The StLAC2 gene is required for cell wall integrity, DHN-melanin synthesis and the pathogenicity of Setosphaeria turcica
Abstract
Laccases are blue multicopper oxidases, play important roles in various biological processes. These processes include fungal dihydroxynaphthalene (DHN)-melanin biosynthesis and pathogenicity, cellular growth, morphogenesis, and differentiation. This study investigated functions of the laccase gene StLAC2 in Setosphaeria turcica. The Δlac2 mutant colony color was distinct from that of the S. turcica wild-type (WT) isolate, and the mutants exhibited defective conidial formation. In contrast to the WT, the mutants exhibited a lighter color on the 2, 2-azino-di-[3-ethylbenzo-thia-zolin-sulphonate] (ABTS) plates, and the intracellular laccase activity was lower. Notably, StLAC2 gene loss correlated with decreased DHN-melanin biosynthesis and affected the integrity of the cell wall, where the StLAC2 gene mutants showed thinner, more transparent walls with a higher number of mitochondria than the WT. The Δlac2 mutants also lost their pathogenicity in maize. The results indicated that the StLAC2 gene involved in cell wall integrity, melanin biosynthesis and appressorial and conidial formation.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
StLAC2 participates in DHN-melanin synthesis, ensuring normal appressorial function, and is essential for maintaining cell wall integrity and pathogenicity.
“StLAC2, a member of the LAC-like multicopper oxidase family, plays a dual key role. It participates in DHN-melanin synthesis, ensuring normal appressorial function, and is essential for maintaining cell wall integrity and pathogenicity.”
From Fungal Melanin in Plant Pathogens: Complex Biosynthesis Pathways and Diverse Biological Functions (2025) · cites this paper as [60]
Cites
Hydrolytic Polyketide Shortening by Ayg1p, a Novel Enzyme Involved in Fungal Melanin Biosynthesis
Fujii, I., Yasuoka, Y., Tsai, H.-F., Chang, Y. C., Kwon-Chung, K. J., & Ebizuka, Y. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m406758200 · Cited by 105 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Fungal Melanin in Plant Pathogens: Complex Biosynthesis Pathways and Diverse Biological Functions
Jia, H., Liu, N., Zhang, L., Li, P., Meng, Y., Yuan, W., Li, H., Tantai, D., Qu, Q., Cao, Z., et al. (2025) · Plants · doi:10.3390/plants14142121 · Cited by 18 · see citing works
Self-citation
- Authors
- Ma, S., Cao, K., Liu, N., Meng, C., Cao, Z., Dai, D., Jia, H., Zang, J., Li, Z., Hao, Z., Gu, S., & Dong, J.
- Affiliations
- Hebei Agricultural University (CN)
- Hebei Academy of Agriculture and Forestry Sciences (CN)
- Corresponding author
- Cao, Zhiyan
- Dong, Jingao
- Venue
- Fungal Biology
- Publisher
- Elsevier BV
- Published
- 2017-06
- Volume
- 121
- Issue
- 6-7
- Pages
- 589-601
- ISSN
- 1878-6146 (ISSN-L)
- OpenAlex
- W2605596222
- Cited by
- 42
- References
- 1
- Citations by year
- 2026: 2 · 2025: 6 · 2024: 5 · 2023: 8 · 2022: 15 · 2021: 1 · 2020: 1 · 2019: 1 · 2018: 1 · 2017: 2
- FWCI
- 0.71
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Fungal Biology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural science Foundation of China (31101402)
- China Agriculture Research System (CARS-02-12)
- Research Fund for the Doctoral Program of Higher Education of China (20111302120004)
- Science and Technology Research project of Hebei Province (ZD2014053)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Plant Pathogens and Fungal Diseases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Health Sciences › Medicine › Epidemiology › Fungal Infections and Studies
- Keywords
- laccase, Setosphaeria turcica, cell wall integrity, fungal pathogenicity, conidial formation, appressorium formation
- MeSH terms
- Ascomycota, Cell Wall, Zea mays, Genes, Fungal, Melanins, Naphthols, Plant Diseases, Virulence, Gene Deletion, Laccase
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
