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Cited by 59Protein Hydrolysis and Bioactive PeptidesInfant Nutrition and HealthAnimal Diversity and Health Studies

Comprehensive peptidomic and glycomic evaluation reveals that sweet whey permeate from colostrum is a source of milk protein-derived peptides and oligosaccharides

Open access
Dallas, David C., Weinborn, Valerie, de Moura Bell, Juliana M.L.N., Wang, Meng, Parker, Evan A., Guerrero, Andres, Hettinga, Kasper A., Lebrilla, Carlito B. +2 more·2014·Food Research International
DOI 10.1016/j.foodres.2014.03.021PMC PMC4180214
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Abstract

Whey permeate is a co-product obtained when cheese whey is passed through an ultrafiltration membrane to concentrate whey proteins. Whey proteins are retained by the membrane, whereas the low-molecular weight compounds such as lactose, salts, oligosaccharides and peptides pass through the membrane yielding whey permeate. Research shows that bovine milk from healthy cows contains hundreds of naturally occurring peptides - many of which are homologous with known antimicrobial and immunomodulatory peptides - and nearly 50 oligosaccharide compositions (not including structural isomers). As these endogenous peptides and oligosaccharides have low-molecular weight and whey permeate is currently an under-utilized product stream of the dairy industry, we hypothesized that whey permeate may serve as an inexpensive source of naturally occurring functional peptides and oligosaccharides. Laboratory fractionation of endogenous peptides and oligosaccharides from bovine colostrum sweet whey was expanded to pilot-scale. The membrane fractionation methodology used was similar to the methods commonly used industrially to produce whey protein concentrate and whey permeate. Pilot-scale fractionation was compared to laboratory-scale fractionation with regard to the identified peptides and oligosaccharide compositions. Results were interpreted on the basis of whether industrial whey permeate could eventually serve as a source of functional peptides and oligosaccharides. The majority (96%) of peptide sequences and the majority (96%) of oligosaccharide compositions found in the laboratory-scale process were mirrored in the pilot-scale process. Moreover, the pilot-scale process recovered an additional 33 peptides and 1 oligosaccharide not identified from the laboratory-scale extraction. Both laboratory- and pilot-scale processes yielded peptides deriving primarily from the protein β-casein. The similarity of the laboratory-and pilot-scale's resulting peptide and oligosaccharide profiles demonstrates that whey permeate can serve as an industrial-scale source of bovine milk peptides and oligosaccharides.

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  • Bovine colostrum contains a limited amount of milk oligosaccharides that may promote the growth of B. infantis.

    “While bovine colostrum not only contains a limited amount of milk oligosaccharides that may promote the growth of this particular bacteria [35, 36], it also contains an abundance of immune proteins, including immunoglobulins, lactoferrin and a range of cytokines, that can further modulate the microbiota [40] and the immune system [40–42].”

    From Pilot study of probiotic/colostrum supplementation on gut function in children with autism and gastrointestinal symptoms (2019) · cites this paper as [35]

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  1. Paquet D Alais C Aubert F Review: The proteose-peptone fraction of milk Le Lait 1989 69 1 1 21

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Authors
Dallas, D. C., Weinborn, V., de Moura Bell, J. M. L. N., Wang, M., Parker, E. A., Guerrero, A., Hettinga, K. A., Lebrilla, C. B., German, J. B., & Barile, D.
Affiliations
  • University of California, Davis (US)
  • Wageningen University & Research (NL)
Corresponding author
Dallas, David C.
ORCID
Venue
Food Research International
Publisher
Elsevier BV
Published
2014-09
Volume
63
Issue
Pt B
Pages
203-209
ISSN
0963-9969 (ISSN-L)
DOI
10.1016/j.foodres.2014.03.021
PMC
PMC4180214
OpenAlex
W1995076766
Cited by
59
References
45
Citations by year
2026: 4 · 2025: 2 · 2024: 5 · 2023: 3 · 2022: 4 · 2021: 7 · 2020: 10 · 2019: 7 · 2018: 6 · 2017: 2 · 2016: 2 · 2015: 5 · 2014: 2
FWCI
1.50
Type
journal article
Language
en
Open access
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Food Research International (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
  • Socio-Environmental Systems Modeling (acceptedVersion)
  • eScholarship (California Digital Library) (submittedVersion)
  • Data Archiving and Networked Services (DANS) (submittedVersion)
Funders
  • Bill and Melinda Gates Foundation
  • National Institutes of Health (R01 AT007079, R01 HD059127, UL1 TR000002)
  • Peter J. Shields Endowed Chair in Dairy Food Science
  • National Science Foundation
  • USDA
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Hydrolysis and Bioactive Peptides
  • Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Animal Diversity and Health Studies
Keywords
whey permeate, colostrum, oligosaccharides, cow's milk, β-casein, functional peptides, dairy by-products, antimicrobial peptides, immunomodulatory peptides, glycomics, peptidomics

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