Cited by 10Alzheimer's disease research and treatmentsUbiquitin and proteasome pathwaysNF-κB Signaling Pathways

A ubiquitin‐binding CUE domain in presenilin‐1 enables interaction with K63‐linked polyubiquitin chains

Open access
Duggan, Stephen P., Yan, Run, McCarthy, Justin V.·2015·FEBS Letters

Abstract

The presenilins (PS1 and PS2) are the catalytic component of the γ‐secretase intramembrane protease complex, involved in the regulated intramembrane proteolysis of numerous type I transmembrane proteins, including amyloid precursor protein (APP) and Notch. Herein, we describe the identification and characterization of a CUE (coupling of ubiquitin conjugation to endoplasmic reticulum degradation) ubiquitin‐binding domain (UBD) in PS1, and demonstrate that the CUE domain of PS1 mediates non‐covalent binding to Lysine 63‐linked polyubiquitin chains. Our results highlight a γ‐secretase‐independent function for non‐covalent ubiquitin signaling in the regulation of PS1, and add new insights into the structure and function of the presenilin proteins.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1016/j.febslet.2015.03.008

Cites

48 of 48 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Research works printed but not linked: 2

  1. [19][19] [Shih, S.C., Prag, G., Francis, S.A., Sutanto, M.A., Hurley, J.H. and Hicke, L. (2003) A ubiquitin-binding motif required for intramolecular monoubiquitylation, the CUE domain. EMBO J. 22, 1273-1281.](http://refhub.elsevier.com/S0014-5793(15)00156-8/h0095) -
  2. [36][36] [Elzinga, B.M., Twomey, C., Powell, J.C., Harte, F. and McCarthy, J.V. (2009) Interleukin-1 receptor type 1 is a substrate for gamma-secretase-dependent regulated intramembrane proteolysis. J. Biol. Chem. 284, 1394-1409.](http://refhub.elsevier.com/S0014-5793(15)00156-8/h0180) -

Cited by

Sorted by citations

Authors
Duggan, S. P., Yan, R., & McCarthy, J. V.
Affiliations
University College Cork (IE)
Corresponding author
McCarthy, Justin V.
Venue
FEBS Letters
Publisher
Wiley
Published
2015-03-18
Volume
589
Issue
9
Pages
1001-1008
ISSN
  • 0014-5793 (ISSN-L)
  • 1873-3468
OpenAlex
W2114689657
Cited by
10
References
48
Citations by year
2025: 1 · 2024: 1 · 2020: 1 · 2019: 1 · 2018: 1 · 2017: 2 · 2015: 3
FWCI
1.47
Type
journal article
Language
en
Open access
Locations
  • FEBS Letters (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Irish Research Council for Science, Engineering and Technology (RS/2009/1245, RS/2012/407)
  • Science Foundation Ireland (09/IN.1/B2624)
Topics
  • Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ubiquitin and proteasome pathways
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › NF-κB Signaling Pathways
Keywords
presenilin 1, CUE domain, ubiquitin-binding domain, gamma-secretase, regulated intramembrane proteolysis, presenilin 2, amyloid precursor protein, notches, protein-protein interaction
MeSH terms
Amino Acid Sequence, Animals, Binding Sites, Cells, Cultured, Humans, Lysine, Molecular Sequence Data, Mutation, Peptide Fragments, Protein Binding, Blotting, Western, Signal Transduction, Amyloid beta-Peptides, Amyloid beta-Protein Precursor, Sequence Homology, Amino Acid, Ubiquitin, Polyubiquitin, Mice, Presenilin-1, Amyloid Precursor Protein Secretases, HEK293 Cells, Proteolysis

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review