Toxicity reduction of ochratoxin A by lactic acid bacteria
Abstract
Ochratoxin A (OTA) is a mycotoxin produced by the metabolism of fungus belonging to the genus Aspergillus and Penicillium. In this paper we report, the capacity of different cultures of lactic acid bacteria (LAB) to degrade OTA present in MRS broth at both pH 3.5 and 6.5. A study of OTA reduction during gastrointestinal digestion carried out with the LAB was also performed. Taking into account the two reduction mechanisms of OTA studied in this work as the enzymatic one and the adsorption on the cell wall, as well as at pH 3.5 and 6.5 the reduction values of OTA were in a range of 30-99%, being the strains with greater reduction (97% and 95%) Lb. rhamnosus CECT 278T and Lb. plantarum CECT 749 respectively. In the experiments carried out digesting the OTA in MRS medium with LAB, the highest bioaccessibility reduction was observed by the strain of Lb. johnsonii CECT 289, showing a mean reduction around all the gastrointestinal digestion process of 97.4%. The mass spectrometry associated to the linear ion trap method identified ochratoxin alpha (OTα) m/z = 256.1 and phenylalanine (Phe) m/z = 166.1 as the major metabolites of OTA degradation in LAB cultures.
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Strains with the highest OTA biodegradation were claimed to be usable in the silage industry as inoculants or feed additives to reduce OTA intake in animal production, considering animals are the most susceptible livestock to OTA toxicity.
“The strains that evidenced the highest OTA biodegradation were claimed to be used in the silage industry as inoculants or as feed additives to reduce the problem of the intake of OTA in animal production, considering these animals are the most susceptible livestock to the toxic effects of OTA.”
From Trends in designing microbial silage quality by biotechnological methods using lactic acid bacteria inoculants: a minireview. (2019) · cites this paper as Luz et al. 2018
Strains characterized by the same sensitivity to mycotoxins may differ significantly in the degree of degradation in the environment.
“Strains that are characterized by the same sensitivity to mycotoxins may differ significantly in the degree of its degradation in the environment (Luz et al. 2018).”
From Trends in designing microbial silage quality by biotechnological methods using lactic acid bacteria inoculants: a minireview. (2019) · cites this paper as Luz et al. 2018
Luz et al. studied the influence of some Bifidobacterium spp. and Lactobacillus spp. on OTA reduction in medium by adsorption to the cell wall and hydrolytic degradation by bacterial proteases.
“Recently, Luz et al. (2018) studied the influence of some Bifidobacterium spp. and Lactobacillus spp. on OTA reduction in medium by adsorption to the cell wall, as well as hydrolytic degradation of mycotoxin by bacterial proteases.”
From Trends in designing microbial silage quality by biotechnological methods using lactic acid bacteria inoculants: a minireview. (2019) · cites this paper as Luz et al. 2018
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Lactic acid bacteria as antifungal and anti-mycotoxigenic agents: A comprehensive review
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- Authors
- Luz, C., Ferrer, J., Mañes, J., & Meca, G.
- Affiliations
- Universitat de València (ES)
- Corresponding author
- Meca, G.
- Venue
- Food and Chemical Toxicology
- Publisher
- Elsevier BV
- Published
- 2018-02
- Volume
- 112
- Pages
- 60-66
- ISSN
- 0278-6915 (ISSN-L)
- OpenAlex
- W2778798265
- Cited by
- 106
- References
- 24
- Citations by year
- 2026: 9 · 2025: 13 · 2024: 10 · 2023: 13 · 2022: 27 · 2021: 8 · 2020: 11 · 2019: 13 · 2018: 2
- FWCI
- 7.00
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Food and Chemical Toxicology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Economy and Competitiveness (AGL2016-77610R)
- Generalitat Valenciana (GV-2016-106)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Mycotoxins in Agriculture and Food
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Phytase and its Applications
- Keywords
- ochratoxin A, mycotoxin detoxification, lactic acid bacteria, ochratoxin alpha, enzymatic degradation, cell wall adsorption, gastrointestinal digestion, bioaccessibility, Lactobacillus rhamnosus, Lactobacillus plantarum, Lactobacillus johnsonii, phenylalanine, mycotoxin metabolites
- MeSH terms
- Adsorption, Cell Wall, Chromatography, Liquid, Culture Media, Food Contamination, Humans, Inactivation, Metabolic, Ochratoxins, Phenylalanine, Reference Standards, Mass Spectrometry, Gastrointestinal Tract, Lactobacillales
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