In Arabidopsis thaliana, the invertase inhibitors AtC/VIF1 and 2 exhibit distinct target enzyme specificities and expression profiles
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Plant cell wall (CWI) and vacuolar invertases (VI) play important roles in carbohydrate metabolism, stress responses and sugar signaling. Addressing the regulation of invertase activities by inhibitor proteins (C/VIF, cell wall/vacuolar inhibitor of fructosidase), we have identified two C/VIFs from Arabidopsis thaliana. AtC/VIF1 showed specific inhibition of VI activity, whereas AtC/VIF2 inhibited both, CWI and VI. Expression analysis revealed that expression of AtC/VIF1 was restricted to specific organs, AtC/VIF2, however, was weakly expressed throughout plant development. Promoter::GUS transformants confirmed pronounced differences of tissue/cell type-specific expression between both isoforms. Growth of an AtC/VIF1 T-DNA KO mutant was unaffected, but VI activity and hexose content were slightly increased.
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INVinh has been reported to mediate the post-translational regulation of invertase, and the activity of one invertase might be regulated by several INVinhs.
“However, INVinh has been reported to mediate the post-translational regulation of invertase, and the activity of one invertase might be regulated by several INVinhs (Link et al., 2004; Lin et al., 2013).”
From Comparative Transcriptome Analysis Reveals Critical Function of Sucrose Metabolism Related-Enzymes in Starch Accumulation in the Storage Root of Sweet Potato (2017) · cites this paper as Link et al., 2004
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- Authors
- Link, M., Rausch, T., & Greiner, S.
- Corresponding author
- Greiner, Steffen
- ORCID
- Rausch, Thomas
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 2004-08-03
- Volume
- 573
- Issue
- 1-3
- Pages
- 105-109
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W2015790535
- Cited by
- 75
- References
- 33
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 1 · 2023: 1 · 2022: 5 · 2021: 4 · 2020: 10 · 2019: 5 · 2018: 6 · 2017: 4 · 2016: 4 · 2015: 6 · 2014: 2 · 2013: 3 · 2012: 1
- FWCI
- 3.53
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- FEBS Letters (publishedVersion)
- PubMed (publishedVersion)
- Funders
- European Molecular Biology Laboratory
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Polysaccharides and Plant Cell Walls
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant nutrient uptake and metabolism
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Keywords
- invertase inhibitor, vacuolar invertase, cell wall invertase, Arabidopsis thaliana, enzyme inhibition specificity, tissue-specific gene expression, knockout mutants, hexose content, carbohydrate metabolism, sugar signaling
- MeSH terms
- DNA, Bacterial, Enzyme Inhibitors, Hexoses, Organ Specificity, Promoter Regions, Genetic, Substrate Specificity, Polymerase Chain Reaction, Gene Deletion, Arabidopsis, Gene Expression Regulation, Plant, Plant Leaves, RNA, Plant, Protein Isoforms, Gene Expression Profiling, Arabidopsis Proteins, beta-Fructofuranosidase
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