0009-8981(59)90165-2 · Cited by 276 · see citing works
Aybar, M. J., Riera, A. N. S., Grau, A., & Sánchez, S. S. (2001) · Journal of Ethnopharmacology · doi:10.1016/s0378-8741(00)00351-2 · Cited by 261 · see citing works
Free Radical Scavenger and Antihepatotoxic Activity ofRosmarinus tomentosus
Navarro, M., Montilla, M., Martín, A., Jiménez, J. L. M., & Utrilla, M. P. (1993) · Planta Medica · doi:10.1055/s-2006-959688 · Cited by 250 · see citing works
Plasma triglyceride transport kinetics in diabetes mellitus
Nikkilä, E. A., & KEKKI, M. (1973) · Metabolism · doi:10.1016/0026-0495(73)90024-3 · Cited by 234 · see citing works
Anti-hyperglycemic effect of black tea (Camellia sinensis) in rat
Gomes, A., Vedasiromoni, J. R., Das, M., Sharma, R., & Ganguly, D. K. (1995) · Journal of Ethnopharmacology · doi:10.1016/0378-8741(95)01223-z · Cited by 188 · see citing works
Ohaeri, O. (2001) · Bioscience Reports · doi:10.1023/a:1010425932561 · Cited by 183 · see citing works
Glucose-6-Phosphate Dehydrogenase
Luzzatto, L., & Battistuzzi, G. (1985) · Springer eBooks · doi:10.1007/978-1-4615-9400-0_4 · Cited by 173 · see citing works
Essential Oil Constituents of the GenusLippia(Verbenaceae)—A Literature Review
Terblanche, F. C., & Kornelius, G. (1996) · Journal of Essential Oil Research · doi:10.1080/10412905.1996.9700673 · Cited by 159 · see citing works
Dosage effect of streptozotocin on rat tissue enzyme activities and glycogen concentration
Chen, V., & Ianuzzo, C. D. (1982) · Canadian Journal of Physiology and Pharmacology · doi:10.1139/y82-183 · Cited by 143 · see citing works
Hassan, Z., Yam, M. F., Ahmad, M., et al. (2010) · Molecules · doi:10.3390/molecules15129008 · Cited by 140 · see citing works
Ugochukwu, N. H., & Babady, N. E. (2002) · Fitoterapia · doi:10.1016/s0367-326x(02)00218-6 · Cited by 119 · see citing works
Lowering of Serum Cholesterol by the Administration of a Plant Sterol
Best, M. M., Duncan, C. H., Loon, E. J. V., & Wathen, J. D. (1954) · Circulation · doi:10.1161/01.cir.10.2.201 · Cited by 116 · see citing works
Mitra, S. K., Gopumadhavan, S., Muralidhar, T. S., et al. (1995) · PubMed · Cited by 104 · see citing works
Potential new treatments for type 2 diabetes
Bailey, C. J. (2000) · Trends in Pharmacological Sciences · doi:10.1016/s0165-6147(00)01506-6 · Cited by 102 · see citing works
Glycogen synthesis by hepatocytes from diabetic rats
Golden, S., Wals, P. A., Okajima, F., & Katz, J. J. (1979) · Biochemical Journal · doi:10.1042/bj1820727 · Cited by 102 · see citing works
Hypoglycemic effects of Cecropia pachystachya in normal and alloxan-induced diabetic rats
Aragão, D. M. D. O., Guarize, L., Lanini, J., et al. (2010) · Journal of Ethnopharmacology · doi:10.1016/j.jep.2010.01.008 · Cited by 97 · see citing works
Extra-pancreatic effects of Momordica charantia in rats.
Welihinda, J., & Karunanayake, E. (1986) · Journal of Ethnopharmacology · doi:10.1016/0378-8741(86)90112-1 · Cited by 96 · see citing works
Glycosylated Hemoglobin and Diabetic Control
Gabbay, K. H. (1976) · New England Journal of Medicine · doi:10.1056/nejm197608192950810 · Cited by 89 · see citing works
Hypoglycemic effect and chlorogenic acid content in two Cecropia species
Nicasio-Torres, P., Aguilar‐Santamaría, L., Aranda, E., Ortiz, S., & González‐Cortazar, M. (2005) · Phytotherapy Research · doi:10.1002/ptr.1722 · Cited by 89 · see citing works
Hwang, S., Kim, H.-N., Jung, H.-H., Kim, J.-E., Choi, D.-K., Hur, J.-M., Lee, J.-Y., Song, H., Song, K., & Huh, T. (2008) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2008.10.136 · Cited by 77 · see citing works
Hisatomi, M., Hidaka, H., & Niki, I. (1996) · Endocrinology · doi:10.1210/endo.137.11.8895328 · Cited by 74 · see citing works
Cycloartane type triterpenoids from the rhizomes of Polygonum bistorta
Manoharan, K. P., Benny, T. K. H., & Yang, D. (2005) · Phytochemistry · doi:10.1016/j.phytochem.2005.07.008 · Cited by 70 · see citing works
Nagy, K. A., Kis, B., Rajapakse, N. C., Bari, F., & Busija, D. W. (2004) · Journal of Neuroscience Research · doi:10.1002/jnr.20120 · Cited by 67 · see citing works
Chen, R., Meseck, M. L., McEvoy, R. C., et al. (2000) · Gene Therapy · doi:10.1038/sj.gt.3301306 · Cited by 64 · see citing works
Twelve 6-oxygenated flavone sulphates from Lippia nodiflora andL. canescens
Tomás-Barberán, F. A., Harborne, J. B., & Self, R. (1987) · Phytochemistry · doi:10.1016/s0031-9422(00)84701-9 · Cited by 64 · see citing works
Antistress Activity ofTinospora cordifoliaandCentella asiaticaExtracts
Sarma, D. N. K., Khosa, R. L., Chansauria, J. P. N., & Sahai, M. (1996) · Phytotherapy Research · doi:10.1002/(sici)1099-1573(199603)10:2 · Cited by 60 · see citing works
New insight into the regulation of liver glycogen metabolism by glucose
Stalmans, W., Cadefau, J. A., Wera, S., et al. (1997) · Biochemical Society Transactions · doi:10.1042/bst0250019 · Cited by 48 · see citing works
Komatsu, M., Noda, M., & Sharp, G. W. G. (1998) · Endocrinology · doi:10.1210/endo.139.3.5859 · Cited by 42 · see citing works
Jain, P., Bari, S. B., & Surana, S. J. (2009) · Asian Journal of Biological Sciences · doi:10.3923/ajbs.2009.112.117 · Cited by 34 · see citing works
Streptozotocin Induces Lipolysis in Rat Adipocytes in Vitro.
Szkudelski, T., & Szkudelska, K. (2002) · Physiological Research · doi:10.33549/physiolres.930183 · Cited by 34 · see citing works
Abdel‐Rahim, E. A., El-Saadany, S. S., Abo-Aytta, A. M., & Wasi, M. (1992) · Food / Nahrung · doi:10.1002/food.19920360103 · Cited by 33 · see citing works
The biochemistry of the complications of diabetes mellitus
Kg, A. (1982) · Medical Entomology and Zoology · Cited by 26 · see citing works
JTT‐608 restores impaired early insulin secretion in diabetic Goto‐Kakizaki rats
Ohta, T., Furukawa, N., Komuro, G., Yonemori, F., & Wakitani, K. (1999) · British Journal of Pharmacology · doi:10.1038/sj.bjp.0702481 · Cited by 20 · see citing works
Durairaj, A. K., Vaiyapuri, T. S., Kanti, M. U., et al. (2008) · Cited by 20 · see citing works
Hisatomi, M. (1996) · Endocrinology · doi:10.1210/en.137.11.4644 · Cited by 17 · see citing works
BTS 67 582 stimulates insulin secretion from perifused rat pancreatic islets
Dickinson, K., North, T. J., Sills, S., et al. (1997) · European Journal of Pharmacology · doi:10.1016/s0014-2999(97)01356-3 · Cited by 16 · see citing works
Gastroprotective effect of Lippia nodiflora L. extracts in ethanol- induced gastric lesions
Khalil, H. E., Ismail, H. T. H., Taye, A., et al. (2007) · Pharmacognosy Magazine · Cited by 13 · see citing works
Glycosylated haemoglobin and diabetic control.
Thai, A. C., Yeo, P. P., Chan, L., et al. (1983) · PubMed · Cited by 13 · see citing works
Komatsu, M. (1998) · Endocrinology · doi:10.1210/en.139.3.1172 · Cited by 7 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
de Guzman Alvindia, D., & Mangoba, M. A. A. (2020) · Scientific Reports · doi:10.1038/s41598-020-68399-z · Cited by 18 · see citing works
- Authors
- Balamurugan, R., Duraipandiyan, V., & Ignacimuthu, S.
- Affiliations
- Loyola College (IN)
- Venue
- European Journal of Pharmacology
- Publisher
- Elsevier BV
- Published
- 2011-09
- Volume
- 667
- Issue
- 1-3
- Pages
- 410-418
- ISSN
- 0014-2999 (ISSN-L)
- OpenAlex
- W2065105404
- Cited by
- 173
- References
- 58
- Citations by year
- 2026: 11 · 2025: 17 · 2024: 25 · 2023: 13 · 2022: 17 · 2021: 15 · 2020: 4 · 2019: 10 · 2018: 11 · 2017: 4 · 2016: 8 · 2015: 11 · 2014: 12 · 2013: 9 · 2012: 5
- FWCI
- 7.08
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- European Journal of Pharmacology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Indian Council of Medical Research
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Natural Antidiabetic Agents Studies
- Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Medicinal Plant Research
- Keywords
- γ-sitosterol, Lippia nodiflora, antidiabetic activity, streptozotocin-induced diabetes, insulin secretion, pancreatic islets, hypolipidemic activity, serum lipids, hepatoprotection, HbA1c, traditional medicine
- MeSH terms
- Insulin Secretion, Animals, Blood Glucose, Body Weight, Diabetes Mellitus, Experimental, Fasting, Glycogen, Glycated Hemoglobin, Hypoglycemic Agents, Insulin, Liver, Male, Muscles, Sitosterols, Biomarkers, Rats, Wistar, Lippia, Carbohydrate Metabolism, Lipid Metabolism, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review