Cited by 35Sperm and Testicular FunctionReproductive biology and impacts on aquatic speciesDevelopmental Biology and Gene Regulation

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Gudu, an Armadillo repeat-containing protein, is required for spermatogenesis in Drosophila

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Cheng, Wei, Ip, Y. Tony, Xu, Zuoshang·2013·Gene

DOI 10.1016/j.gene.2013.08.080PMC PMC3839114

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Abstract

The Drosophila annotated gene CG5155 encodes a protein that contains 10 Armadillo-repeats and has an unknown function. To fill this gap, we performed loss-of-function studies using RNAi. By analysis of four independent Drosophila RNAi lines targeting two non-overlapping regions of the CG5155 transcript, we demonstrate that this gene is required for male fertility. Therefore, we have named this gene Gudu. The transcript of Gudu is highly enriched in adult testes. Knockdown of Gudu by a ubiquitous driver leads to defects in the formation of the individualization complex that is required for spermatid maturation, thereby impairing spermatogenesis. Furthermore, testis-specific knockdown of Gudu by crossing the RNAi lines with the bam-Gal4 driver is sufficient to cause the infertility and defective spermatogenesis. Since Gudu is highly homologous to vertebrate ARMC4, also an Armadillo-repeat-containing protein enriched in testes, our results suggest that Gudu and ARMC4 are a subfamily of Armadillo-repeat containing proteins that may have an evolutionarily conserved function in spermatogenesis.

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Authors: Cheng, W., Ip, Y. T., & Xu, Z.
Affiliations: University of Massachusetts Chan Medical School (US)
Corresponding author: Ip, Y. Tony
ORCID: Ip, Y. Tony
Xu, Zuoshang
Venue: Gene
Publisher: Elsevier BV
Published: 2013-12
Volume: 531
Issue: 2
Pages: 294-300
ISSN: 0378-1119 (ISSN-L)
DOI: 10.1016/j.gene.2013.08.080
PMC: PMC3839114
OpenAlex: W2082726323
Cited by: 35
References: 27
Citations by year: 2026: 2 · 2025: 1 · 2024: 3 · 2023: 5 · 2022: 7 · 2021: 7 · 2019: 2 · 2018: 2 · 2017: 2 · 2016: 2 · 2015: 1 · 2014: 1
FWCI: 1.62
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Gene (publishedVersion)
PubMed (publishedVersion)
PubMed Central (acceptedVersion)
The Journal of the American Medical Association (JAMA) Network (American Medical Association) (submittedVersion)
Funders: National Institute of Neurological Disorders and Stroke to ZX (5R01NS059708)
NIH (DK83450)
UMass DERC (DK32520)
UMass CCTS (UL1TR000161)
Guangdong Innovative Research Team Program (201001Y0104789252)
Topics: Health Sciences › Medicine › Reproductive Medicine › Sperm and Testicular Function
Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Reproductive biology and impacts on aquatic species
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
Keywords: armadillo repeats, spermatogenesis, male fertility, spermatid maturation, testis, Drosophila, RNAi knockdown, gene function
MeSH terms: Animals, Drosophila melanogaster, Female, Infertility, Male, Male, Meiosis, Spermatogenesis, Testis, Tissue Distribution, Drosophila Proteins, Animals, Genetically Modified, Armadillo Domain Proteins, Mice, Gene Knockdown Techniques

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Gudu, an Armadillo repeat-containing protein, is required for spermatogenesis in Drosophila

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