Improved real-time PCR method for quantification of the abundance of all known ribotypes of the ichthyotoxic dinoflagellate Cochlodinium polykrikoides by comparing 4 different preparation methods.

Lee, Sung Yeon; Jeong, Hae Jin; Seong, Kyeong Ah; Lim, An Suk; Kim, Ji Hye; Lee, Kyung Ha; Lee, Moo Joon; Jang, Se Hyeon

doi:10.1016/j.hal.2017.01.006

Cited by 17Marine Toxins and Detection MethodsMolecular Biology Techniques and ApplicationsMarine Plankton and Biogeochemistry

The DOI matches its Crossref record; 39 of the 39 references it cites were found in a registry or an index.

Improved real-time PCR method for quantification of the abundance of all known ribotypes of the ichthyotoxic dinoflagellate Cochlodinium polykrikoides by comparing 4 different preparation methods.

Lee, Sung Yeon, Jeong, Hae Jin, Seong, Kyeong Ah, Lim, An Suk, Kim, Ji Hye, Lee, Kyung Ha, Lee, Moo Joon, Jang, Se Hyeon·2017·Harmful Algae

DOI 10.1016/j.hal.2017.01.006

Open this paper in CoChat — free account

Abstract

Red tides by the ichthyotoxic dinoflagellate Cochlodinium polykrikoides have caused large scaled mortality of fish and great loss in aquaculture industry in many countries. Detecting and quantifying the abundance of this species are the most critical step in minimizing the loss. The conventional quantitative real-time PCR (qPCR) method has been used for quantifying the abundance of this species. However, when analyzing >500 samples collected during huge C. polykrikoides red tides in South Sea of Korea in 2014, this conventional method and the previously developed specific primer and probe set for C. polykrikoides did not give reasonable abundances when compared with cell counting data. Thus improved qPCR methods and a new specific primer and probe set reflecting recent discovery of 2 new ribotypes have to be developed. A new species-specific primer and probe set for detecting all 3 ribotypes of C. polykrikoides was developed and provided in this study. Furthermore, because the standard curve between cell abundance and threshold cycle value (Ct) is critical, the efficiencies of 4 different preparation methods used to determine standard curves were comparatively evaluated. The standard curves were determined by using the following 4 different preparations: (1) extraction of DNA from a dense culture of C. polykrikoides followed by serial dilution of the extracted DNA (CDD method), (2) extraction of DNA from each of the serially diluted cultures with different concentrations of C. polykrikoides cultures (CCD method), (3) extraction of DNA from a dense field sample of C. polykrikoides collected from natural seawater and then dilution of the extracted DNA in serial (FDD method), and (4) extraction of DNA from each of the serially diluted field samples having different concentrations of C. polykrikoides (FCD method). These 4 methods yielded different results. The abundances of C. polykrikoides in the samples collected from the coastal waters of South Sea, Korea, in 2014-2015, obtained using the standard curves determined by the CCD and the FCD methods, were the most similar (0.93-1.03 times) and the second closest (1.16-1.33 times) to the actual cell abundances obtained by enumeration of cells. Thus, our results suggest that the CCD method is a more effective tool to quantify the abundance of C. polykrikoides than the conventional method, CDD, and the FDD and FCD methods.

Integrity signals

Registry

Crossref · 10.1016/j.hal.2017.01.006

Cites

39 of 39 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Numerous studies have shown the utility and statistical rigor of determining cell concentrations using qPCR compared to other visual detection methods for the HAB species presented here.
“Numerous studies have shown the utility and statistical rigor of determining cell concentrations using qPCR compared to other visual detection methods for the HAB species presented here (e.g., Handy et al., 2008;Eckford-Soper and Daugbjerg, 2015;Lee et al., 2017;Vandersea et al., 2017).”
From Current and Future Remote Sensing of Harmful Algal Blooms in the Chesapeake Bay to Support the Shellfish Industry (2020) · cites this paper as Lee et al., 2017

MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0
Tamura, K., Dudley, J. T., Nei, M., & Kumar, S. (2007) · Molecular Biology and Evolution · doi:10.1093/molbev/msm092 · Cited by 28,734
Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt, and Detonula confervacea (cleve) Gran.
Guillard, R. R., & Ryther, J. (1962) · Canadian Journal of Microbiology (print) · doi:10.1139/m62-029 · Cited by 7,663
A review of harmful algal blooms and their apparent global increase
Hallegraeff, G. M. (1993) · Phycologia · doi:10.2216/i0031-8884-32-2-79.1 · Cited by 2,466
OligoCalc: an online oligonucleotide properties calculator
Kibbe, W. A. (2007) · Nucleic Acids Research · doi:10.1093/nar/gkm234 · Cited by 1,349
Quantitative Analysis of Small-Subunit rRNA Genes in Mixed Microbial Populations via 5′-Nuclease Assays
Suzuki, M. T., Taylor, L. T., & DeLong, E. F. (2000) · Applied and Environmental Microbiology · doi:10.1128/aem.66.11.4605-4614.2000 · Cited by 1,194
IDENTIFICATION OF GROUP‐ AND STRAIN‐SPECIFIC GENETIC MARKERS FOR GLOBALLY DISTRIBUTED ALEXANDRIUM (DINOPHYCEAE). II. SEQUENCE ANALYSIS OF A FRAGMENT OF THE LSU rRNA GENE 1
Scholin, C. A., Herzog, M., Sogin, M. L., & Anderson, D. M. (1994) · Journal of Phycology · doi:10.1111/j.0022-3646.1994.00999.x · Cited by 887
Growth, feeding and ecological roles of the mixotrophic and heterotrophic dinoflagellates in marine planktonic food webs
Jeong, H. J., Yoo, Y. D., Kim, J. S., Seong, K. A., Kang, N. S., & Kim, T. (2010) · Ocean Science Journal · doi:10.1007/s12601-010-0007-2 · Cited by 647
Self-citation
Turning back the harmful red tide
Anderson, D. M. (1997) · Nature · doi:10.1038/41415 · Cited by 469
Development of a Real-Time PCR Assay for Rapid Detection and Quantification ofAlexandrium minutum(a Dinoflagellate)
Galluzzi, L., Penna, A., Bertozzini, E., Vila, M., Garcés, E., & Magnani, M. (2004) · Applied and Environmental Microbiology · doi:10.1128/aem.70.2.1199-1206.2004 · Cited by 282
Development of Real-Time PCR Assays for Rapid Detection of Pfiesteria piscicida and Related Dinoflagellates
Bowers, H. A., Tengs, T., Glasgow, H. B., Burkholder, J. M., Rublee, P. A., & Oldach, D. (2000) · Applied and Environmental Microbiology · doi:10.1128/aem.66.11.4641-4648.2000 · Cited by 231
Serious overestimation in quantitative PCR by circular (supercoiled) plasmid standard: microalgal pcna as the model gene
Hou, Y., Zhang, H., Miranda, L., & Lin, S. (2010) · PLoS ONE · doi:10.1371/journal.pone.0009545 · Cited by 222
Economic impact, management and mitigation of red tides in Korea
Park, T.-G., Lim, W., Park, Y. T., Lee, C. K., & Jeong, H. J. (2013) · Harmful Algae · doi:10.1016/j.hal.2013.10.012 · Cited by 191
Self-citation
Harmful dinoflagellate blooms caused by Cochlodinium sp.: Global expansion and ecological strategies facilitating bloom formation.
Kudela, R. M., & Gobler, C. J. (2012) · Harmful Algae · doi:10.1016/j.hal.2011.10.015 · Cited by 184
Improved quantitative real‐time PCR assays for enumeration of harmful algal species in field samples using an exogenous DNA reference standard
Coyne, K. J., Handy, S. M., Demir‐Hilton, E., Whereat, E. B., Hutchins, D. A., Portune, K. J., Doblin, M. A., & Cary, S. C. (2005) · Limnology and Oceanography Methods · doi:10.4319/lom.2005.3.381 · Cited by 158
IDENTIFICATION OF PFIESTERIA PISCICIDA (DINOPHYCEAE) AND PFIESTERIA‐LIKE ORGANISMS USING INTERNAL TRANSCRIBED SPACER‐SPECIFIC PCR ASSAYS1
Litaker, R. W., Vandersea, M. W., Kibler, S. R., Reece, K. S., Stokes, N. A., Steidinger, K. A., Millie, D. F., Bendis, B., Pigg, R. J., & Tester, P. A. (2003) · Journal of Phycology · doi:10.1046/j.1529-8817.2003.02112.x · Cited by 138
Monitoring and trends in harmful algal blooms and red tides in Korean coastal waters, with emphasis on Cochlodinium polykrikoides
Lee, C.-K., Park, T.-G., Park, Y.-T., & Lim, W.-A. (2013) · Harmful Algae · doi:10.1016/j.hal.2013.10.002 · Cited by 137
Analysis of rRNA gene content in the Mediterranean dinoflagellate Alexandrium catenella and Alexandrium taylori: implications for the quantitative real-time PCR-based monitoring methods
Galluzzi, L., Bertozzini, E., Penna, A., Perini, F., Garcés, E., & Magnani, M. (2009) · Journal of Applied Phycology · doi:10.1007/s10811-009-9411-3 · Cited by 129
Characterization of the toxicity of Cochlodinium polykrikoides isolates from Northeast US estuaries to finfish and shellfish.
Tang, Y. Z., & Gobler, C. J. (2009) · Harmful Algae · doi:10.1016/j.hal.2008.10.001 · Cited by 122
Phylogenetic relationships in the harmful dinoflagellate Cochlodinium polykrikoides (Gymnodiniales, Dinophyceae) inferred from LSU rDNA sequences
Iwataki, M., Kawami, H., Mizushima, K., Mikulski, C. M., Doucette, G. J., Relox, J. R., Anton, A., Fukuyo, Y., & Matsuoka, K. (2008) · Harmful Algae · doi:10.1016/j.hal.2007.12.003 · Cited by 112
The quantitative real-time PCR applications in the monitoring of marine harmful algal bloom (HAB) species
Penna, A., & Galluzzi, L. (2012) · Environmental Science and Pollution Research · doi:10.1007/s11356-012-1377-z · Cited by 108
Real-Time Quantitative PCR for Assessment of Abundance of Pseudoalteromonas Species in Marine Samples
Skovhus, T. L., Ramsing, N. B., Holmström, C., Kjelleberg, S. J., & Dahllöf, I. (2004) · Applied and Environmental Microbiology · doi:10.1128/aem.70.4.2373-2382.2004 · Cited by 108
Mixotrophic dinoflagellate red tides in Korean waters: Distribution and ecophysiology
Park, J., Jeong, H. J., Yoo, Y. D., & Yoon, E. Y. (2013) · Harmful Algae · doi:10.1016/j.hal.2013.10.004 · Cited by 92
Self-citation
Molecular quantification of toxic Alexandrium fundyense in the Gulf of Maine using real-time PCR
Dyhrman, S. T., Erdner, D. L., Du, J. K. L., Galac, M. R., & Anderson, D. M. (2005) · Harmful Algae · doi:10.1016/j.hal.2005.07.005 · Cited by 91
Red tides in Masan Bay, Korea in 2004–2005: I. Daily variations in the abundance of red-tide organisms and environmental factors
Jeong, H. J., Yoo, Y. D., Lee, K. H., Kim, T., Seong, K. A., Kang, N. S., Lee, S. Y., Kim, J. S., Kim, S., & Yih, W. (2013) · Harmful Algae · doi:10.1016/j.hal.2013.10.008 · Cited by 90
Self-citation
Molecular Detection and Quantitation of the Red TideDinoflagellate Karenia brevis in the MarineEnvironment
Gray, M., Wawrik, B., Paul, J. H., & Casper, E. T. (2003) · Applied and Environmental Microbiology · doi:10.1128/aem.69.9.5726-5730.2003 · Cited by 76
High intraindividual, intraspecific, and interspecific variability in large-subunit ribosomal DNA in the heterotrophic dinoflagellates Protoperidinium, Diplopsalis, and Preperidinium (Dinophyceae)
Gribble, K. E., & Anderson, D. M. (2007) · Phycologia · doi:10.2216/06-68.1 · Cited by 67
Use of Quantitative Real-Time PCR to Investigate the Dynamics of the Red Tide Dinoflagellate Lingulodinium polyedrum
Moorthi, S. D., Countway, P. D., Stauffer, B., & Caron, D. A. (2006) · Microbial Ecology · doi:10.1007/s00248-006-9030-3 · Cited by 60
Erratum to: The quantitative real-time PCR applications in the monitoring of marine harmful algal bloom (HAB) species
Penna, A., & Galluzzi, L. (2013) · Environmental Science and Pollution Research · doi:10.1007/s11356-013-1519-y · Cited by 59
Development of a Real-Time PCR Probe for Quantification of the Heterotrophic Dinoflagellate Cryptoperidiniopsis brodyi (Dinophyceae) in Environmental Samples
Park, T.-G., de Salas, M. F., Bolch, C. J. S., & Hallegraeff, G. M. (2007) · Applied and Environmental Microbiology · doi:10.1128/aem.02389-06 · Cited by 56
Inhibition of growth rate and swimming speed of the harmful dinoflagellate Cochlodinium polykrikoides by diatoms: Implications for red tide formation
Lim, A. S., Jeong, H. J., Jang, T. Y., Jang, S. H., & Franks, P. J. S. (2014) · Harmful Algae · doi:10.1016/j.hal.2014.05.003 · Cited by 51
Self-citation
Resolving the intra-specific succession within Cochlodinium polykrikoides populations in southern Korean coastal waters via use of quantitative PCR assays
Park, B. S., Wang, P., Kim, J. H., Kim, J., Gobler, C. J., & Han, M. (2014) · Harmful Algae · doi:10.1016/j.hal.2014.04.019 · Cited by 46
Self-citation
Monitoring toxic Ostreopsis cf. ovata in recreational waters using a qPCR based assay
Casabianca, S., Perini, F., Casabianca, A., Battocchi, C., Giussani, V., Chiantore, M., & Penna, A. (2014) · Marine Pollution Bulletin · doi:10.1016/j.marpolbul.2014.09.018 · Cited by 39
Development and field application of rRNA-targeted probes for the detection of Cochlodinium polykrikoides Margalef in Korean coastal waters using whole cell and sandwich hybridization formats
Mikulski, C. M., Park, Y. T., Jones, K. L., Lee, C. K., Lim, W., Lee, Y. S., Scholin, C. A., & Doucette, G. J. (2008) · Harmful Algae · doi:10.1016/j.hal.2007.12.015 · Cited by 39
Rapid and Accurate Identification by Real-Time PCR of Biotoxin-Producing Dinoflagellates from the Family Gymnodiniaceae
Smith, K. F., de Salas, M. F., Adamson, J. E., & Rhodes, L. (2014) · Marine Drugs · doi:10.3390/md12031361 · Cited by 38
Detection of Cochlodinium polykrikoides and Gymnodinium impudicum (Dinophyceae) in sediment samples from Korea using real-time PCR
Park, T.-G., & Park, Y. (2009) · Harmful Algae · doi:10.1016/j.hal.2009.08.002 · Cited by 29
Development of a SYTO9 based real-time PCR probe for detection and quantification of toxic dinoflagellateKarlodinium veneficum(Dinophyceae) in environmental samples
Park, T.-G., Park, Y., & Lee, Y. (2009) · Phycologia · doi:10.2216/08-52.1 · Cited by 27
Identification of the dinoflagellate community during Cochlodinium polykrikoides (Dinophyceae) blooms using amplified rDNA melting curve analysis and real-time PCR probes
Park, T.-G., Park, G.-H., Park, Y., Kang, Y.-S., Bae, H.-M., Kim, C., Jeong, H. J., & Lee, Y. (2008) · Harmful Algae · doi:10.1016/j.hal.2008.09.003 · Cited by 26
Self-citation
Rapid detection and quantification of Prorocentrum minimum by loop-mediated isothermal amplification and real-time fluorescence quantitative PCR
Zhang, F., Shi, Y., Jiang, K., Song, W., Ma, C., Xu, Z., & Ma, L. (2013) · Journal of Applied Phycology · doi:10.1007/s10811-013-0163-8 · Cited by 23
The Outbreak of Red Tides in the Coastal Waters off Kohung, Chonnam, Korea 2. The Temporal and Spatial Variations in the Phytoplanktonic Community in 1997
Jeong, H., Park, J.-K., Choi, H.-Y., Yang, J. S., Shim, J.-H., Shin, Y.-K., Yih, W., Kim, H.-S., & Cho, H. J. (2000) · Symposium on Experimental and Efficient Algorithms · Cited by 13
Self-citation

Flagged first, then by citations

Sorted by citations

Current and Future Remote Sensing of Harmful Algal Blooms in the Chesapeake Bay to Support the Shellfish Industry
Wolny, J. L., Tomlinson, M. C., Schollaert Uz, S., Egerton, T. A., McKay, J. R., Meredith, A., Reece, K. S., Scott, G. P., & Stumpf, R. P. (2020) · Frontiers in Marine Science · doi:10.3389/fmars.2020.00337 · Cited by 80
An advanced tool, droplet digital PCR (ddPCR), for absolute quantification of the red-tide dinoflagellate, Cochlodinium polykrikoides Margalef (Dinophyceae)
Lee, H.-G., Kim, H. M., Min, J., Kim, K., Park, M. G., Jeong, H. J., & Kim, K. Y. (2017) · ALGAE · doi:10.4490/algae.2017.32.9.10 · Cited by 26
Self-citation
Feeding by the heterotrophic nanoflagellate Katablepharis remigera on algal prey and its nationwide distribution in Korea
Ok, J. H., Jeong, H. J., Lim, A. S., Lee, S. Y., & Kim, S. J. (2018) · Harmful Algae · doi:10.1016/j.hal.2018.03.011 · Cited by 24
Self-citation
Comparison of the spatial-temporal distributions of the heterotrophic dinoflagellates Gyrodinium dominans, G. jinhaense, and G. moestrupii in Korean coastal waters
Lee, S. Y., Jeong, H. J., Kang, H. C., You, J. H., You, J. H., Park, S. A., & Eom, S. H. (2021) · ALGAE · doi:10.4490/algae.2021.36.3.4 · Cited by 21
Self-citation
Identification of ichthyotoxic red tide algae based on three-dimensional fluorescence spectra and particle swarm optimization support vector machine
Wang, S.-Y., Bi, W., Gan, W., Li, X., Zhang, B., Fu, G., & Jiang, T. (2021) · Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy · doi:10.1016/j.saa.2021.120711 · Cited by 19
Spatial and seasonal distributions of the phototrophic dinoflagellate Biecheleriopsis adriatica (Suessiaceae) in Korea: quantification using qPCR
Kang, H. C., Jeong, H. J., You, J. H., You, J. H., Jang, S. H., Lee, K. H., Lee, K. H., Park, J. Y., & Rho, J. (2019) · ALGAE · doi:10.4490/algae.2019.34.5.25 · Cited by 18
Self-citation
Spatio-temporal distributions of the newly described mixotrophic dinoflagellate Yihiella yeosuensis (Suessiaceae) in Korean coastal waters and its grazing impact on prey populations
Jang, S. H., & Jeong, H. J. (2020) · ALGAE · doi:10.4490/algae.2020.35.2.24 · Cited by 17
Self-citation
Newly discovered role of the heterotrophic nanoflagellate Katablepharis japonica, a predator of toxic or harmful dinoflagellates and raphidophytes
Kwon, J. E., Jeong, H. J., Kim, S. J., Jang, S. H., Lee, K. H., & Seong, K. A. (2017) · Harmful Algae · doi:10.1016/j.hal.2017.08.009 · Cited by 16
Self-citation
Spatial-temporal distributions of the newly described mixotrophic dinoflagellate Gymnodinium smaydae in Korean coastal waters
Lee, S. Y., Jeong, H. J., Ok, J. H., Kang, H. C., & You, J. H. (2020) · ALGAE · doi:10.4490/algae.2020.35.8.25 · Cited by 15
Self-citation
Rapid and sensitive detection of Karlodinium veneficum by a novel double-nick rolling circle amplification
Liu, F., Zhang, C., Zheng, H., Yang, Y., Zang, D., Wang, Y., & Chen, G. (2021) · Environmental Science and Pollution Research · doi:10.1007/s11356-021-13673-4 · Cited by 13
First report of the photosynthetic dinoflagellate Heterocapsa minima in the Pacific Ocean: morphological and genetic characterizations and the nationwide distribution in Korea
Lee, S. Y., Jeong, H. J., Kwon, J. E., You, J. H., Kim, S. J., Ok, J. H., Kang, H. C., & Park, J. Y. (2019) · ALGAE · doi:10.4490/algae.2019.34.2.28 · Cited by 13
Self-citation
Morphological and genetic characterization and the nationwide distribution of the phototrophic dinoflagellate Scrippsiella lachrymosa in the Korean waters
Lee, S. Y., Jeong, H. J., You, J. H., & Kim, S. J. (2018) · ALGAE · doi:10.4490/algae.2018.33.3.4 · Cited by 12
Self-citation
Effects of temperature on the autotrophic and mixotrophic growth rates of the dinoflagellate Biecheleria cincta and its spatiotemporal distributions under current temperature and global warming conditions
You, J. H., Jeong, H. J., Ok, J. H., Kang, H. C., Park, S. A., Eom, S. H., Lee, S. Y., & Kang, N. S. (2023) · Marine Biology · doi:10.1007/s00227-022-04167-z · Cited by 6
Self-citation
Propidium Monoazide based selective iDNA monitoring method improves eDNA monitoring for harmful algal bloom Alexandrium species
Yun, K.-W., Son, H.-S., Seong, M.-J., & Kim, M. (2023) · Frontiers in Marine Science · doi:10.3389/fmars.2023.1257343 · Cited by 4
Insights into plankton communities in a Margalefidinium polykrikoides (Gymnodiniales, Dinophyceae) bloom and adjacent non-bloom waters
Yang, H. J., Kim, Y. H., Seo, H. J., Lee, M. J., & Jang, S. H. (2024) · ALGAE · doi:10.4490/algae.2024.39.12.10 · Cited by 3
Self-citation
Harbouring Aliens: Ports as Habitats for Non-native Species
HOLMES, S. J. (2021) · Cronfa (Swansea University) · doi:10.23889/suthesis.58749
Standardization of A Method for the Detection of the Genus Prorocentrum, A Dinoflagellate Present in Harmful Algae Blooms (habs) in San Jorge Antofagasta Bay by Digital Droplet Pcr (ddPCR)
Camerón, H., Fernandez, D., Riquelme, C. E., & Vera-Villalobos, H. (2026) · Marine Biotechnology · doi:10.1007/s10126-026-10575-9

Quantitative Real-Time Polymerase Chain Reaction (PCR) Assay for Rapid Monitoring of the Harmful Algal Bloom Species Cochlodinium polykrikoides
Kim, M.-J., Kim, H., Park, J. S., Kang, D., Cho, S.-H., Kim, H., Baek, S. H., Park, J. J. C., Han, J., Kim, K. E., et al. (2025) · Journal of Marine Science and Engineering · doi:10.3390/jmse13020277 · Cited by 4
Rapid detection and Quantification of Fish Killing Dinoflagellate Cochlodinium polykrikoides (Dinophyceae) in Environmental Samples Using Real-time PCR
Park, T.-G., Kang, Y.-S., Seo, M.-K., Kim, C., & Park, Y. (2008) · Fisheries and aquatic sciences · doi:10.5657/fas.2008.11.4.205 · Cited by 1
An advanced tool, droplet digital PCR (ddPCR), for absolute quantification of the red-tide dinoflagellate, Cochlodinium polykrikoides Margalef (Dinophyceae)
Lee, H.-G., Kim, H. M., Min, J., Kim, K., Park, M. G., Jeong, H. J., & Kim, K. Y. (2017) · ALGAE · doi:10.4490/algae.2017.32.9.10 · Cited by 26
Self-citation
Use of Quantitative Real-Time PCR to Investigate the Dynamics of the Red Tide Dinoflagellate Lingulodinium polyedrum
Moorthi, S. D., Countway, P. D., Stauffer, B., & Caron, D. A. (2006) · Microbial Ecology · doi:10.1007/s00248-006-9030-3 · Cited by 60
Use of Molecular Detection Technique for Red Tide Warning of Cochlodinium polykrikoides
Park, T.-G., Won, K.-M., & Kim, W. J. (2016) · The Sea · doi:10.7850/jkso.2016.21.1.44

Authors: Lee, S. Y., Jeong, H. J., Seong, K. A., Lim, A. S., Kim, J. H., Lee, K. H., Lee, M. J., & Jang, S. H.
Affiliations: Seoul National University (KR)
Kunsan National University (KR)
Corresponding author: Lee, Sung Yeon
Jeong, Hae Jin
ORCID: Lee, Sung Yeon
Jeong, Hae Jin
Lim, An Suk
Kim, Ji Hye
Lee, Kyung Ha
Lee, Moo Joon
Jang, Se Hyeon
Venue: Harmful Algae
Publisher: Elsevier BV
Published: 2017-03
Volume: 63
Pages: 23-31
ISSN: 1568-9883 (ISSN-L)
DOI: 10.1016/j.hal.2017.01.006
OpenAlex: W2581941139
Cited by: 17
References: 39
Citations by year: 2026: 1 · 2024: 1 · 2023: 2 · 2021: 4 · 2020: 3 · 2019: 2 · 2018: 2 · 2017: 2
FWCI: 1.45
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Harmful Algae (publishedVersion)
PubMed (publishedVersion)
Seoul National University Open Repository (Seoul National University) (submittedVersion)
Funders: National Research Foundation of Korea
Korea government (NRF-2015-M1A5A1041806)
Program of Korea Institute of Marine Science and Technology Promotion
Topics: Physical Sciences › Environmental Science › Environmental Chemistry › Marine Toxins and Detection Methods
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
Physical Sciences › Earth and Planetary Sciences › Oceanography › Marine Plankton and Biogeochemistry
Keywords: Cochlodinium polykrikoides, red tide, ribotypes, quantitative real-time PCR, DNA extraction methods, fish mortality, aquaculture losses, South Sea of Korea
MeSH terms: Dinoflagellida, Environmental Monitoring, Ribotyping, Harmful Algal Bloom, Real-Time Polymerase Chain Reaction

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Improved real-time PCR method for quantification of the abundance of all known ribotypes of the ichthyotoxic dinoflagellate Cochlodinium polykrikoides by comparing 4 different preparation methods.

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks