CoChat
Sign Up
Cited by 86

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Cellular uptake and transport characteristics of chitosan modified nanoparticles in Caco-2 cell monolayers

Dou, Taoyan, Wang, Jie, Han, Chengkun, Shao, Xiaowei, Zhang, Jie, Lu, Wen·2019·International Journal of Biological Macromolecules
DOI 10.1016/j.ijbiomac.2019.07.168
Open this paper in CoChat — free account

Abstract

The bioadhesive drug delivery systems, chitosan modified nanoparticles, can efficiently adhere to mucosal surface of gastrointestinal tract and prolong residence time but a deeper understanding about its cellular uptake and transport is still lack. Hence, the present study was designed to compare the process of uptake and transport between chitosan modified and unmodified PLGA nanoparticles. Chitosan modified nanoparticles were formulated by classic and chemical methods. The morphology and modification potency were characterized by zeta potential, FTIR, DSC, XPS and TEM. Coumarin-6 load chitosan modified nanoparticles were incubated with Caco-2 cells to study the process of uptake and transport. The kinetic of cellular uptake for chitosan modified and unmodified nanoparticles was time- and concentration-dependent endocytosis but the cellular uptake efficiency of modified nanoparticles was significantly higher than that of unmodified one within the high concentration range (25.0-100.0 μg/mL). The modified nanoparticles underwent clathrin-mediated endocytosis and macropinocytosis but unmodified nanoparticles occurred a clathrin-mediated endocytosis. Both types of nanoparticles can increase caulophine with low solubility and poor stability transmembrane transport and the modified nanoparticles exhibited a better transcellular permeability. Therefore, the chitosan modified nanoparticles showed significantly improved cellular uptake and transcellular transport and a potential impr…

Overview

Integrity signals

Registry

Crossref · 10.1016/j.ijbiomac.2019.07.168

Cites

1 of 1 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Show all 1 references

Research works printed but not linked: 34

  1. Truong-Le, 2015, Stabilization challenges and formulation strategies associated with oral biologic drug delivery systems, Adv. Drug Deliv. Rev., 93, 95, 10.1016/j.addr.2015.08.001 · DOI
  2. Wang, 2016, In vitro and in vivo evaluation of functionalized chitosan-pluronic micelles loaded with myricetin on glioblastoma cancer, Nanomedicine, 12, 1263, 10.1016/j.nano.2016.02.004 · DOI
  3. Hu, 2018, Recent advances of polysaccharide-based nanoparticles for oral insulin delivery, Int. J. Biol. Macromol., 120, 775, 10.1016/j.ijbiomac.2018.08.152 · DOI
  4. Mukhopadhyay, 2018, Preparation, characterization and in vivo evaluation of pH sensitive, safe quercetin-succinylated chitosan-alginate core-shell-corona nanoparticle for diabetes treatment, Carbohydr. Polym., 182, 42, 10.1016/j.carbpol.2017.10.098 · DOI
  5. Jaruszewski, 2012, Chitosan enhances the stability and targeting of immuno-nanovehicles to cerebrovascular deposits of Alzheimer's disease amyloid protein, Nanomedicine, 8, 250, 10.1016/j.nano.2011.06.008 · DOI
  6. Rahbar, 2017, One-step synthesis of zirconia and magnetite nanocomposite immobilized chitosan for micro-solid-phase extraction of organophosphorous pesticides from juice and water samples prior to gas chromatography/mass spectroscopy, Food Anal. Methods, 10, 2229, 10.1007/s12161-016-0769-y · DOI
  7. Jana, 2015, Characterization of physicochemical and thermal properties of chitosan and sodium alginate after biofield treatment, Pharm. Anal. Acta, 6, 430, 10.4172/2153-2435.1000430 · DOI
  8. Walke, 2018, Preparation and characterization of microencapsulated DwPT trivalent vaccine using water soluble chitosan and its in-vitro and in-vivo immunological properties, Int. J. Biol. Macromol., 107, 2044, 10.1016/j.ijbiomac.2017.10.073 · DOI
  9. Yeh, 2011, Mechanism and consequence of chitosan-mediated reversible epithelial tight junction opening, Biomaterials, 32, 6164, 10.1016/j.biomaterials.2011.03.056 · DOI
  10. Kofuji, 2003, Retention and release behavior of insulin in chitosan gel beads, J. Biomater. Sci. Polym. Ed., 14, 1243, 10.1163/156856203322553464 · DOI
  11. Nafee, 2009, Relevance of the colloidal stability of chitosan/PLGA nanoparticles on their cytotoxicity profile, Int. J. Pharm., 381, 130, 10.1016/j.ijpharm.2009.04.049 · DOI
  12. Si, 2010, Effects of caulophine on caffeine-induced cellular injury and calcium homeostasis in rat cardiomyocytes, Basic Clin. Pharmacol. Toxicol., 107, 976, 10.1111/j.1742-7843.2010.00618.x · DOI
  13. Lu, 2018, Liquid chromatography coupled with hybrid ion trap time-of-flight mass spectrometry method for the determination of caulophine in rat bio-samples and its pharmacokinetic study after intragastric and intraperitoneal administration, J. Sep. Sci., 41, 422, 10.1002/jssc.201700846 · DOI
  14. Wen, 2010, Development and validation of a solid phase extraction and LC-MS method for the determination of caulophine in rat plasma and tissue: application to study its pharmacokinetics, Anal. Lett., 43, 2872, 10.1080/00032711003763681 · DOI
  15. Shi, 2015, Preparation and cellular targeting study of VEGF-conjugated PLGA nanoparticles, J. Microencapsul., 32, 699, 10.3109/02652048.2015.1035683 · DOI
  16. Zhao, 2018, Design and development of graphene oxide nanoparticle/chitosan hybrids showing pH-sensitive surface charge-reversible ability for efficient intracellular doxorubicin delivery, ACS Appl. Mater. Interfaces, 10, 6608, 10.1021/acsami.7b16910 · DOI
  17. Kim, 2015, Humidity-dependent compression-induced glass transition of the air–water interfacial Langmuir films of poly(D,L-lactic acid-ran-glycolic acid) (PLGA), Soft Matter, 11, 5666, 10.1039/C4SM02535K · DOI
  18. Duarte, 2017, Chitosan nanoparticles as a modified diclofenac drug release system, J. Nanopart. Res., 19, 274, 10.1007/s11051-017-3968-6 · DOI
  19. Goyal, 2016, Chitosan as a potential stabilizing agent for titania nanoparticle dispersions for preparation of multifunctional cotton fabric, Carbohydr. Polym., 154, 167, 10.1016/j.carbpol.2016.08.035 · DOI
  20. Aktaş, 2005, Preparation and in vitro evaluation of chitosan nanoparticles containing a caspase inhibitor, Int. J. Pharm., 298, 378, 10.1016/j.ijpharm.2005.03.027 · DOI
  21. Zhang, 2017, Homing in on an intracellular target for delivery of loaded nanoparticles unctionalized with a histone deacetylase inhibitor, Oncotarget, 8, 68242, 10.18632/oncotarget.20021 · DOI
  22. Chronopoulou, 2016, Chitosan based nanoparticles functionalized with peptidomimetic derivatives for oral drug delivery, New Biotechnol., 33, 23, 10.1016/j.nbt.2015.07.005 · DOI
  23. Hsu, 2002, Influence of ionic strength and pH on hydrophobicity and zeta potential of Giardia and Cryptosporidium, Colloids Surf. A Physicochem. Eng. Asp., 201, 201, 10.1016/S0927-7757(01)01009-3 · DOI
  24. Ibitoye, 2018, Extraction and physicochemical characterization of chitin and chitosan isolated from house cricket, Biomed. Mater., 13, 10.1088/1748-605X/aa9dde · DOI
  25. Casettari, 2011, Surface characterisation of bioadhesive PLGA/chitosan microparticles produced by supercritical fluid technology, Pharm. Res., 28, 1668, 10.1007/s11095-011-0403-z · DOI
  26. Zhang, 2018, Simple fabrication of chitosan/graphene nanoplates composite spheres for efficient adsorption of acid dyes from aqueous solution, Int. J. Biol. Macromol., 112, 1048, 10.1016/j.ijbiomac.2018.02.074 · DOI
  27. Peppas, 1985, Surface, interfacial and molecular aspects of polymer bioadhesion on soft tissues, J. Control. Release, 2, 257, 10.1016/0168-3659(85)90050-1 · DOI
  28. Wei, 2010, Hollow quaternized chitosan microspheres increase the therapeutic effect of orally administered insulin, Acta Biomater., 6, 205, 10.1016/j.actbio.2009.06.005 · DOI
  29. Pade, 1997, Estimation of the relative contribution of the transcellular and paracellular pathway to the transport of passively absorbed drugs in the Caco-2 cell culture model, Pharm. Res., 14, 1210, 10.1023/A:1012111008617 · DOI
  30. Serra, 2009, Engineering design and molecular dynamics of mucoadhesive drug delivery systems as targeting agents, Eur. J. Pharm. Biopharm., 71, 519, 10.1016/j.ejpb.2008.09.022 · DOI
  31. Kawashima, 2000, Mucoadhesive DL-lactide/glycolide copolymer nanospheres coated with chitosan to improve oral delivery of elcatonin, Pharm. Dev. Technol., 5, 77, 10.1081/PDT-100100522 · DOI
  32. Frohlich, 2012, The role of surface charge in cellular uptake and cytotoxicity of medical nanoparticles, Int. J. Nanomedicine, 7, 5577, 10.2147/IJN.S36111 · DOI
  33. Vaezifar, 2015, Characterization of PLGA/chitosan electrospun nano-biocomposite fabricated by two different methods, Int. J. Polym. Mater., 64, 64, 10.1080/00914037.2014.886244 · DOI
  34. Fang, 2017, Internalization properties of the anti-tumor α-lactalbumin-oleic acid complex, Int. J. Biol. Macromol., 96, 44, 10.1016/j.ijbiomac.2016.12.031 · DOI

Cited by

Sorted by citations

See all 86

Metadata

Authors
Dou, T., Wang, J., Han, C., Shao, X., Zhang, J., & Lu, W.
Venue
International Journal of Biological Macromolecules
Publisher
Elsevier BV
Published
2019-10
Volume
138
Pages
791-799
ISSN
0141-8130
DOI
10.1016/j.ijbiomac.2019.07.168
Cited by
86
References
1
Language
en
Licence
Funders
National Natural Science Foundation of China (81302737, 81673397)

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

How to cite

Cite CoChat for the checks on this page, and the paper for its own findings.

This page
CoChat. (2026). Citation check: Cellular uptake and transport characteristics of chitosan modified nanoparticles in Caco-2 cell monolayers. https://app.cochat.ai/papers/10.1016/j.ijbiomac.2019.07.168
The paper
Dou, T., Wang, J., Han, C., Shao, X., Zhang, J., & Lu, W. (2019). Cellular uptake and transport characteristics of chitosan modified nanoparticles in Caco-2 cell monolayers. International Journal of Biological Macromolecules. https://doi.org/10.1016/j.ijbiomac.2019.07.168