The in vitro antibacterial/synergistic activities of Withania somnifera extracts
Abstract
The methanol, hexane and diethyl ether extracts from both leaves and roots of Withania somnifera were evaluated for the antibacterial/synergistic activity by agar plate disc-diffusion assay against Salmonella typhimurium and Escherichia coli. Different concentrations of Tibrim, a combination of rifampicin and isoniazid, were tested to find out the minimum inhibitory concentration (MIC), which came out to be 0.1 mg/ml for S. typhimurium and E. coli. From the six extracts tested, only methanol and hexane extracts of both leaves and roots were found to have potent antibacterial activity. A synergistic increase in the antibacterial effect of Tibrim was noticed when MIC of Tibrim was supplemented with these extracts.
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Withania somnifera leaves and root (methanolic and hexane) extracts increase synergistic antibacterial activity of Tibrim against E. coli and Salmonella typhi.
“WS leaves and root (methanolic and hexane) extracts were also reported to increase the synergistic antibacterial activity of Tibrim (isoniazid and rifampicin) against E. coli and Salmonella typhi [29].”
From Withania somnifera (L.) Dunal (Ashwagandha): A comprehensive review on ethnopharmacology, pharmacotherapeutics, biomedicinal and toxicological aspects (2021) · cites this paper as [29]
Cites
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Paul, S., Chakraborty, S., Anand, U., Dey, S., Nandy, S., Ghorai, M., Saha, S. C., Patil, M. T., Kandimalla, R., Proćków, J., et al. (2021) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2021.112175 · Cited by 306 · see citing works
- Authors
- Arora, S., Dhillon, S., Rani, G., & Nagpal, A.
- Affiliations
- Guru Nanak Dev University (IN)
- Corresponding author
- Arora, S.
- ORCID
- Venue
- Fitoterapia
- Publisher
- Elsevier BV
- Published
- 2004-06
- Volume
- 75
- Issue
- 3-4
- Pages
- 385-388
- ISSN
- 0367-326X (ISSN-L)
- OpenAlex
- W1973948267
- Cited by
- 74
- References
- 8
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 1 · 2023: 7 · 2022: 5 · 2021: 4 · 2020: 6 · 2019: 4 · 2017: 4 · 2016: 5 · 2015: 6 · 2014: 7 · 2013: 2 · 2012: 5
- FWCI
- 2.46
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Fitoterapia (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Complementary and alternative medicine › Phytochemicals and Medicinal Plants
- Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Medicinal Plant Research
- Keywords
- Withania somnifera, antibacterial activity, synergistic activity, plant extracts, methanol extract, hexane extract, diethyl ether extract, leaf extract, root extract, Salmonella typhimurium, Escherichia coli, rifampicin, isoniazid, minimum inhibitory concentration
- MeSH terms
- Anti-Bacterial Agents, Drug Synergism, Escherichia coli, Humans, Isoniazid, Phytotherapy, Microbial Sensitivity Tests, Plant Extracts, Rifampin, Salmonella typhimurium, Plant Leaves, Plant Roots, Withania
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