Description of the bacteriocins produced by two strains of Enterococcus faecium isolated from Italian goat milk

COCOLIN, L; FOSCHINO, R; COMI, G; GRAZIAFORTINA, M

doi:10.1016/j.fm.2007.03.001

Cited by 106Probiotics and Fermented FoodsListeria monocytogenes in Food SafetyBacterial Genetics and Biotechnology

Description of the bacteriocins produced by two strains of Enterococcus faecium isolated from Italian goat milk

COCOLIN, L, FOSCHINO, R, COMI, G, GRAZIAFORTINA, M·2007·Food Microbiology

DOI 10.1016/j.fm.2007.03.001

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Abstract

In this study two strains of Enterococcus faecium, M241 and M249, isolated from goat milk, were studied for their capability to produce antibacterial compounds. It was determined that the bacteriocins produced by both strains were active towards Listeria monocytogenes and Clostridium butyricum, and they did not have any activity with respect to other species of lactic acid bacteria. Enterocins A and B were targeted by polymerase chain reaction (PCR) and sequenced, after cloning, in both strains. The bacteriocins contained in the cell free supernatants were stable when subjected to treatments at high and low temperatures or with lipase, catalase and alpha-amylase. Whereas, the activity was lost when proteinases were used. Lastly, a co-culture experiment with L. monocytogenes in skimmed milk was performed. In the presence of the E. faecium strains, the pathogen showed a delay in the growth of about 6h and it reached a maximum counts of about 10(6) colony forming unit, two orders of magnitude low with respect to the control. This result suggests the possibility to use the strains studied as starter cultures to enhance food safety of dairy products.

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Some strains of enterococci have been identified as potential pathogens, so the presence of virulence factors and resistance to certain antibiotics should be carefully evaluated before using them.
“Some strains of enterococci have been identified as potential pathogens, so the presence of virulence factors and resistance to certain antibiotics should be carefully evaluated before using them [82,83,84,85].”
From Lactic Acid Bacteria in Raw-Milk Cheeses: From Starter Cultures to Probiotic Functions (2022) · cites this paper as [85]

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Authors: COCOLIN, L., FOSCHINO, R., COMI, G., & GRAZIAFORTINA, M.
Affiliations: University of Turin (IT)
University of Milan (IT)
University of Udine (IT)
Corresponding author: COCOLIN, L
ORCID: COCOLIN, L
FOSCHINO, R
COMI, G
Venue: Food Microbiology
Publisher: Elsevier BV
Published: 2007-10
Volume: 24
Issue: 7-8
Pages: 752-758
ISSN: 0740-0020 (ISSN-L)
DOI: 10.1016/j.fm.2007.03.001
OpenAlex: W2035858149
Cited by: 106
References: 43
Citations by year: 2026: 1 · 2024: 3 · 2023: 6 · 2022: 9 · 2021: 4 · 2020: 6 · 2019: 3 · 2018: 8 · 2017: 3 · 2016: 11 · 2015: 8 · 2014: 7 · 2013: 5 · 2012: 13
FWCI: 6.50
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Food Microbiology (publishedVersion)
PubMed (publishedVersion)
Institutional Research Information System (University of Udine) (submittedVersion)
Topics: Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Listeria monocytogenes in Food Safety
Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords: Enterococcus faecium, bacteriocins, goat milk, Listeria monocytogenes, Clostridium butyricum, lactic acid bacteria, antibacterial activity, bacteriocin stability, protease sensitivity, co-culture, starter cultures, dairy product safety
MeSH terms: Animals, Anti-Bacterial Agents, Bacteriocins, Consumer Product Safety, Food Microbiology, Goats, Humans, Italy, Listeria monocytogenes, Milk, Colony Count, Microbial, Enterococcus faecium, Coculture Techniques, Clostridium butyricum

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Description of the bacteriocins produced by two strains of Enterococcus faecium isolated from Italian goat milk

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