Biorefining of industrial hemp (Cannabis sativa L.) threshing residues into cannabinoid and antioxidant fractions by supercritical carbon dioxide, pressurized liquid and enzyme-assisted extractions
Abstract
C. sativa threshing residues were biorefined by consecutive supercritical carbon dioxide (SFE-CO 2) pressurised liquid (PLE) and enzyme-assisted extractions (EAE). SFE-CO 2 at optimised parameters yielded 8.3g/100g of lipophilic fraction containing 0.2 and 2.2g of cannabidiol and cannabidiolic acid per 100g of threshing residues, respectively. The recovery of cannabinoids from plant material was >93%. PLE gave 4.3 and 18.9g/100g of flavonoid-containing polar extracts, while EAE added 20.2% (w/w) of water-soluble constituents and increased the release of mono- and disaccharides by up to 94%. Antioxidant capacity of non-polar and polar fractions was in the range of 1.3-23.5mg gallic acid equivalents/g DW and 0.6-205.2mg Trolox equivalents/g DW, with the highest activities of PLE-EtOH/H 2 O extract. The combined SFE-CO 2, PLE and EAE reduced antioxidant capacity of starting plant material by 90-99%, showing that suggested multistep fractionation procedure is efficient in the recovery of a major part of the antioxidatively active constituents from hemp threshing residues.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The whole sequential process reduced the raw material by 90-99%.
“The whole sequential process reduced the raw material by 90–99%.”
From Supercritical Carbon Dioxide Technology for Recovering Valuable Phytochemicals from Cannabis sativa L. and Valorization of Its Biomass for Food Applications (2023) · cites this paper as [92]
Supercritical CO2 extraction recovered more than 93% of the cannabinoids from threshing residue.
“Supercritical CO2 extraction was used to obtain the lipophilic fraction containing fatty acids and cannabinoids, recovering more than 93% of the cannabinoids from the raw material.”
From Supercritical Carbon Dioxide Technology for Recovering Valuable Phytochemicals from Cannabis sativa L. and Valorization of Its Biomass for Food Applications (2023) · cites this paper as [92]
Cites
Cannabis sativa: The Plant of the Thousand and One Molecules
Andre, C. M., Hausman, J.-F., & Guerriero, G. (2016) · Frontiers in Plant Science · doi:10.3389/fpls.2016.00019 · Cited by 1,544 · see citing works
Secondary metabolism in cannabis
Flores-Sanchez, I. J., & Verpoorte, R. (2008) · Phytochemistry Reviews · doi:10.1007/s11101-008-9094-4 · Cited by 400 · see citing works
Da Porto, C., Decorti, D., & Tubaro, F. (2012) · Industrial Crops and Products · doi:10.1016/j.indcrop.2011.09.015 · Cited by 236 · see citing works
Characterization of Byproducts Originating from Hemp Oil Processing
Pojić, M., Mišan, A., Sakač, M., Dapčević Hadnađev, T., Šarić, B., Milovanović, I., & Hadnađev, M. (2014) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf5044426 · Cited by 181 · see citing works
Chen, T., He, J., Zhang, J., Li, X., Zhang, H., Hao, J., & Li, L. (2012) · Food Chemistry · doi:10.1016/j.foodchem.2012.03.009 · Cited by 167 · see citing works
Da Porto, C., Decorti, D., & Natolino, A. (2014) · Industrial Crops and Products · doi:10.1016/j.indcrop.2014.03.042 · Cited by 99 · see citing works
Da Porto, C., Natolino, A., & Decorti, D. (2015) · Journal of Food Science and Technology · doi:10.1007/s13197-013-1143-3 · Cited by 75 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Baldino, L., Scognamiglio, M., & Reverchon, E. (2020) · The Journal of Supercritical Fluids · doi:10.1016/j.supflu.2020.104960 · Cited by 129 · see citing works
Isidore, E., Karim, H., & Ioannou, I. (2021) · Antioxidants · doi:10.3390/antiox10060942 · Cited by 110 · see citing works
Moreno, T., Montanes, F., Tallon, S. J., Fenton, T., & King, J. W. (2020) · The Journal of Supercritical Fluids · doi:10.1016/j.supflu.2020.104850 · Cited by 100 · see citing works
de Aguiar, A. C., Vardanega, R., Viganó, J., & Silva, E. K. (2023) · Molecules · doi:10.3390/molecules28093849 · Cited by 51 · see citing works
“[92] proposed biorefining the threshing into cannabinoids and antioxidant fractions.”
BackgroundSupercritical fluids and fluid mixtures to obtain high-value compounds from Capsicum peppers
De Aguiar, A. C., Viganó, J., da Silva Anthero, A. G., Dias, A. L. B., Hubinger, M. D., & Martínez, J. (2022) · Food Chemistry: X · doi:10.1016/j.fochx.2022.100228 · Cited by 45 · see citing works
Drinić, Z., Vladic, J., Koren, A., Zeremski, T., Stojanov, N., Tomić, M., & Vidović, S. (2021) · Industrial Crops and Products · doi:10.1016/j.indcrop.2021.113908 · Cited by 46 · see citing works
Kornpointner, C., Sainz Martinez, A., Schnürch, M., Halbwirth, H., & Bica-Schröder, K. (2021) · Green Chemistry · doi:10.1039/d1gc03516a · Cited by 28 · see citing works
Cannabinoids Enriched Extracts from Industrial Hemp Residues
Vági, E., Balázs, M., Komóczi, A., Kiss, I., Mihalovits, M., & Székely, E. (2019) · Periodica Polytechnica Chemical Engineering · doi:10.3311/ppch.12896 · Cited by 27 · see citing works
Cannabis Extraction Technologies: Impact of Research and Value Addition in Latin America
Suárez-Jacobo, Á., Díaz Pacheco, A., Bonales-Alatorre, E., Castillo-Herrera, G. A., & García-Fajardo, J. A. (2023) · Molecules · doi:10.3390/molecules28072895 · Cited by 13 · see citing works
- Authors
- Kitrytė, V., Bagdonaitė, D., & Rimantas Venskutonis, P.
- Affiliations
- Kaunas University of Technology (LT)
- ORCID
- Kitrytė, Vaida
- Venue
- Food Chemistry
- Publisher
- Elsevier BV
- Published
- 2018-11
- Volume
- 267
- Pages
- 420-429
- ISSN
- 0308-8146 (ISSN-L)
- OpenAlex
- W2754568794
- Cited by
- 103
- References
- 7
- Citations by year
- 2026: 2 · 2025: 9 · 2024: 8 · 2023: 17 · 2022: 21 · 2021: 18 · 2020: 13 · 2019: 13 · 2018: 2
- FWCI
- 5.36
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Food Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Funders
- JSC Agropro (8743)
- Topics
- Health Sciences › Medicine › Pharmacology › Cannabis and Cannabinoid Research
- Physical Sciences › Engineering › Biomedical Engineering › Phase Equilibria and Thermodynamics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Agricultural and Biological Research
- Keywords
- industrial hemp, Cannabis sativa, biorefining, supercritical CO2 extraction, accelerated solvent extraction, enzymatic extraction, cannabinoids, cannabidiol, cannabidiolic acid, flavonoids, antioxidant capacity, polysaccharide recovery
- MeSH terms
- Ethanol, Antioxidants, Biotechnology, Cannabidiol, Cannabinoids, Cannabis, Carbon Dioxide, Chemical Fractionation, Plant Extracts, Solubility, Water
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
