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Cited by 39Effects and risks of endocrine disrupting chemicalsToxic Organic Pollutants ImpactEnvironmental Toxicology and Ecotoxicology

The DOI matches its Crossref record; 45 of the 45 references it cites were found in a registry or an index.

Alteration of steroidogenesis in H295R cells by organic sediment contaminants and relationships to other endocrine disrupting effects

Bláha, Luděk, Hilscherová, Klára, Mazurová, Edita, Hecker, Markus, Jones, Paul D., Newsted, John L., Bradley, Patrick W., Gracia, Tannia, Ďuriš, Zdenek, Horká, Ivona, Holoubek, Ivan, Giesy, John P.·2006·Environment International
DOI 10.1016/j.envint.2006.03.011
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Abstract

A novel bioassay with the human adrenocortical carcinoma cell line H295R can be used to screen for endocrine disrupting chemicals that affect the expression of genes important in steroidogenesis. This assay was employed to study the effects of organic contaminants associated with the freshwater pond sediments collected in the Ostrava-Karvina region, Czech Republic. The modulation of ten major genes involved in the synthesis of steroid hormones (CYP11A, CYP11B2, CYP17, CYP19, 17betaHSD1, 17betaHSD4, CYP21, 3betaHSD2, HMGR, StAR) after exposure of H295R cells to sediment extracts was investigated using quantitative real-time polymerase chain reaction (PCR). Crude sediment extracts, containing high concentrations of polycyclic aromatic hydrocarbons (PAHs) and moderate amounts of polychlorinated biphenyls (PCBs) and organochlorine pesticides (OCPs) significantly stimulated expression of the CYP11B2 gene (up to 10-fold induction), and suppressed expression of 3betaHSD2 and CYP21 genes. A similar pattern was observed with the extracts after treatment with concentrated sulfuric acid to remove labile chemicals (including PAHs) leaving only persistent PCBs, OCPs and potentially PCDD/Fs. Comparison of the results with other mechanistically based bioassays (arylhydrocarbon receptor, AhR, mediated responses in H4IIE-luc cells, and estrogen receptor mediated effects in MVLN cells) revealed significant endocrine disrupting potencies of organic contaminants present in the sediments (most likely antiestrogenicity). Pronounced effects were observed particularly in sediment extracts from the Pilnok Pond which harbors an unusual intersexual population of the narrow-cawed crayfish Pontastacus leptodactylus (Decapoda, Crustacea). This pilot study provided the first experimental evidence of the wider application of the H295R bioassay for screening complex environmental samples, and the results support the hypothesis of chemical-induced endocrine disruption in intersexual crayfish.

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Crossref · 10.1016/j.envint.2006.03.011

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Metadata

Authors
Bláha, L., Hilscherová, K., Mazurová, E., Hecker, M., Jones, P. D., Newsted, J. L., Bradley, P. W., Gracia, T., Ďuriš, Z., Horká, I., Holoubek, I., & Giesy, J. P.
Affiliations
  • Masaryk University (CZ)
  • Michigan State University (US)
  • University of Ostrava (CZ)
  • City University of Hong Kong (HK)
Corresponding author
Bláha, Luděk
ORCID
Venue
Environment International
Publisher
Elsevier BV
Published
2006-08
Volume
32
Issue
6
Pages
749-757
ISSN
0160-4120 (ISSN-L)
DOI
10.1016/j.envint.2006.03.011
OpenAlex
W2015734637
Cited by
39
References
45
Citations by year
2022: 1 · 2021: 3 · 2018: 2 · 2017: 3 · 2015: 3 · 2014: 2 · 2013: 2 · 2012: 2
FWCI
1.78
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Environment International (publishedVersion)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
Masarykova Univerzita
Topics
  • Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Effects and risks of endocrine disrupting chemicals
  • Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Toxic Organic Pollutants Impact
  • Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Environmental Toxicology and Ecotoxicology
Keywords
endocrine disruptors, steroidogenesis, H295R cells, polycyclic aromatic hydrocarbons, polychlorinated biphenyls, organochlorine pesticides, CYP11B2, CYP21, Pontastacus leptodactylus, crayfish, sediment extracts
MeSH terms
Steroidogenic Acute Regulatory Protein, Humans, Hydrocarbons, Chlorinated, Insecticides, Oxidoreductases, Phosphoproteins, Polycyclic Aromatic Hydrocarbons, Receptors, Estrogen, Water Pollutants, Chemical, Gene Expression, Receptors, Aryl Hydrocarbon, Geologic Sediments, Cell Line, Tumor, Endocrine Disruptors

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