The DOI matches its Crossref record; 53 of the 53 references it cites were found in a registry or an index.
Measuring the poise of thiol/disulfide couples in vivo
Open accessAbstract
The reduction potentials (Eh) for the redox couples GSH/GSSG and cysteine/cystine (Cys/CySS) in plasma are useful indicators of systemic oxidative stress and other medically relevant physiological states. This paper describes a sensitive method for determining plasma levels of GSH, GSSG, Cys and CySS used to calculate the in vivo Eh values. The method uses iodoacetate to alkylate free thiols; derivatization with dansyl chloride to fluorescently tag amino groups; and HPLC and fluorescence to separate, detect and quantify the molecules. Benefits of the method, such as sensitivity and dynamic range, are described; as are caveats, such as the importance of preventing red blood cell hemolysis and limitations in quantification of GSSG. General principles of redox chemistry and previous studies showing that the compounds are more oxidized than predicted from their standard reduction potentials are reviewed. The calculated in vivo reduction potential, Eh, is a convenient and informative way of summarizing the redox environment of plasma and is also useful for studies of cerebrospinal fluid, lymph, bronchoalveolar lavage fluid, human biopsies and a broad range of in vitro cell culture conditions.
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Cites
Tietze, F. (1969) · Analytical Biochemistry · doi:10.1016/0003-2697(69)90064-5 · Cited by 6,047
Schafer, F. Q., & Buettner, G. R. (2001) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(01)00480-4 · Cited by 4,587
A fluorometric method for determination of oxidized and reduced glutathione in tissues
Hissin, P. J., & Hilf, R. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90326-2 · Cited by 4,108
Jones, D. P. (2006) · Antioxidants & Redox Signaling · doi:10.1089/ars.2006.8.1865 · Cited by 1,726
Self-citationRadical-free biology of oxidative stress
Jones, D. P. (2008) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00283.2008 · Cited by 1,213
Self-citationReed, D. J., Babson, J. R., Beatty, P. W., Brodie, A. E., Ellis, W. W., & Potter, D. W. (1980) · Analytical Biochemistry · doi:10.1016/0003-2697(80)90118-9 · Cited by 1,177
Oxidation-reduction potentials of organic systems
(1960) · Journal of the Franklin Institute · doi:10.1016/0016-0032(60)90644-x · Cited by 882
[11] Redox potential of GSH/GSSG couple: Assay and biological significance
Jones, D. P. (2002) · Methods in Enzymology · doi:10.1016/s0076-6879(02)48630-2 · Cited by 812
Self-citationMolecular and Cellular Aspects of Thiol–Disulfide Exchange
Gilbert, H. F. (1990) · Advances in Enzymology - and Related Areas of Molecular Biology · doi:10.1002/9780470123096.ch2 · Cited by 758
A scheme for the colorimetric determination of microgram amounts of thiols
Saville, B. (1958) · The Analyst · doi:10.1039/an9588300670 · Cited by 750
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Free radicals and antioxidants: updating a personal view
Halliwell, B. (2012) · Nutrition Reviews · doi:10.1111/j.1753-4887.2012.00476.x · Cited by 912
Griendling, K. K., Touyz, R. M., Zweier, J. L., Dikalov, S., Chilian, W., Chen, Y.-R., Harrison, D. G., & Bhatnagar, A. (2016) · Circulation Research · doi:10.1161/res.0000000000000110 · Cited by 444
Welsh, J. L., Wagner, B. A., van’t Erve, T. J., Zehr, P. S., Berg, D. J., Halfdanarson, T. R., Yee, N. S., Bodeker, K. L., Du, J., Roberts, L. J., et al. (2013) · Cancer Chemotherapy and Pharmacology · doi:10.1007/s00280-013-2070-8 · Cited by 310
Thiol/disulfide redox states in signaling and sensing
Go, Y.-M., & Jones, D. P. (2013) · Critical Reviews in Biochemistry and Molecular Biology · doi:10.3109/10409238.2013.764840 · Cited by 241
Self-citationImplications of plasma thiol redox in disease
Oliveira, P. V. S., & Laurindo, F. R. M. (2018) · Clinical Science · doi:10.1042/cs20180157 · Cited by 127
Metadata
- Authors
- Jones, D. P., & Liang, Y.
- Venue
- Free Radical Biology and Medicine
- Publisher
- Elsevier BV
- Published
- 2009-11
- Volume
- 47
- Issue
- 10
- Pages
- 1329-1338
- ISSN
- 0891-5849 (ISSN-L)
- OpenAlex
- W2012879076
- Cited by
- 340
- References
- 53
- FWCI
- 4.20
- Type
- journal article
- Language
- en
- Open access
- green
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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