TEMPO-oxidized cellulose nanofibril film from nano-structured bacterial cellulose derived from the recently developed thermotolerant Komagataeibacter xylinus C30 and Komagataeibacter oboediens R37–9 strains
Abstract
Bacterial cellulose (BC), prepared from two recently developed thermotolerant bacterial strains (Komagataeibacter xylinus C30 and Komagataeibacter oboediens R37-9), were used as a raw material to synthesize nanofibril films. Field-emission scanning electron microscope (FE-SEM) observations confirmed the ultrafine nano-structure of BC pellicle (BCP) with average fibril widths between 50 and 60 nm. The BC was directly oxidized in a TEMPO/NaBr/NaClO system at pH of 10 for 2 h. TEMPO-oxidized bacterial cellulose nanofibrils (TOBCN) were obtained by a mild mechanical treatment and the TOBCN films were prepared through heat-drying. The oxidation yielded a recovery ratio between 70 and 80% by weight with an increase in the carboxylate content of 0.9-1.0 mmol g -1. Nanofibrillation yields were more than 90% and the resulting high aspect ratio TOBCNs were ~6 nm in average width with >800 nm in lengths, when observed under transmission electron microscope (TEM). TOBCN film of K. xylinus C30 exhibited high transparency (79%), tensile strength (142 MPa), Young's modulus (7.13 GPa), elongation around failure (3.89%), and work of fracture (2.29 MJ m-3), when compared to the TOBCN films of K. oboediens R37-9 at 23 °C and 50% RH. Coefficients of thermal expansion of both the TOBCN films were low at around 6 ppm K-1.
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CNFs are extracted through mechanical fibrillation methods including high-pressure homogenization and grinding, often aided by chemical or enzymatic pre-treatments such as TEMPO-mediated oxidation to enhance fibrillation efficiency and reduce energy demand.
“CNFs (Figure 3b), on the other hand, are extracted through mechanical fibrillation methods including high-pressure homogenization and grinding, often aided by chemical or enzymatic pre-treatments such as 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO)-mediated oxidation to enhance fibrillation efficiency and reduce energy demand [58,59,60]”
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“The characterization of NC has been reported in the literature, with various studies highlighting how morphological, structural, and thermal properties influence their performance in polymer nanocomposites [58,59,61].”
- Authors
- Chitbanyong, K., Pisutpiched, S., Khantayanuwong, S., Theeragool, G., & Puangsin, B.
- Affiliations
- Kasetsart University (TH)
- Corresponding author
- Puangsin, Buapan
- ORCID
- Venue
- International Journal of Biological Macromolecules
- Publisher
- Elsevier BV
- Published
- 2020-11
- Volume
- 163
- Pages
- 1908-1914
- ISSN
- 0141-8130 (ISSN-L)
- OpenAlex
- W3087849950
- Cited by
- 22
- References
- 50
- Citations by year
- 2026: 6 · 2025: 3 · 2024: 1 · 2023: 2 · 2022: 8 · 2021: 2
- FWCI
- 0.78
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- International Journal of Biological Macromolecules (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Research Council of Thailand
- Biodiversity-Based Economy Development Office
- Topics
- Physical Sciences › Materials Science › Biomaterials › Advanced Cellulose Research Studies
- Physical Sciences › Materials Science › Biomaterials › Nanocomposite Films for Food Packaging
- Physical Sciences › Engineering › Building and Construction › Dyeing and Modifying Textile Fibers
- Keywords
- bacterial cellulose, TEMPO-oxidized cellulose nanofibers, cellulose nanofibril films, Acetobacter xylinum, carboxylated cellulose, nanofibrillation, mechanical properties, thermal expansion, transparency, cellulose recovery
- MeSH terms
- Cellulose, Cellulose, Oxidized, Cyclic N-Oxides, Oxidation-Reduction, Tensile Strength, Acetobacteraceae, Nanostructures, Nanofibers
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