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Oxidative stress responses of rats exposed to Roundup and its active ingredient glyphosate
Abstract
Glyphosate is the active ingredient and polyoxyethyleneamine, the major component, is the surfactant present in the herbicide Roundup formulation. The objective of this study was to analyze potential cytotoxicity of the Roundup and its fundamental substance (glyphosate). Albino male rats were intraperitoneally treated with sub-lethal concentration of Roundup (269.9mg/kg) or glyphosate (134.95mg/kg) each 2 days, during 2 weeks. Hepatotoxicity was monitored by quantitative analysis of the serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) activities, total protein, albumin, triglyceride and cholesterol. Creatinine and urea were used as the biochemical markers of kidney damages. The second aim of this study to investigate how glyphosate alone or included in herbicide Roundup affected hepatic reduced glutathione (GSH) and lipid peroxidation (LPO) levels of animals as an index of antioxidant status and oxidative stress, respectively, as well as the serum nitric oxide (NO) and alpha tumour necrosis factor (TNF-α) were measured. Treatment of animals with Roundup induced the leakage of hepatic intracellular enzymes, ALT, AST and ALP suggesting irreversible damage in hepatocytes starting from the first week. It was found that the effects were different on the enzymes in Roundup and glyphosate-treated groups. Significant time-dependent depletion of GSH levels and induction of oxidative stress in liver by the elevated levels of LPO, further confirmed the potential of Roundup to induce oxidative stress in hepatic tissue. However, glyphosate caused significant increases in NO levels more than Roundup after 2 weeks of treatment. Both treatments increased the level of TNF-α by the same manner. The results suggest that excessive antioxidant disruptor and oxidative stress is induced with Roundup than glyphosate.
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“It is thus possible that NK603 GM maize consumption, with its reduced levels of these compounds, may have provoked the early aging of the kidney physiology, similarly to R exposure causing oxidative stress [41].”
From Republished study: long-term toxicity of a Roundup herbicide and a Roundup-tolerantgenetically modified maize (2014) · cites this paper as [41]
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Sule, R. O., Condon, L., & Gomes, A. V. (2022) · Oxidative Medicine and Cellular Longevity · doi:10.1155/2022/5563759 · Cited by 614
“Adult albino male rats (liver) ↓ ↑ [181]”
Potential toxic effects of glyphosate and its commercial formulations below regulatory limits
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“(El-Shenawy, 2009) Albino (M) R/G 2 w 135 each 2 d Hepatotoxicity: b Liver/body weight ratio; For b th R and G: b lipid peroxydation, ALAT, ASAT and”
BackgroundZhang, L., Rana, I., Shaffer, R. M., Taioli, E., & Sheppard, L. A. (2019) · Mutation Research/Reviews in Mutation Research · doi:10.1016/j.mrrev.2019.02.001 · Cited by 381
The impact and toxicity of glyphosate and glyphosate-based herbicides on health and immunity
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“When rats were injected intraperitoneally with RoundUpVR or glyphosate alone on alternative days for 1 or 2 weeks, their serum TNF levels increased (El-Shenawy 2009).”
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Metadata
- Authors
- El-Shenawy, N. S.
- Venue
- Environmental Toxicology and Pharmacology
- Publisher
- Elsevier BV
- Published
- 2009-11
- Volume
- 28
- Issue
- 3
- Pages
- 379-385
- ISSN
- 1382-6689 (ISSN-L)
- OpenAlex
- W2158634663
- Cited by
- 207
- References
- 39
- Citations by year
- 2026: 16 · 2025: 10 · 2024: 13 · 2023: 16 · 2022: 24 · 2021: 18 · 2020: 18 · 2019: 24 · 2018: 9 · 2017: 12 · 2016: 11 · 2015: 3 · 2014: 16 · 2013: 11 · 2012: 4
- FWCI
- 2.43
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Environmental Toxicology and Pharmacology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Toxicology › Pesticide Exposure and Toxicity
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Toxicology › Paraquat toxicity studies and treatments
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmacology › Drug-Induced Hepatotoxicity and Protection
- Keywords
- glyphosate, Roundup, herbicide toxicity, oxidative stress, hepatotoxicity, reduced glutathione, lipid peroxidation, nitric oxide, TNF-α, antioxidant status, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, kidney injury, rats
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