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Neurogenesis in Tα-1 tubulin transgenic mice during development and after injury

Coksaygan, Turhan, Magnus, Tim, Cai, Jingli, Mughal, Mohammed, Lepore, Angelo, Xue, Haipeng, Fischer, Itzhac, Rao, Mahendra S.·2006·Experimental Neurology
DOI 10.1016/j.expneurol.2005.10.030
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Abstract

Talpha-1 tubulin promoter-driven EYFP expression is seen in murine neurons born as early as E9.5. Double labeling with markers for stem cells (Sox 1, Sox 2, nestin), glial progenitors (S100beta, NG2, Olig2), and neuronal progenitors (doublecortin, betaIII-tubulin, PSA-NCAM) show that Talpha-1 tubulin expression is limited to early born neurons. BrdU uptake and double labeling with neuronal progenitor markers in vivo and in vitro show that EYFP-expressing cells are postmitotic and Talpha-1 tubulin EYFP precedes the expression of MAP-2 and NeuN, and follows the expression of PSA-NCAM, doublecortin (Dcx), and betaIII-tubulin. Talpha-1 tubulin promoter-driven EYFP expression is transient and disappears in most neurons by P0. Persistent EYFP expression is mainly limited to scattered cells in the subventricular zone (SVZ), rostral migratory stream, and hippocampus. However, there are some areas that continue to express Talpha-1 tubulin in the adult without apparent neurogenesis. The number of EYFP-expressing cells declines with age indicating that Talpha-1 tubulin accurately identifies early born postmitotic neurons throughout development but less clearly in the adult. Assessment of neurogenesis after stab wound injuries in the cortex, cerebellum and spinal cord of adult animals shows no neurogenesis in most areas with an increase in BrdU incorporation in glial and other non neuronal populations. An up-regulation of Talpha-1 tubulin can be seen in certain areas unaccompanied by new…

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Metadata

Authors
Coksaygan, T., Magnus, T., Cai, J., Mughal, M., Lepore, A., Xue, H., Fischer, I., & Rao, M. S.
Venue
Experimental Neurology
Publisher
Elsevier BV
Published
2006-02
Volume
197
Issue
2
Pages
475-485
ISSN
0014-4886
DOI
10.1016/j.expneurol.2005.10.030
Cited by
34
References
0
Language
en
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)

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