Cells synchronized in S phase show increased rate of repair of UV damaged plasmids
Open accessAbstract
The capacity for nucleotide excision repair of cells synchronized in S phase and unsynchronized cells was compared by the host cell reactivation assay and the cell‐free repair system. HeLa cells were transfected with in vitro damaged by UV irradiation pEGFP and the repair capacity was determined by the number of fluorescent cells. In the cell‐free repair system, the repair capacity of protein extracts isolated from K562 cells was determined by measuring the transformation efficiency of UV irradiated pBlueScript incubated in the extracts. In both cases, the repair capacity of the cells synchronized in S phase cells was 30–50% higher than the repair capacity of unsynchronized cells.
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In mammalian cells, NER also acts in S phase, where GG-NER is enhanced and stimulated by active ATR.
“In mammalian cells the situation is more complex, because NER acts also in S phase, where GG-NER is enhanced [88] and is stimulated by active ATR [89,90].”
From Mind the gap: keeping UV lesions in check (2011) · cites this paper as [88]
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“In mammalian cells the situation is more complex, because NER cts also in S phase, where GG-NER is enhanced [88] and is stimuated by active ATR [89,90].”
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- Authors
- Gospodinov, A., & Anachkova, B.
- Affiliations
- Bulgarian Academy of Sciences (BG)
- Corresponding author
- Anachkova, Boyka
- ORCID
- Gospodinov, Anastas
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 2004-07-23
- Volume
- 572
- Issue
- 1-3
- Pages
- 99-102
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W2076859432
- Cited by
- 3
- References
- 24
- Citations by year
- 2014: 1
- FWCI
- 0.00
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- FEBS Letters (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Foundation
- Wellcome Trust
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Carcinogens and Genotoxicity Assessment
- Keywords
- nucleotide excision repair, S phase synchronization, UV-induced DNA damage, host cell reactivation assay, HeLa cells, K562 cells, DNA repair capacity
- MeSH terms
- Cell Cycle, Cell Line, DNA Repair, DNA, Neoplasm, Humans, Kinetics, Plasmids, Ultraviolet Rays, Xeroderma Pigmentosum, S Phase, K562 Cells
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