The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.
Nox5 mediates PDGF-induced proliferation in human aortic smooth muscle cells
Open accessAbstract
The proliferation of vascular smooth muscle cells is important in the pathogenesis of many vascular diseases. Reactive oxygen species (ROS) produced by NADPH oxidases in smooth muscle cells have been shown to participate in signaling cascades regulating proliferation induced by platelet-derived growth factor (PDGF), a powerful smooth muscle mitogen. We sought to determine the role of Nox5 in the regulation of PDGF-stimulated human aortic smooth muscle cell (HASMC) proliferation. Cultured HASMC were found to express four isoforms of Nox5. When HASMC stimulated with PDGF were pretreated with N-acetyl cysteine (NAC), proliferation was significantly reduced. Proliferation induced by PDGF was also heavily dependent on JAK/STAT activation, as the JAK inhibitor, AG490, was able to completely abolish PDGF-stimulated HASMC growth. Specific knockdown of Nox5 with a siRNA strategy reduced PDGF-induced HASMC ROS production and proliferation. Additionally, siRNA to Nox5 inhibited PDGF-stimulated JAK2 and STAT3 phosphorylation. ROS produced by Nox5 play an important role in PDGF-induced JAK/STAT activation and HASMC proliferation.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“As indicated above, the particular architecture of NOX5 indicates a regulation of its activity through Ca2+ levels (62, 469).”
From Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation (2019) · cites this paper as [469]
In the citing paper
“In human blood vessels, NOX5 is expressed in ECs and VSMCs (81, 469) (FIGURE 9, Table 5).”
From Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation (2019) · cites this paper as [469]
Citations
Cites
Requirement for Generation of H 2 O 2 for Platelet-Derived Growth Factor Signal Transduction
Sundaresan, M., Yu, Z.-X., Ferrans, V. J., Irani, K., & Finkel, T. (1995) · Science · doi:10.1126/science.270.5234.296 · Cited by 2,520
The JAK/STAT signaling pathway
Rawlings, J. S., Rosler, K. M., & Harrison, D. A. (2004) · Journal of Cell Science · doi:10.1242/jcs.00963 · Cited by 1,858
Ross, R., Glomset, J. A., Kariya, B., & Harker, L. A. (1974) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.71.4.1207 · Cited by 1,848
Inhibition of Neointimal Smooth Muscle Accumulation After Angioplasty by an Antibody to PDGF
Ferns, G. A., Raines, E. W., Sprugel, K. H., Motani, A. S., Reidy, M. A., & Ross, R. (1991) · Science · doi:10.1126/science.1653454 · Cited by 1,064
Lassègue, B., Sorescu, D., Szöcs, K., Yin, Q., Akers, M., Zhang, Y., Grant, S. L., Lambeth, J. D., & Griendling, K. K. (2001) · Circulation Research · doi:10.1161/hh0901.090299 · Cited by 839
Self-citationHomologs of gp91 phox : cloning and tissue expression of Nox3, Nox4, and Nox5
Cheng, G., Cao, Z., Xu, X., Meir, E. G. V., & Lambeth, J. D. (2001) · Gene · doi:10.1016/s0378-1119(01)00449-8 · Cited by 820
Cucoranu, I., Clempus, R., Dikalova, A., Phelan, P. J., Ariyan, S., Dikalov, S., & Sorescu, D. (2005) · Circulation Research · doi:10.1161/01.res.0000187457.24338.3d · Cited by 786
Self-citationA Ca2+-activated NADPH Oxidase in Testis, Spleen, and Lymph Nodes
Bánfi, B., Molnár, G., Maturana, A., Steger, K., Hegedûs, B., Demaurex, N., & Krause, K.-H. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m103034200 · Cited by 622
Activation of the JAK-STAT pathway by reactive oxygen species
Simon, A. R., Rai, U., Fanburg, B. L., & Cochran, B. (1998) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.1998.275.6.c1640 · Cited by 597
Distinct Subcellular Localizations of Nox1 and Nox4 in Vascular Smooth Muscle Cells
Hilenski, L. L., Clempus, R. E., Quinn, M. T., Lambeth, J. D., & Griendling, K. K. (2004) · Arteriosclerosis, Thrombosis, and Vascular Biology · doi:10.1161/01.atv.0000112024.13727.2c · Cited by 573
Self-citationMeasurement of Reactive Oxygen Species in Cardiovascular Studies
Dikalov, S., Griendling, K. K., & Harrison, D. G. (2007) · Hypertension · doi:10.1161/01.hyp.0000258594.87211.6b · Cited by 532
Self-citationPDGF and cardiovascular disease
Raines, E. W. (2004) · Cytokine & Growth Factor Reviews · doi:10.1016/j.cytogfr.2004.03.004 · Cited by 496
Mechanism of Ca2+ Activation of the NADPH Oxidase 5 (NOX5)
Bánfi, B., Tirone, F., Durussel, I., Knisz, J., Moskwa, P., Molnár, G. Z., Krause, K.-H., & Cox, J. A. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m310268200 · Cited by 389
Reactive oxygen species signaling in vascular smooth muscle cells
Clempus, R. E., & Griendling, K. K. (2006) · Cardiovascular Research · doi:10.1016/j.cardiores.2006.02.033 · Cited by 362
Self-citationNox4 Is Required for Maintenance of the Differentiated Vascular Smooth Muscle Cell Phenotype
Clempus, R. E., Sorescu, D., Dikalova, A., Pounkova, L., Jo, P. C., Sorescu, G. P., Lassegue, B. P., & Griendling, K. K. (2006) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/01.atv.0000251500.94478.18 · Cited by 338
Self-citationNox/Duox family of nicotinamide adenine dinucleotide (phosphate) oxidases
Lambeth, J. D. (2002) · Current Opinion in Hematology · doi:10.1097/00062752-200201000-00003 · Cited by 282
Barraclough, B. M. (1976) · The Lancet · doi:10.1016/s0140-6736(76)92691-x · Cited by 279
NOX5 NAD(P)H oxidase regulates growth and apoptosis in DU 145 prostate cancer cells
Brar, S. S., Corbin, Z. A., Kennedy, T. P., Hemendinger, R. A., Thornton, L., Bommarius, B., Arnold, R. S., Whorton, A. R., Sturrock, A., Huecksteadt, T. P., et al. (2003) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00525.2002 · Cited by 262
NOX5 variants are functionally active in endothelial cells
BelAiba, R. S., Djordjevic, T., Petry, A., Diemer, K., Bonello, S., Banfi, B., Hess, J., Pogrebniak, A., Bickel, C., & Görlach, A. (2007) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2006.10.054 · Cited by 255
Novel Mechanism of Activation of NADPH Oxidase 5
Jagnandan, D., Church, J. E., Banfi, B., Stuehr, D. J., Marrero, M. B., & Fulton, D. J. R. (2007) · Journal of Biological Chemistry · doi:10.1074/jbc.m608966200 · Cited by 210
Local Expression of Inflammatory Cytokines in Human Atherosclerotic Plaques
Wilcox, J. N., Nelken, N. A., Coughlin, S. R., Gordon, D., & Schall, T. J. (1994) · Journal of Atherosclerosis and Thrombosis · doi:10.5551/jat1994.1.supplemment1_s10 · Cited by 177
Sirois, M. G., Simons, M., & Edelman, E. R. (1997) · Circulation · doi:10.1161/01.cir.95.3.669 · Cited by 153
Fu, X., Beer, D. G., Behar, J., Wands, J. R., Lambeth, D. J., & Cao, W. (2006) · Journal of Biological Chemistry · doi:10.1074/jbc.m603353200 · Cited by 132
Expression and Activity of NOX5 in the Circulating Malignant B Cells of Hairy Cell Leukemia
Kamiguti, A. S., Serrander, L., Lin, K., Harris, R. J., Cawley, J. C., Allsup, D. J., Slupsky, J. R., Krause, K., & Zuzel, M. (2005) · The Journal of Immunology · doi:10.4049/jimmunol.175.12.8424 · Cited by 118
Role of the JAK-STAT pathway in PDGF-stimulated proliferation of human airway smooth muscle cells
Simon, A. R., Takahashi, S., Severgnini, M., Fanburg, B. L., & Cochran, B. (2002) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.00315.2001 · Cited by 115
NADPH oxidase 5 (NOX5) interacts with and is regulated by calmodulin
Tirone, F., & Cox, J. A. (2007) · FEBS Letters · doi:10.1016/j.febslet.2007.02.047 · Cited by 96
Kappert, K., Sparwel, J., Sandin, Å., Seiler, A., Siebolts, U., Leppänen, O., Rosenkranz, S., & Östman, A. (2006) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/01.atv.0000246777.30819.85 · Cited by 75
Masamune, A. (2005) · World Journal of Gastroenterology · doi:10.3748/wjg.v11.i22.3385 · Cited by 56
Human monocyte-endothelial cell interaction induces platelet-derived growth factor expression
Funayama, H., Ikeda, U., Takahashi, M., Sakata, Y., Kitagawa, S., Takahashi, Y., Masuyama, J., Furukawa, Y., Miura, Y., Kano, S., et al. (1998) · Cardiovascular Research · doi:10.1016/s0008-6363(97)00224-1 · Cited by 48
Rutherford, C. L., Martin, W., CARRIER, M. J., Änggård, E., & Ferns, G. A. (1997) · International Journal of Experimental Pathology · doi:10.1046/j.1365-2613.1997.d01-237.x · Cited by 39
Noiseux, N., Boucher, C. H., Cartier, R., & Sirois, M. G. (2000) · Circulation · doi:10.1161/01.cir.102.11.1330 · Cited by 33
Nie, L., Mogami, H., Kanzaki, M., Shibata, H., & Kojima, I. (1996) · Molecular Pharmacology · doi:10.1016/s0026-895x(25)09376-9 · Cited by 31
Lee, J. S., Kypreos, K. E., & Sonenshein, G. E. (1998) · Experimental Cell Research · doi:10.1006/excr.1997.3919 · Cited by 21
Lamb, D. J., Avades, T. Y., & Ferns, G. A. (2001) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/01.atv.21.6.997 · Cited by 21
Early PDGF-induced alterations in cytosolic free calcium are required for mitogenesis.
Diliberto, P. A., Hubbert, T., & Herman, B. A. (1991) · PubMed · Cited by 17
Seewald, S., Sachinidis, A., Seul, C., Kettenhofen, R. F., Ko, Y. D., & Vetter, H. (1997) · Journal of Hypertension · doi:10.1097/00004872-199715120-00071 · Cited by 14
Böhm, M., Blasius, S., & Roessner, A. (1994) · PubMed · Cited by 7
Cited by
Sorted by citations
Reactive Oxygen Species in Inflammation and Tissue Injury
Mittal, M., Siddiqui, M. R., Tran, K., Reddy, S. P., & Malik, A. B. (2014) · Antioxidants & Redox Signaling · doi:10.1089/ars.2012.5149 · Cited by 4,897
Combating oxidative stress in vascular disease: NADPH oxidases as therapeutic targets
Drummond, G. R., Selemidis, S., Griendling, K. K., & Sobey, C. G. (2011) · Nature Reviews Drug Discovery · doi:10.1038/nrd3403 · Cited by 899
Self-citationNox proteins in signal transduction
Brown, D., & Griendling, K. K. (2009) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2009.07.023 · Cited by 837
Self-citationBiochemistry, Physiology, and Pathophysiology of NADPH Oxidases in the Cardiovascular System
Lassegue, B. P., Martín, A. S., & Griendling, K. K. (2012) · Circulation Research · doi:10.1161/circresaha.111.243972 · Cited by 780
Self-citationMitochondrial dynamics, mitophagy and cardiovascular disease
Vásquez-Trincado, C., García‐Carvajal, I., Pennanen, C., Parra, V., Hill, J. A., Rothermel, B. A., & Lavandero, S. (2015) · The Journal of Physiology · doi:10.1113/jp271301 · Cited by 637
Metadata
- Authors
- Jay, D. B., Papaharalambus, C. A., Seidel-Rogol, B., Dikalova, A. E., Lassègue, B., & Griendling, K. K.
- Affiliations
- Emory University (US)
- Corresponding author
- Griendling, Kathy K.
- Venue
- Free Radical Biology and Medicine
- Publisher
- Elsevier BV
- Published
- 2008-08
- Volume
- 45
- Issue
- 3
- Pages
- 329-335
- ISSN
- 0891-5849 (ISSN-L)
- PMC
- PMC2459308
- OpenAlex
- W2049535510
- Cited by
- 166
- References
- 38
- Citations by year
- 2026: 1 · 2024: 4 · 2023: 4 · 2022: 9 · 2021: 3 · 2020: 6 · 2019: 9 · 2018: 12 · 2017: 8 · 2016: 2 · 2015: 12 · 2014: 15 · 2013: 18 · 2012: 18
- FWCI
- 10.16
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Free Radical Biology and Medicine (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health
- National Heart, Lung, and Blood Institute (HL058863, F32HL074604, HL074604, R01HL058863)
- Topics
- Health Sciences › Medicine › Physiology (medical) › Nitric Oxide and Endothelin Effects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Keywords
- NADPH oxidase 5, platelet-derived growth factor, smooth muscle cell proliferation, human aortic smooth muscle cells, reactive oxygen species, NADPH oxidase, JAK/STAT signaling, JAK2 phosphorylation, STAT3 phosphorylation, N-acetylcysteine, siRNA knockdown, vascular smooth muscle
- MeSH terms
- NADPH Oxidase 5, Aorta, Cells, Cultured, Enzyme Inhibitors, Humans, Membrane Proteins, Muscle, Smooth, Vascular, Phosphorylation, Platelet-Derived Growth Factor, Transfection, Blotting, Western, Reactive Oxygen Species, NADPH Oxidases, Protein Isoforms, Myocytes, Smooth Muscle, Cell Proliferation, STAT3 Transcription Factor, Janus Kinase 2
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
