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Cited by 41Microplastics and Plastic PollutionEnvironmental Toxicology and EcotoxicologyPharmaceutical and Antibiotic Environmental Impacts

Sublethal effects of bio-plastic microparticles and their components on the behaviour of Daphnia magna.

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Savva, Katerina, Farré, Marinella, Barata, Carlos·2023·Environmental Research
DOI 10.1016/j.envres.2023.116775
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Abstract

Bioplastics arise as an alternative to plastic production delinked from fossil resources. However, as their demand is increasing, there is a need to investigate their environmental fingerprint. Here we study the toxicity of microplastics (MPLs) of two widely used materials, the polylactic acid (PLA) and the polyhydroxybutyrate (PHB) on the environmental aquatic model species Daphnia magna. The study was focused on sublethal behavioural and feeding endpoints linked to antipredator scape responses and food intake. The study aimed to test that MPLs from single-use household comercial items and among them bioplastics should be more toxic than those obtained from standard plastic polymers and fossil plastic materials due to the greater amount of plastic additives, and that MPLs should be more toxic than plastic extracts due to the contribution of both particle and plastic additive toxicity. MPLs were obtained by cryogenic grinding and sea-sand erosion to obtain irregular particles. MPL included standard polymers and nine comercial items of PLA and PHB and one fossil-based material of high-density polyethylene (HDPE). The additive content in commercial items was characterised by liquid chromatography coupled with high-resolution mass spectrometry. D. magna juveniles were exposed for 24 h to particles and their plastic extracts. Results indicated that the toxicity of bioplastic particles was five times higher than the effects produced by exposure to the content of the additives alone, that bioplastic particles were more toxic than fossil ones and that particles obtained from commercial items were more toxic than those obtained from PLA, PHB or HDPE polymer standards. Predicted toxicity from the measured plastic additives in the studied commercially available household items, however, was poorly related with the observed behavioural and feeding effects. Further research on unknown chemical components together with physical factors is need it to fully understand the mechanisms of toxicity of bioplastic materials.

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Authors
Savva, K., Farré, M., & Barata, C.
Affiliations
Institute of Environmental Assessment and Water Research (ES)
Corresponding author
Barata, Carlos
ORCID
Venue
Environmental Research
Publisher
Elsevier BV
Published
2023-11
Volume
236
Issue
Pt 2
Pages
116775
ISSN
0013-9351 (ISSN-L)
DOI
10.1016/j.envres.2023.116775
OpenAlex
W4385336002
Cited by
41
References
63
Citations by year
2026: 10 · 2025: 14 · 2024: 16 · 2023: 1
FWCI
2.74
Type
journal article
Language
en
Open access
Licence
Locations
  • Environmental Research (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
  • DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion, other-oa)
Funders
  • Spanish Scientific Research Council
  • Ministerio de Ciencia e Innovación
  • Generalitat de Catalunya (2021 SGR 01150-On-Health)
Topics
  • Physical Sciences › Environmental Science › Pollution › Microplastics and Plastic Pollution
  • Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Environmental Toxicology and Ecotoxicology
  • Physical Sciences › Environmental Science › Pollution › Pharmaceutical and Antibiotic Environmental Impacts
Keywords
bioplastics, microplastics, polylactic acid, polyhydroxybutyrate, Daphnia magna, sublethal toxicity, antipredator behavior, feeding behaviour, polymer additives, high-density polyethylene, aquatic toxicity
MeSH terms
Microplastics, Polyhydroxybutyrates, Animals, Biopolymers, Daphnia, Plastics, Polyesters, Water Pollutants, Chemical, Polyethylene

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