Antioxidant supplementation prevents exercise-induced lipid peroxidation, but not inflammation, in ultramarathon runners
Abstract
To determine if 6 weeks of supplementation with vitamins E and C could alleviate exercise-induced lipid peroxidation and inflammation, we studied 22 runners during a 50 km ultramarathon. Subjects were randomly assigned to one of two groups: (1) placebos (PL) or (2) antioxidants (AO: 1000 mg vitamin C and 300 mg RRR-alpha-tocopheryl acetate). Blood samples were obtained prior to supplementation (baseline), after 3 weeks of supplementation, 1 h pre-, mid-, and postrace, 2 h postrace and for 6 days postrace. Plasma levels of alpha-tocopherol (alpha-TOH), ascorbic acid (AA), uric acid (UA), F2-isoprostanes (F2-IsoPs), tumor necrosis factor alpha (TNF-alpha), interleukin-6 (IL-6), and C-reactive protein (CRP) were measured. With supplementation, plasma alpha-TOH and AA increased in the AO but not the PL group. Although F2-IsoP levels were similar between groups at baseline, 28 +/- 2 (PL) and 27 +/- 3 pg/ml (AO), F2-IsoPs increased during the run only in the PL group (41 +/- 3 pg/ml). In PL women, F2-IsoPs were elevated postrace (p <.01), but returned to prerace concentrations by 2 h postrace. In PL men, F2-IsoP concentrations were higher postrace, 2 h postrace, and 1, 2, 3, 4, and 6 days postrace (PL vs. AO group, each p <.03). Markers of inflammation were increased dramatically in response to the run regardless of treatment group. Thus, AO supplementation prevented endurance exercise-induced lipid peroxidation but had no effect on inflammatory markers.
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Ageing is associated with increased concentrations of TNF-a, CRP, and IL-1ra.
“Ageing is also associated with increased concentrations of TNF-a (178,179) , CRP (180) and IL-1ra (176,181) .”
From Dietary factors and low-grade inflammation in relation to overweight and obesity (2011) · cites this paper as [178]
In a 31-mile race, a significant protective effect against lipid peroxidation was observed in runners supplemented with α-tocopherol 300 mg·d−1 and ascorbic acid 1000 mg·d−1 for 7 weeks prior.
“Moreover, in a 31-mile (50 km) race, Mastaloudis, et al. [173] observed a significant protective effect against lipid peroxidation in runners who had been supplemented with antioxidants (α-tocopherol at 300 mg·d− 1, and ascorbic acid 1000 mg·d− 1) for 7 weeks prior.”
From International Society of Sports Nutrition Position Stand: nutritional considerations for single-stage ultra-marathon training and racing (2019) · cites this paper as [173]
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References 82 · Cited by 244
- Authors
- Mastaloudis, A., Morrow, J. D., Hopkins, D. W., Devaraj, S., & Traber, M. G.
- Affiliations
- Oregon State University (US)
- Vanderbilt University (US)
- University of California Davis Medical Center (US)
- Corresponding author
- Mastaloudis, Angela
- Venue
- Free Radical Biology and Medicine
- Publisher
- Elsevier BV
- Published
- 2004-05
- Volume
- 36
- Issue
- 10
- Pages
- 1329-1341
- ISSN
- 0891-5849 (ISSN-L)
- OpenAlex
- W2079975516
- Cited by
- 244
- References
- 82
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 3 · 2023: 5 · 2022: 5 · 2021: 7 · 2020: 7 · 2019: 5 · 2018: 9 · 2017: 7 · 2016: 15 · 2015: 7 · 2014: 11 · 2013: 13 · 2012: 16
- FWCI
- 14.57
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Free Radical Biology and Medicine (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Cancer Institute (CA77839, P01CA077839)
- National Institute of General Medical Sciences (GM 15431)
- National Institute of Diabetes and Digestive and Kidney Diseases (DK 48831, R01DK048831)
- National Institute of Environmental Health Sciences (R03ES011536)
- National Center for Research Resources (RR00095)
- National Center for Complementary and Integrative Health (K-24AT00596, K24AT000596)
- Topics
- Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Health Sciences › Medicine › Complementary and alternative medicine › Cardiovascular and exercise physiology
- Keywords
- antioxidant supplementation, vitamin E, vitamin C, lipid peroxidation, exercise-induced inflammation, ultramarathon, endurance exercise, F2-isoprostanes, alpha-tocopherol, TNF-α, interleukin-6, C-reactive protein, uric acid
- MeSH terms
- Adolescent, Adult, Antioxidants, Ascorbic Acid, C-Reactive Protein, Female, Humans, Inflammation, Male, Middle Aged, Running, Tumor Necrosis Factor-alpha, Uric Acid, Vitamin E, Lipid Peroxidation, Exercise, Interleukin-6, Dietary Supplements, alpha-Tocopherol, F2-Isoprostanes
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