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Cited by 57Autophagy in Disease and TherapyCannabis and Cannabinoid ResearchNatural Compounds in Disease Treatment

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Cannabisin B induces autophagic cell death by inhibiting the AKT/mTOR pathway and S phase cell cycle arrest in HepG2 cells

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Chen, Tianpeng, Hao, Jianxiong, He, Jinfeng, Zhang, Jianchun, Li, Yingcong, Liu, Rui, Li, Lite·2013·Food Chemistry
DOI 10.1016/j.foodchem.2012.11.102
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Abstract

This study investigates the anticancer properties of cannabisin B, purified from hempseed hull, in HepG2 human hepatoblastoma cells. The results indicate that cannabisin B significantly inhibited cell proliferation by inducing autophagic cell death rather than typical apoptosis. Cell viability transiently increased upon the addition of a low concentration of cannabisin B but decreased upon the addition of high concentrations. Cannabisin B-induced changes in cell viability were completely inhibited by pre-treatment with 3-methyladenine (3-MA), indicating that the induction of autophagy by cannabisin B caused cell death. Additionally, cannabisin B induced S phase cell cycle arrest in a dose-dependent manner. Moreover, cannabisin B was found to inhibit survival signaling by blocking the activation of AKT and down-stream targets of the mammalian target of rapamycin (mTOR). These findings suggest that cannabisin B possesses considerable antiproliferative activity and that it may be utilised as a promising chemopreventive agent against hepatoblastoma disease.

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References 28 · Cited by 57

Research works printed but not linked: 24

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Metadata

Authors
Chen, T., Hao, J., He, J., Zhang, J., Li, Y., Liu, R., & Li, L.
Affiliations
  • Peking University (CN)
  • Hebei University of Science and Technology (CN)
  • Chinese Academy of Sciences (CN)
  • China Agricultural University (CN)
ORCID
Venue
Food Chemistry
Publisher
Elsevier BV
Published
2013-06
Volume
138
Issue
2-3
Pages
1034-1041
ISSN
0308-8146 (ISSN-L)
DOI
10.1016/j.foodchem.2012.11.102
OpenAlex
W2040307089
Cited by
57
References
28
Citations by year
2026: 2 · 2025: 3 · 2024: 6 · 2023: 7 · 2022: 7 · 2021: 5 · 2020: 10 · 2019: 3 · 2018: 5 · 2017: 2 · 2016: 3 · 2015: 2 · 2014: 1 · 2013: 1
FWCI
0.95
Type
journal article
Language
en
Open access
Licence
Locations
  • Food Chemistry (publishedVersion)
  • PubMed (publishedVersion)
  • Institutional Repository of Institute of Psychology, Chinese Academy of Sciences (Institute of Psychology, Chinese Academy of Sciences) (submittedVersion, cc-by-nc-sa)
  • Institutional Repository of Institute of Psychology, Chinese Academy of Sciences (Institute of Psychology, Chinese Academy of Sciences) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Autophagy in Disease and Therapy
  • Health Sciences › Medicine › Pharmacology (medical) › Cannabis and Cannabinoid Research
  • Health Sciences › Medicine › Complementary and alternative medicine › Natural Compounds in Disease Treatment
Keywords
autophagic cell death, Akt/mTOR signaling, S phase arrest, hepatoblastoma, HepG2 cells, antiproliferative activity, chemoprevention, 3-methyladenine, plant-derived anticancer agents
MeSH terms
Autophagy, Cannabis, Humans, Plant Extracts, Seeds, Signal Transduction, Down-Regulation, Proto-Oncogene Proteins c-akt, Hep G2 Cells, TOR Serine-Threonine Kinases, S Phase Cell Cycle Checkpoints

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CoChat. (2026). Citation check: Cannabisin B induces autophagic cell death by inhibiting the AKT/mTOR pathway and S phase cell cycle arrest in HepG2 cells. https://app.cochat.ai/papers/10.1016/j.foodchem.2012.11.102
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Chen, T., Hao, J., He, J., Zhang, J., Li, Y., Liu, R., & Li, L. (2013). Cannabisin B induces autophagic cell death by inhibiting the AKT/mTOR pathway and S phase cell cycle arrest in HepG2 cells. Food Chemistry. https://doi.org/10.1016/j.foodchem.2012.11.102

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