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Poly-β-hydroxyalkanoates production from cassava starch hydrolysate by Cupriavidus sp. KKU38
Abstract
A bacterium capable of accumulating polyhydroxyalkanoates (PHAs), Cupriavidus sp. KKU38 (GenBank Accession no. AM260479), was isolated from cassava starch wastewater of cassava starch manufacturing process. KKU38 can utilize glucose, fructose, maltose and xylose for PHA production. Glucose was the most suitable sugar for PHA production giving the highest PHA content and yield of 73.88% and 0.16 g/g-total sugar consumed, respectively. Lactose and sucrose were not suitable carbon sources for both biomass and PHA productions. PHA production from cassava starch hydrolysate (CSH) by the strain KKU38 was maximized under N-limited conditions (COD:N:P of 100:1:2.43). The moderately high biomass concentrations of 5.97 g/L with PHA content and yield of 61.60% and 0.20 g/g-total sugar consumed, respectively, were obtained under the optimum conditions. The analysis of PHA produced under the optimum conditions by (1)H NMR, (13)C NMR and FT-IR indicated that the produced polymer is homopolymer polyhydroxybutyrate (PHB).
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“while 61.5% were achieved from cassava starch by Cupriavidus sp. KKU38 strain [105]”
From Food Waste Biorefinery: Pathway towards Circular Bioeconomy (2021) · cites this paper as [105]
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Metadata
- Authors
- Poomipuk, N., Reungsang, A., & Plangklang, P.
- Affiliations
- Khon Kaen University (TH)
- Corresponding author
- Reungsang, Alissara
- Venue
- International Journal of Biological Macromolecules
- Publisher
- Elsevier BV
- Published
- 2014-04
- Volume
- 65
- Pages
- 51-64
- ISSN
- 0141-8130 (ISSN-L)
- OpenAlex
- W2066371418
- Cited by
- 113
- References
- 56
- Citations by year
- 2025: 11 · 2024: 12 · 2023: 16 · 2022: 15 · 2021: 18 · 2020: 16 · 2019: 8 · 2018: 5 · 2017: 6 · 2016: 1 · 2015: 4 · 2014: 1
- FWCI
- 1.52
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- International Journal of Biological Macromolecules (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Office of National Research Council of Thailand, Bangkok, Thailand
- Fermentation Research Center for Value Added Agricultural Products, Khon Kaen University, Khon Kaen, Thailand
- Topics
- Physical Sciences › Materials Science › Biomaterials › biodegradable polymer synthesis and properties
- Life Sciences › Immunology and Microbiology › Applied Microbiology and Biotechnology › Biopolymer Synthesis and Applications
- Physical Sciences › Environmental Science › Pollution › Microbial bioremediation and biosurfactants
- Keywords
- polyhydroxyalkanoates, polyhydroxybutyrate, PHA production, cassava starch wastewater, glucose utilization, xylose utilization, nitrogen limitation, carbon source screening, PHA yield, biopolymer characterization
- MeSH terms
- Bacterial Proteins, Manihot, Feasibility Studies, Genotype, Hydrolysis, Nitrogen, Phenotype, Phosphorus, RNA, Ribosomal, 16S, Sewage, Species Specificity, Starch, Cupriavidus, Polyhydroxyalkanoates
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