Silencing of c-myc gene expression using enzymatically and chemically synthesized siRNAs
Abstract
Small interfering RNAs (siRNA) provide a powerful approach for sequence-specific silencing of gene expression. In the present study we investigated inhibition of c-myc gene expression by siRNAs targeted to the sequence 1452-1470 b. in third exon of c-myc mRNA and to homologous regions in second exons of c-myc (697-715 b.) and N-myc (302-320 b.) mRNAs. siRNAs were prepared enzymatically according to the scheme, including dsDNA-templates preparation using Klenow fragment, separate in vitro transcription of each RNA strand with subsequent hybridization and removal of leader sequences by T1 RNase. Investigation of c-myc gene silencing by siRNAs revealed that enzymatically prepared siRNAs induce stronger inhibition of c-myc expression, than siRNA with the same sequence prepared by chemical synthesis. It was found that down-regulation of c-myc gene expression by investigated siRNAs results in efficient inhibition and even complete arrest of carcinoma cell proliferation, moreover, the extend of growth inhibition correlates with the level of siRNA-mediated reduction of c-myc mRNA.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Kabilova and colleagues synthesized siRNAs targeting different specific regions of c-Myc mRNA that reduced c-Myc mRNA levels in KB-3-1 human epidermoid carcinoma cells and inhibited cell proliferation.
“In 2006, Kabilova and colleagues synthesized siRNAs, compound (241), targeting different specific regions of the c-Myc mRNA using both enzymatic and chemical methods. Both types of synthesized siRNAs were effective in reducing c-Myc mRNA levels in KB-3-1 human epidermoid carcinoma cells, leading to inhibition of cell proliferation.575”
From Recent advances in targeting the “undruggable” proteins: from drug discovery to clinical trials (2023) · cites this paper as [575]
Cites
Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
Fire, A., Xu, S., Montgomery, M. K., Kostas, S. A., Driver, S. E., & Mello, C. C. (1998) · Nature · doi:10.1038/35888 · Cited by 15,429 · see citing works
Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
Elbashir, S. M., Harborth, J., Lendeckel, W., Yalcin, A., Weber, K., & Tuschl, T. (2001) · Nature · doi:10.1038/35078107 · Cited by 9,456 · see citing works
Carmichael, J., DeGraff, W., Gazdar, A. F., Minna, J. D., & Mitchell, J. B. (1987) · PubMed · Cited by 3,813 · see citing works
Mechanisms of gene silencing by double-stranded RNA
Meister, G., & Tuschl, T. (2004) · Nature · doi:10.1038/nature02873 · Cited by 2,539 · see citing works
Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templates.
Milligan, J., Groebe, D., Witherell, G. W., & Uhlenbeck, O. (1987) · Nucleic Acids Research · doi:10.1093/nar/15.21.8783 · Cited by 1,971 · see citing works
Mapping adenines, guanines, and pyrimidines in RNA
Donis-Keller, H., Maxam, A. M., & Gilbert, W. (1977) · Nucleic Acids Research · doi:10.1093/nar/4.8.2527 · Cited by 1,380 · see citing works
Ui‐Tei, K. (2004) · Nucleic Acids Research · doi:10.1093/nar/gkh247 · Cited by 810 · see citing works
THE myc ONCOGENE: ITS ROLE IN TRANSFORMATION AND DIFFERENTIATION
Cole, M. D. (1986) · Annual Review of Genetics · doi:10.1146/annurev.ge.20.120186.002045 · Cited by 695 · see citing works
Tolerance for mutations and chemical modifications in a siRNA
Amarzguioui, M. (2003) · Nucleic Acids Research · doi:10.1093/nar/gkg147 · Cited by 534 · see citing works
RNA Interference in Mammalian Cells by Chemically-Modified RNA
Braasch, D. A., Jensen, S., Liu, Y., Kaur, K. J., Arar, K., White, M. A., & Corey, D. R. (2003) · Biochemistry · doi:10.1021/bi0343774 · Cited by 531 · see citing works
Extreme instability of myc mRNA in normal and transformed human cells.
Dani, C., Blanchard, J., Piechaczyk, M., SABOUTY, S. E., Marty, L., & Jeanteur, P. (1984) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.81.22.7046 · Cited by 517 · see citing works
Proteins encoded by the human c-myc oncogene: differential expression in neoplastic cells.
Hann, S., & Eisenman, R. N. (1984) · Molecular and Cellular Biology · doi:10.1128/mcb.4.11.2486 · Cited by 503 · see citing works
Dicing and slicing: The core machinery of the RNA interference pathway
Hammond, S. M. (2005) · FEBS Letters · doi:10.1016/j.febslet.2005.08.079 · Cited by 439 · see citing works
[9] Specific labeling of 3′ termini of RNA with T4 RNA ligase
England, T. E., Bruce, A. G., & Uhlenbeck, O. C. (1980) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(80)65011-3 · Cited by 420 · see citing works
Interferon induction by siRNAs and ssRNAs synthesized by phage polymerase
Kim, D.-H., Longo, M., Han, Y. S., Lundberg, P. S., Cantin, E. M., & Rossi, J. J. (2004) · Nature Biotechnology · doi:10.1038/nbt940 · Cited by 382 · see citing works
RNA interference in mammalian cells using siRNAs synthesized with T7 RNA polymerase
Donzé, O. (2002) · Nucleic Acids Research · doi:10.1093/nar/30.10.e46 · Cited by 275 · see citing works
Ensembl 2002: accommodating comparative genomics
Clamp, M. E. (2003) · Nucleic Acids Research · doi:10.1093/nar/gkg083 · Cited by 243 · see citing works
myc Family Oncogenes in the Development of Normal and Neoplastic Cells
DePinho, R. A., Schreiber‐Agus, N., & Alt, F. W. (1991) · Advances in cancer research · doi:10.1016/s0065-230x(08)60994-x · Cited by 229 · see citing works
Approaches for chemically synthesized siRNA and vector‐mediated RNAi
Amarzguioui, M., Rossi, J. J., & Kim, D.-H. (2005) · FEBS Letters · doi:10.1016/j.febslet.2005.08.070 · Cited by 222 · see citing works
Therapeutic Potential of RNA Interference
Stevenson, M. (2004) · New England Journal of Medicine · doi:10.1056/nejmra045004 · Cited by 212 · see citing works
The protein encoded by the human proto-oncogene c-myc.
Ramsay, G. M., Evan, G. I., & Bishop, J. M. (1984) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.81.24.7742 · Cited by 195 · see citing works
Enhancement of RNAi activity by improved siRNA duplexes
Hohjoh, H. (2003) · FEBS Letters · doi:10.1016/s0014-5793(03)01492-3 · Cited by 87 · see citing works
Oligoribonucleotide Synthesis: The Silyl-Phosphoramidite Method
Damha, M. J., & Ogilvie, K. K. (2003) · Humana Press eBooks · doi:10.1385/0-89603-281-7:81 · Cited by 87 · see citing works
A simple and cost-effective method for producing small interfering RNAs with high efficacy
Sohail, M. (2003) · Nucleic Acids Research · doi:10.1093/nar/gng038 · Cited by 73 · see citing works
Inexpensive SDS/phenol method for RNA extraction from tissues.
Chattopadhyay, N., Kher, R., & Godbole, M. M. (1993) · PubMed · Cited by 60 · see citing works
Gao, X., Wang, H., & Sairenji, T. (2004) · Journal of Virology · doi:10.1128/jvi.78.21.11798-11806.2004 · Cited by 40 · see citing works
c-Myc Controls Proliferation Versus Differentiation in Human Pancreatic Endocrine Cells
Demeterco, C., Itkin‐Ansari, P., Tyrberg, B., Ford, L. P., Jarvis, R. A., & Levine, F. (2002) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem.87.7.8700 · Cited by 37 · see citing works
Inhibition of EGFP expression by siRNA in EGFP-stably expressing Huh-7 cells
Cao, M., Ren, H., Pan, X., Pan, W. X., & Qi, Z. (2004) · Journal of Virological Methods · doi:10.1016/j.jviromet.2004.03.005 · Cited by 25 · see citing works
Inhibition of Human Carcinoma and Neuroblastoma Cell Proliferation by Anti-c- myc siRNA
Kabilova, T. O., Chernolovskaya, E. L., Vladimirova, A. V., & Vlassov, V. V. (2006) · Oligonucleotides · doi:10.1089/oli.2006.16.15 · Cited by 18 · see citing works
Self-citationSilencing of c‐myc Expression in Tumor Cells by siRNA
Kabilova, T. O., Chernolovskaya, E. L., Vladimirova, A. V., & Vlassov, V. V. (2004) · Nucleosides Nucleotides & Nucleic Acids · doi:10.1081/ncn-200026033 · Cited by 9 · see citing works
Self-citationRNA-Based Drugs: From RNA Interference to Short Interfering RNAs
Poliseno, L., Mercatanti, A., Citti, L., & Rainaldi, G. (2004) · Current Pharmaceutical Biotechnology · doi:10.2174/1389201043376797 · Cited by 7 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Recent advances in targeting the “undruggable” proteins: from drug discovery to clinical trials
Xie, X., Yu, T., Li, X., Zhang, N., Foster, L. J., Peng, C., Huang, W., & He, G. (2023) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-023-01589-z · Cited by 396 · see citing works
Short Double-Stranded RNA with Immunostimulatory Activity: Sequence Dependence
Kabilova, T. O., Meschaninova, M. I., Venyaminova, A. G., Nikolin, V. P., Аркадьевна, З. М., Vlassov, V. V., & Chernolovskaya, E. L. (2012) · Nucleic Acid Therapeutics · doi:10.1089/nat.2011.0328 · Cited by 35 · see citing works
Self-citationInhibition of Human Cancer-Cell Proliferation by Long Double-Stranded RNAs
Akimov, I. A., Kabilova, T. O., Vlassov, V. V., & Chernolovskaya, E. L. (2009) · Oligonucleotides · doi:10.1089/oli.2008.0151 · Cited by 7 · see citing works
Self-citationWang, C., Wang, C., Xiao, C., Zhang, W., Guo, Y. H., Qu, M., Song, Q., Qi, X., & Zou, B. (2025) · Small · doi:10.1002/smll.202410146 · Cited by 1 · see citing works
Huang, D., Zhao, H., Ming, Y., & Xiong, C. (2010) · Chin J Reprod Contracep · doi:10.1016/s1001-7844(10)60015-6
- Authors
- Kabilova, T. O., Vladimirova, A. V., Repkova, M. N., Ven’yaminova, A. G., Chernolovskaya, E. L., & Vlasov, V. V.
- Affiliations
- Russian Academy of Sciences (RU)
- Corresponding author
- Kabilova, T. O.
- Venue
- Molecular Biology
- Publisher
- Pleiades Publishing Ltd
- Published
- 2006-12
- Volume
- 40
- Issue
- 6
- Pages
- 936-944
- ISSN
- 0026-8933 (ISSN-L)
- 1608-3245
- OpenAlex
- W2092449685
- Cited by
- 5
- References
- 31
- Citations by year
- 2025: 1 · 2023: 1 · 2012: 1
- FWCI
- 0.14
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Molecular Biology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Russian Foundation for Basic Research
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Keywords
- c-Myc, siRNA, gene silencing, siRNA synthesis, chemical synthesis comparison, MYCN, Klenow fragment, mRNA down-regulation
- MeSH terms
- Base Sequence, Humans, Molecular Sequence Data, Gene Expression, Genes, myc, Proto-Oncogene Proteins c-myc, RNA, Small Interfering, Cell Line, Tumor, Cell Proliferation
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
