Purification, antitumor and antioxidant activities in vitro of polysaccharides from the brown seaweed Sargassum pallidum
Abstract
Supercritical CO2 extraction, ultrasonic-aid extraction and membrane separation technology were applied to prepare Sargassum pallidum polysaccharides (SP). Three main fractions, SP-1, SP-2 and SP-3, were obtained by membranes of 1.0×10(-4)mm pore size and normal molecular-weight cut-off of 50kDa. The resulting three preparations were further purified by DEAE Cellulose-52 chromatography to afford seven polysaccharide fractions. Furthermore, the antitumor and antioxidant activities, in vitro, of the polysaccharide fractions were evaluated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay and DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical-scavenging assay, respectively. SP-3-1 and SP-3-2 showed significantly higher antitumor activity against the HepG2 cells, A549 cells, and MGC-803 cells. SP-3 had the highest sulfate content (22.6%). These results indicate that the higher antitumor activity of SP-3-1 and SP-3-2 from SP-3 with lower molecular weights may be related to their molecular weights and sulfate contents. The antioxidant activities of SP-1, SP-2 and SP-3 were low at the tested concentration.
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Algae are a good source of dietary fibers because of non-digestible polysaccharides in the algal cell wall, which possess antioxidant, hypoglycemic, antiviral, antitumor, and anti-inflammatory activities due to fucoidan and laminarin.
“Algae are considered as a good source of dietary fibers because of the presence of non-digestible polysaccharides in the algal cell wall (Figure 3) [11]. It has been shown that they possess activities, like antioxidant [12], hypoglycemic [13], antiviral [14,15], antitumor [16], and anti-inflammatory due to the presence of fucoidan and laminarin [17].”
From Overview on the Application of Modern Methods for the Extraction of Bioactive Compounds from Marine Macroalgae (2018) · cites this paper as [16]
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References 26 · Cited by 372
- Authors
- Ye, H., Wang, K., Zhou, C., Liu, J., & Zeng, X.
- Affiliations
- Nanjing Agricultural University (CN)
- Corresponding author
- Zeng, Xiaoxiong
- Venue
- Food Chemistry
- Publisher
- Elsevier BV
- Published
- 2008-11
- Volume
- 111
- Issue
- 2
- Pages
- 428-432
- ISSN
- 0308-8146 (ISSN-L)
- OpenAlex
- W1989625938
- Cited by
- 372
- References
- 26
- Citations by year
- 2026: 6 · 2025: 8 · 2024: 12 · 2023: 12 · 2022: 19 · 2021: 26 · 2020: 23 · 2019: 27 · 2018: 23 · 2017: 25 · 2016: 30 · 2015: 26 · 2014: 26 · 2013: 34 · 2012: 30
- FWCI
- 22.53
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Food Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China
- Nanjing University
- Nanjing Agricultural University
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Physical Sciences › Earth and Planetary Sciences › Oceanography › Marine and coastal plant biology
- Keywords
- Sargassum pallidum, brown seaweed polysaccharides, polysaccharide purification, anticancer activity, antioxidant activity, supercritical CO2 extraction, ultrasound-assisted extraction, membrane separation, DEAE-cellulose chromatography, molecular weight, sulfate content, HepG2 cells, A549 cells, MGC-803 cells, free radical scavenging
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