The DOI matches its Crossref record; 39 of the 39 references it cites were found in a registry or an index.
Elucidation of the role of LMO2 in human erythroid cells
Open accessAbstract
LIM-only protein 2 (LMO2) is a non-DNA-binding component of a protein complex containing master regulators of hematopoiesis, including GATA-1, SCL/TAL1, and LDB1. However, the role of LMO2 in human erythroid differentiation is unclear. LMO2 knockdown in hemin-treated K562 cells reduced the benzidine-positive cell ratio, suggesting that LMO2 retards hemin-mediated K562 cell differentiation. Microarray analysis using K562 cells after siRNA-mediated LMO2 knockdown indicated that 177 and 78 genes were upregulated and downregulated (>1.5-fold), respectively. The downregulated gene ensemble contained prototypical erythroid genes (HBB, SLC4A1). Whereas LMO2 knockdown did not affect GATA-1 or SCL/TAL1 expression, it resulted in significantly reduced chromatin occupancy of GATA-1, SCL/TAL1, and LDB1 at the β-globin locus control region and SLC4A1 locus in both K562 cells and human induced pluripotent stem cell-derived erythroid cells. Introduction of GATA-1 mutations, shown to impair direct interaction with LMO2, significantly diminished chromatin occupancy. On the other hand, knockdown of either SCL/TAL1 or LDB1 also resulted in significantly reduced chromatin occupancy of GATA-1 at endogenous loci, suggesting that impaired assembly of these components also affects GATA-1 chromatin occupancy. In an ex vivo model of erythroid differentiation from CD34(+) cells, LMO2 protein level peaked on day 5 and decreased at later stages of differentiation. The LMO2 expression pattern was similar to those of GATA-1 and SCL/TAL1. Furthermore, shRNA-mediated LMO2 knockdown in primary erythroblasts suggested that LMO2 regulates HBB, HBA, and SLC4A1 expression. LMO2 contributes to GATA-1 target gene expression by affecting assembly of the GATA-SCL/TAL1 complex components at endogenous loci.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“However, simulation results using Paul et al. data16 showed a different phenotype in erythrocyte cells, suggesting that Lmo2 is also crucial for promoting erythroid differentiation (Extended Data Fig. 6d).”
From Dissecting cell identity via network inference and in silico gene perturbation (2023) · cites this paper as [75]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Morgenstern, J. P., & Land, H. (1990) · Nucleic Acids Research · doi:10.1093/nar/18.12.3587 · Cited by 2,099
An early haematopoietic defect in mice lacking the transcription factor GATA-2
Tsai, F., Keller, G. M., Kuo, F. C., Weiss, M. J., Chen, J., Rosenblatt, M., Alt, F. W., & Orkin, S. H. (1994) · Nature · doi:10.1038/371221a0 · Cited by 1,447
Absence of blood formation in mice lacking the T-cell leukaemia oncoprotein tal-1/SCL
Shivdasani, R. A., Mayer, E. L., & Orkin, S. H. (1995) · Nature · doi:10.1038/373432a0 · Cited by 938
Human Fetal Hemoglobin Expression Is Regulated by the Developmental Stage-Specific Repressor BCL11A
Sankaran, V. G., Menne, T. F., Xu, J., Akie, T. E., Lettre, G., Van Handel, B., Mikkola, H. K. A., Hirschhorn, J. N., Cantor, A. B., & Orkin, S. H. (2008) · Science · doi:10.1126/science.1165409 · Cited by 919
Wadman, I., Osada, H., Grütz, G. G., Agulnick, A. D., Westphal, H., Forster, A., & Rabbitts, T. H. (1997) · The EMBO Journal · doi:10.1093/emboj/16.11.3145 · Cited by 836
The Oncogenic Cysteine-rich LIM domain protein Rbtn2 is essential for erythroid development
Warren, A. J., Colledge, W. H., Carlton, M. B. L., Evans, M. J., Smith, A. J. H., & Rabbitts, T. H. (1994) · Cell · doi:10.1016/0092-8674(94)90571-1 · Cited by 611
Activity and tissue-specific expression of the transcription factor NF-E1 multigene family.Preprint
Yamamoto, M. P., Ko, L. J., Leonard, M. W., Beug, H., Orkin, S. H., & Engel, J. D. (1990) · Genes & Development · doi:10.1101/gad.4.10.1650 · Cited by 598
Evans, T., Reitman, M. L., & Felsenfeld, G. (1988) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.85.16.5976 · Cited by 485
Kurita, R., Suda, N., Sudo, K., Miharada, K., Hiroyama, T., Miyoshi, H., Tani, K., & Nakamura, Y. (2013) · PLoS ONE · doi:10.1371/journal.pone.0059890 · Cited by 418
Boehm, T. L. J., Foroni, L., Kaneko, Y., Perutz, M. F., & Rabbitts, T. H. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.10.4367 · Cited by 373
Cited by
Sorted by citations
SCENIC+: single-cell multiomic inference of enhancers and gene regulatory networks
Bravo González-Blas, C., De Winter, S., Hulselmans, G., Hecker, N., Matetovici, I., Christiaens, V., Poovathingal, S., Wouters, J., Aibar, S., & Aerts, S. (2023) · Nature Methods · doi:10.1038/s41592-023-01938-4 · Cited by 778
Dissecting cell identity via network inference and in silico gene perturbation
Kamimoto, K., Stringa, B., Hoffmann, C. M., Jindal, K., Solnica-Krezel, L., & Morris, S. A. (2023) · Nature · doi:10.1038/s41586-022-05688-9 · Cited by 677
SCENIC+: single-cell multiomic inference of enhancers and gene regulatory networks
González-Blas, C. B., De Winter, S., Hulselmans, G., Hecker, N., Matetovici, I., Christiaens, V., Poovathingal, S., Wouters, J., Aibar, S., & Aerts, S. (2022) · doi:10.1101/2022.08.19.504505 · Cited by 778
Metadata
- Authors
- Inoue, A., Fujiwara, T., Okitsu, Y., Katsuoka, Y., Fukuhara, N., Onishi, Y., Ishizawa, K., & Harigae, H.
- Affiliations
- Tohoku University (JP)
- Corresponding author
- Fujiwara, Tohru
- Venue
- Experimental Hematology
- Publisher
- Elsevier BV
- Published
- 2013-12
- Volume
- 41
- Issue
- 12
- Pages
- 1062-1076.e1
- ISSN
- 0301-472X (ISSN-L)
- OpenAlex
- W1983456610
- Cited by
- 15
- References
- 39
- Citations by year
- 2026: 3 · 2024: 1 · 2023: 2 · 2022: 1 · 2021: 3 · 2020: 1 · 2019: 1 · 2017: 3
- FWCI
- 0.00
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Experimental Hematology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Japan Society for the Promotion of Science (23791067)
- RIKEN
- Topics
- Health Sciences › Medicine › Genetics (clinical) › Hemoglobinopathies and Related Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
- Keywords
- LMO2, erythroid differentiation, GATA-1, SCL/TAL1, LDB1, chromatin occupancy, beta-globin locus control region, band 3, hemin, K562 cells, erythroblasts, gene expression regulation
- MeSH terms
- Cell Differentiation, Erythroblasts, Flow Cytometry, Gene Expression Regulation, Hemin, Humans, Proto-Oncogene Proteins, Blotting, Western, Gene Expression Profiling, Erythroid Cells, Cell Line, Tumor, Adaptor Proteins, Signal Transducing, Gene Knockdown Techniques, LIM Domain Proteins, Real-Time Polymerase Chain Reaction
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Elucidation of the role of LMO2 in human erythroid cells. https://app.cochat.ai/papers/10.1016/j.exphem.2013.09.003
- The paper
- Inoue, A., Fujiwara, T., Okitsu, Y., Katsuoka, Y., Fukuhara, N., Onishi, Y., Ishizawa, K., & Harigae, H. (2013). Elucidation of the role of LMO2 in human erythroid cells. Experimental Hematology. https://doi.org/10.1016/j.exphem.2013.09.003
