The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
Gudu, an Armadillo repeat-containing protein, is required for spermatogenesis in Drosophila
Open accessAbstract
The Drosophila annotated gene CG5155 encodes a protein that contains 10 Armadillo-repeats and has an unknown function. To fill this gap, we performed loss-of-function studies using RNAi. By analysis of four independent Drosophila RNAi lines targeting two non-overlapping regions of the CG5155 transcript, we demonstrate that this gene is required for male fertility. Therefore, we have named this gene Gudu. The transcript of Gudu is highly enriched in adult testes. Knockdown of Gudu by a ubiquitous driver leads to defects in the formation of the individualization complex that is required for spermatid maturation, thereby impairing spermatogenesis. Furthermore, testis-specific knockdown of Gudu by crossing the RNAi lines with the bam-Gal4 driver is sufficient to cause the infertility and defective spermatogenesis. Since Gudu is highly homologous to vertebrate ARMC4, also an Armadillo-repeat-containing protein enriched in testes, our results suggest that Gudu and ARMC4 are a subfamily of Armadillo-repeat containing proteins that may have an evolutionarily conserved function in spermatogenesis.
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“Interestingly, 5 of the genes identified in our assay are homologs of human PCD genes (Fig 3B), of which CG5155 (ARMC4) and CG31320 (DNAAF5) have since been shown to be required in Drosophila for male fertility [43,44].”
From Functional unknomics: Systematic screening of conserved genes of unknown function (2023) · cites this paper as [43]
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A survey of ovary-, testis-, and soma-biased gene expression in Drosophila melanogasteradults
Parisi, M. J., Nuttall, R., Edwards, P., Minor, J. M., Naiman, D. Q., Lü, J., Doctolero, M. H., Vainer, M., Cathy, C., Malley, J. D., et al. (2004) · Genome biology · doi:10.1186/gb-2004-5-6-r40 · Cited by 301
Armadillo-repeat protein functions: questions for little creatures
Tewari, R., Bailes, E., Bunting, K. A., & Coates, J. C. (2010) · Trends in Cell Biology · doi:10.1016/j.tcb.2010.05.003 · Cited by 271
Structural Basis of Rho GTPase-Mediated Activation of the Formin mDia1
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Genetic control of cell proliferation and differentiation inDrosophilaspermatogenesis
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Genetic dissection of sperm individualization in Drosophila melanogaster
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Shimada, K., Park, S., Miyata, H., Yu, Z., Morohoshi, A., Oura, S., Matzuk, M. M., & Ikawa, M. (2021) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2018355118 · Cited by 101
Functional unknomics: Systematic screening of conserved genes of unknown function
Rocha, J. J., Jayaram, S. A., Stevens, T. J., Muschalik, N., Shah, R. D., Emran, S., Robles, C., Freeman, M., & Munro, S. (2023) · PLOS Biology · doi:10.1371/journal.pbio.3002222 · Cited by 70
Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility
Lei, Y., Zhang, X., Xu, Q., Liu, S., Li, C., Jiang, H., Lin, H., Kong, E., Liu, J., Qi, S., et al. (2021) · Developmental Cell · doi:10.1016/j.devcel.2021.07.015 · Cited by 65
Separating from the pack: Molecular mechanisms ofDrosophilaspermatid individualization
Steinhauer, J. (2015) · Spermatogenesis · doi:10.1080/21565562.2015.1041345 · Cited by 62
Misra, S., Pandey, A. K., Gupta, S., Kumar, A., Khanna, P., Shankar, J., & Ram, K. R. (2017) · Scientific Reports · doi:10.1038/srep40372 · Cited by 56
Metadata
- Authors
- Cheng, W., Ip, Y. T., & Xu, Z.
- Affiliations
- University of Massachusetts Chan Medical School (US)
- Corresponding author
- Ip, Y. Tony
- Venue
- Gene
- Publisher
- Elsevier BV
- Published
- 2013-12
- Volume
- 531
- Issue
- 2
- Pages
- 294-300
- ISSN
- 0378-1119 (ISSN-L)
- PMC
- PMC3839114
- OpenAlex
- W2082726323
- Cited by
- 35
- References
- 27
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 3 · 2023: 5 · 2022: 7 · 2021: 7 · 2019: 2 · 2018: 2 · 2017: 2 · 2016: 2 · 2015: 1 · 2014: 1
- FWCI
- 1.62
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Gene (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- The Journal of the American Medical Association (JAMA) Network (American Medical Association) (submittedVersion)
- Funders
- National Institute of Neurological Disorders and Stroke to ZX (5R01NS059708)
- NIH (DK83450)
- UMass DERC (DK32520)
- UMass CCTS (UL1TR000161)
- Guangdong Innovative Research Team Program (201001Y0104789252)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Sperm and Testicular Function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Reproductive biology and impacts on aquatic species
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Keywords
- armadillo repeats, spermatogenesis, male fertility, spermatid maturation, testis, Drosophila, RNAi knockdown, gene function
- MeSH terms
- Animals, Drosophila melanogaster, Female, Infertility, Male, Male, Meiosis, Spermatogenesis, Testis, Tissue Distribution, Drosophila Proteins, Animals, Genetically Modified, Armadillo Domain Proteins, Mice, Gene Knockdown Techniques
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