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Effects of enzymatic hydrolysis on conformational and functional properties of chickpea protein isolate

Mokni Ghribi, Abir, Maklouf Gafsi, Ines, Sila, Assaâd, Blecker, Christophe, Danthine, Sabine, Attia, Hamadi, Bougatef, Ali, Besbes, Souhail·2015·Food Chemistry

Abstract

The impact of enzymatic hydrolysis by Alcalase on the conformational and functional properties of chickpea protein isolate (CPI) was investigated. The physicochemical, interfacial tension and surface characteristics of CPI and their hydrolysates (CPH) according to the degree of hydrolysis (DH) were also determined. These parameters were then related to the changes in the emulsification activity (EAI) and stability (ESI). The enzymatic hydrolysis was found to improve protein recovery and solubility, leading to a reduction in the molecular weight bands with a concomitant increase in the intensity and appearance of protein bands having apparent molecular mass below 20 kDa. The interfacial tension decreased from ∼ 66.5 mN m(-1) for CPI to ∼ 59.1 m Nm(-1) for CPH. A similar trend was observed for the surface charge which declined from -27.55 mV to -16.4 mV for the CPI and CPH, respectively. These changes were found to have a detrimental effect on the EAI and ESI values.

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Metadata

Authors
Mokni Ghribi, A., Maklouf Gafsi, I., Sila, A., Blecker, C., Danthine, S., Attia, H., Bougatef, A., & Besbes, S.
Venue
Food Chemistry
Publisher
Elsevier BV
Published
2015-11
Volume
187
Pages
322-330
ISSN
0308-8146 (ISSN-L)
OpenAlex
W2040444872
Cited by
355
References
0
FWCI
15.05
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: Effects of enzymatic hydrolysis on conformational and functional properties of chickpea protein isolate. https://app.cochat.ai/papers/10.1016/j.foodchem.2015.04.109
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Mokni Ghribi, A., Maklouf Gafsi, I., Sila, A., Blecker, C., Danthine, S., Attia, H., Bougatef, A., & Besbes, S. (2015). Effects of enzymatic hydrolysis on conformational and functional properties of chickpea protein isolate. Food Chemistry. https://doi.org/10.1016/j.foodchem.2015.04.109

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