Cited by 75Polysaccharides and Plant Cell WallsPlant nutrient uptake and metabolismPlant Molecular Biology Research

In Arabidopsis thaliana, the invertase inhibitors AtC/VIF1 and 2 exhibit distinct target enzyme specificities and expression profiles

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Link, Manuela, Rausch, Thomas, Greiner, Steffen·2004·FEBS Letters

Abstract

Plant cell wall (CWI) and vacuolar invertases (VI) play important roles in carbohydrate metabolism, stress responses and sugar signaling. Addressing the regulation of invertase activities by inhibitor proteins (C/VIF, cell wall/vacuolar inhibitor of fructosidase), we have identified two C/VIFs from Arabidopsis thaliana. AtC/VIF1 showed specific inhibition of VI activity, whereas AtC/VIF2 inhibited both, CWI and VI. Expression analysis revealed that expression of AtC/VIF1 was restricted to specific organs, AtC/VIF2, however, was weakly expressed throughout plant development. Promoter::GUS transformants confirmed pronounced differences of tissue/cell type-specific expression between both isoforms. Growth of an AtC/VIF1 T-DNA KO mutant was unaffected, but VI activity and hexose content were slightly increased.

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Crossref · 10.1016/j.febslet.2004.07.062

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Authors
Link, M., Rausch, T., & Greiner, S.
Corresponding author
Greiner, Steffen
Venue
FEBS Letters
Publisher
Wiley
Published
2004-08-03
Volume
573
Issue
1-3
Pages
105-109
ISSN
  • 0014-5793 (ISSN-L)
  • 1873-3468
OpenAlex
W2015790535
Cited by
75
References
33
Citations by year
2026: 2 · 2025: 1 · 2024: 1 · 2023: 1 · 2022: 5 · 2021: 4 · 2020: 10 · 2019: 5 · 2018: 6 · 2017: 4 · 2016: 4 · 2015: 6 · 2014: 2 · 2013: 3 · 2012: 1
FWCI
3.53
Type
journal article
Language
en
Open access
Locations
  • FEBS Letters (publishedVersion)
  • PubMed (publishedVersion)
Funders
European Molecular Biology Laboratory
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Polysaccharides and Plant Cell Walls
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant nutrient uptake and metabolism
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
Keywords
invertase inhibitor, vacuolar invertase, cell wall invertase, Arabidopsis thaliana, enzyme inhibition specificity, tissue-specific gene expression, knockout mutants, hexose content, carbohydrate metabolism, sugar signaling
MeSH terms
DNA, Bacterial, Enzyme Inhibitors, Hexoses, Organ Specificity, Promoter Regions, Genetic, Substrate Specificity, Polymerase Chain Reaction, Gene Deletion, Arabidopsis, Gene Expression Regulation, Plant, Plant Leaves, RNA, Plant, Protein Isoforms, Gene Expression Profiling, Arabidopsis Proteins, beta-Fructofuranosidase

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