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Taste and chirality: l-glucose sweetness is mediated by TAS1R2/TAS2R3 receptor
Open accessPublished version of a preprint
Abstract
Naturally occurring sugars usually have d-chirality. While a change in chirality typically affects ligand-receptor interaction, non-caloric l-glucose was reported as sweet for humans. Here we show that l- and d-glucose have similar sensory detection thresholds (0.041 ± 0.006 M for d-glucose, and 0.032 ± 0.007 M for l-glucose) and similar sweetness intensities at suprathreshold concentrations. We demonstrate that l-glucose acts via the sweet taste receptor TAS1R2/TAS1R3, eliciting a dose-dependent activation in cell-based functional assays. Computational docking of glucose to the VFT domain of TAS1R2 suggests two sub-pockets, each compatible with each of the enantiomers. While some polar residues (Y103, D142, N143, S144, Y215) are unique for sub-pocket A and others (D307, T326, E382, R383) for sub-pocket B, no interaction is unique for only one enantiomer. The many options for creating hydrogen bonds with the hydroxyl moieties of glucose explain how both enantiomers can fit each one of the sub-pockets.
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Metadata
- Authors
- Dubovski, N., Ben Shoshan-Galeczki, Y., Malach, E., & Niv, M. Y.
- Affiliations
- Hebrew University of Jerusalem (IL)
- Corresponding author
- Niv, Masha Y.
- Venue
- Food Chemistry
- Publisher
- Elsevier BV
- Published
- 2022-03
- Volume
- 373
- Issue
- Pt A
- Pages
- 131393
- ISSN
- 0308-8146 (ISSN-L)
- OpenAlex
- W3207151512
- Cited by
- 28
- References
- 4
- Citations by year
- 2026: 6 · 2025: 9 · 2024: 5 · 2023: 5 · 2022: 3
- FWCI
- 2.08
- Type
- journal article
- Language
- en
- Open access
- green
- doi.org
- Licence
- Locations
- Food Chemistry (publishedVersion)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Funders
- Israel Science Foundation (#1129/19)
- Topics
- Health Sciences › Nursing › Nutrition and Dietetics › Biochemical Analysis and Sensing Techniques
- Life Sciences › Neuroscience › Sensory Systems › Ion Channels and Receptors
- Life Sciences › Agricultural and Biological Sciences › Food Science › Sensory Analysis and Statistical Methods
- Keywords
- L-glucose, D-glucose, sweet taste receptor, ligand-receptor interactions, molecular docking, hydrogen bonding, sensory detection threshold, artificial sweeteners
- MeSH terms
- Sugars, Glucose, Humans, Hydrogen Bonding, Taste, Receptors, G-Protein-Coupled
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