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Cited by 170Sperm and Testicular FunctionReproductive Health and TechnologiesMale Reproductive Health Studies

Correlation between DNA damage and sperm parameters: a prospective study of 1,633 patients.

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Cohen-Bacrie, Paul, Belloc, Stephanie, Ménézo, Yves J.R., Clement, Patrice, Hamidi, Jamal, Benkhalifa, Moncef·2009·Fertility and Sterility
DOI 10.1016/j.fertnstert.2008.01.086
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Abstract

Objective To investigate DNA fragmentation by using terminal deoxyribonucleotidyl transferase-mediated dUTP nick-end labeling in relation to World Health Organization parameters and computer-aided sperm analysis (CASA) in sperm to determine the possibility of obtaining a correlation among CASA parameters, sperm morphology, and DNA fragmentation. Design Sperm analysis according to World Health Organization parameters, terminal deoxyribonucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) for sperm DNA fragmentation, and CASA for sperm movement. Prospective study. Setting All the patients were under clinical management, consulting for hypofertility at a fertility center in France. Patient(s) One thousand six hundred thirty-three men who were referred for infertility investigation, including a complete sperm analysis. Intervention(s) Sperm analysis and DNA damage testing. Main outcome measure(s) Sperm morphology, DNA fragmentation, and movement characteristics. Result(s) One third of the patients had a TUNEL rate of >30%. Analysis of the 21 semen parameters tested revealed that 7 of them were significantly correlated with the TUNEL results. Conclusion(s) World Health Organization sperm parameters and DNA damage are complementary, rather than strongly linked. This should be considered to more fully understand the paternal contribution in assisted reproductive technologies failures.

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Authors
Cohen-Bacrie, P., Belloc, S., Ménézo, Y. J. R., Clement, P., Hamidi, J., & Benkhalifa, M.
Corresponding author
Cohen-Bacrie, Paul
ORCID
Benkhalifa, Moncef
Venue
Fertility and Sterility
Publisher
Elsevier BV
Published
2009-05
Volume
91
Issue
5
Pages
1801-1805
ISSN
0015-0282 (ISSN-L)
DOI
10.1016/j.fertnstert.2008.01.086
OpenAlex
W2171354278
Cited by
170
References
1
Citations by year
2026: 3 · 2025: 4 · 2024: 10 · 2023: 4 · 2022: 9 · 2021: 13 · 2020: 5 · 2019: 15 · 2018: 10 · 2017: 6 · 2016: 8 · 2015: 13 · 2014: 13 · 2013: 18 · 2012: 6
FWCI
12.20
Type
journal article
Language
en
Open access
Licence
Locations
  • Fertility and Sterility (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Reproductive Medicine › Sperm and Testicular Function
  • Health Sciences › Medicine › Reproductive Medicine › Reproductive Health and Technologies
  • Health Sciences › Health Professions › General Health Professions › Male Reproductive Health Studies
Keywords
sperm DNA fragmentation, sperm DNA damage, TUNEL, computer-assisted sperm analysis, sperm morphology, sperm motility, male infertility, assisted reproductive technology failure, paternal contribution
MeSH terms
Adult, DNA Damage, Humans, Male, Prospective Studies, Sperm Motility, Spermatozoa, In Situ Nick-End Labeling, Sperm Injections, Intracytoplasmic, DNA Fragmentation

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