Dicing and slicing: The core machinery of the RNA interference pathway
Abstract
RNA interference (RNAi) is broadly defined as a gene silencing pathway that is triggered by double‐stranded RNA (dsRNA). Many variations have been described on this theme. The dsRNA trigger can be supplied exogenously, as an experimental tool, or can derive from the genome in the form of microRNAs. Gene silencing can be the result of nucleolytic degradation of the mRNA, or by translational suppression. At the heart of the pathway are two ribonuclease machines. The ribonuclease III enzyme Dicer initiates the RNAi pathway by generating the active short interfering RNA trigger. Silencing is effected by the RNA‐induced silencing complex and its RNaseH core enzyme Argonaute. This review describes the discovery of these machines and discusses future lines of work on this amazing biochemical pathway.
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Catalytically functional miRNAs must be loaded into the miRNA-processing protein Ago2.
“Because catalytically functional miRNAs must be loaded into the miRNA-processing protein Ago2 (Hammond et al., 2001;Hammond, 2005)”
From MicroRNA Profiling Reveals Marker of Motor Neuron Disease in ALS Models (2017) · cites this paper as Hammond, 2005
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The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14
Lee, R. C., Feinbaum, R. L., & Ambros, V. (1993) · Cell · doi:10.1016/0092-8674(93)90529-y · Cited by 12,948 · see citing works
Wightman, B., Ha, I., & Ruvkun, G. (1993) · Cell · doi:10.1016/0092-8674(93)90530-4 · Cited by 4,292 · see citing works
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Makarova, K. S., Grishin, N. V., Shabalina, S. A., Wolf, Y. I., & Koonin, E. V. (2006) · Biology Direct · doi:10.1186/1745-6150-1-7 · Cited by 1,199 · see citing works
MicroRNA Profiling Reveals Marker of Motor Neuron Disease in ALS Models
Hoye, M. L., Koval, E. D., Wegener, A. J., Hyman, T. S., Yang, C., O'Brien, D. R., Miller, R. L., Cole, T., Schoch, K. M., Shen, T., et al. (2017) · The Journal of Neuroscience · doi:10.1523/jneurosci.3582-16.2017 · Cited by 82 · see citing works
Dicer ablation in Kiss1 neurons impairs puberty and fertility preferentially in female mice
Roa, J., Ruiz-Cruz, M., Ruiz-Pino, F., Onieva, R., Vazquez, M. J., Sanchez-Tapia, M. J., Ruiz-Rodriguez, J. M., Sobrino, V., Barroso, A., Heras, V., et al. (2022) · Nature Communications · doi:10.1038/s41467-022-32347-4 · Cited by 22 · see citing works
Silencing of c-myc gene expression using enzymatically and chemically synthesized siRNAs
Kabilova, T. O., Vladimirova, A. V., Repkova, M. N., Ven’yaminova, A. G., Chernolovskaya, E. L., & Vlasov, V. V. (2006) · Molecular Biology · doi:10.1134/s0026893306060136 · Cited by 5 · see citing works
- Authors
- Hammond, S. M.
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 2005-09-27
- Volume
- 579
- Issue
- 26
- Pages
- 5822-5829
- ISSN
- 0014-5793
- 1873-3468
- Cited by
- 439
- References
- 2
- Language
- en
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