Structural framework for the understanding spectroscopic and functional signatures of the cyanobacterial Orange Carotenoid Protein families
Open accessPublished version of a preprint
Abstract
The Orange Carotenoid Protein (OCP) is a unique photoreceptor crucial for cyanobacterial photoprotection. Best studied Synechocystis sp. PCC 6803 OCP belongs to the large OCP1 family. Downregulated by the Fluorescence Recovery Protein (FRP) in low-light, high-light-activated OCP1 binds to the phycobilisomes and performs non-photochemical quenching. Recently discovered families OCP2 and OCP3 remain structurally and functionally underexplored, and no systematic comparative studies have ever been conducted. Here we present two first crystal structures of OCP2 from morphoecophysiologically different cyanobacteria and provide their comprehensive structural, spectroscopic and functional comparison with OCP1, the recently described OCP3 and all-OCP ancestor. Structures enable correlation of spectroscopic signatures with the effective number of hydrogen and discovered here chalcogen bonds anchoring the ketocarotenoid in OCP, as well as with the rotation of the echinenone's β-ionone ring in the CTD. Structural data also helped rationalize the observed differences in OCP/FRP and OCP/phycobilisome functional interactions. These data are expected to foster OCP research and applications in optogenetics, targeted carotenoid delivery and cyanobacterial biomass engineering.
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Highly accurate protein structure prediction with AlphaFold
Jumper, J., Evans, R., Pritzel, A., Green, T., Figurnov, M., Ronneberger, O., Tunyasuvunakool, K., Bates, R., Žídek, A., Potapenko, A., et al. (2021) · Nature · doi:10.1038/s41586-021-03819-2 · Cited by 43,764 · see citing works
NFκB selectivity of estrogen receptor ligands revealed by comparative crystallographic analyses
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- Authors
- Sluchanko, N. N., Maksimov, E. G., Slonimskiy, Y. B., Varfolomeeva, L. A., Bukhanko, A. Y., Egorkin, N. A., Tsoraev, G. V., Khrenova, M. G., Ge, B., Qin, S., Boyko, K. M., & Popov, V. O.
- Affiliations
- Russian Academy of Sciences (RU)
- Lomonosov Moscow State University (RU)
- China University of Petroleum, East China (CN)
- Chinese Academy of Sciences (CN)
- Corresponding author
- Sluchanko, Nikolai N.
- Qin, Song
- ORCID
- Venue
- International Journal of Biological Macromolecules
- Publisher
- Elsevier BV
- Published
- 2024-01
- Volume
- 254
- Issue
- Pt 2
- Pages
- 127874
- ISSN
- 0141-8130 (ISSN-L)
- OpenAlex
- W4388440528
- Cited by
- 18
- References
- 2
- Citations by year
- 2026: 4 · 2025: 6 · 2024: 7 · 2023: 1
- FWCI
- 1.55
- Type
- journal article
- Language
- en
- Open access
- green
- doi.org
- Licence
- Locations
- International Journal of Biological Macromolecules (publishedVersion)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
- PubMed (publishedVersion)
- Knowledge Repository of Yantai Institute of Coastal Zone Research, CAS (Yantai Institute of Coastal Zone Research) (submittedVersion)
- Funders
- Russian Science Foundation (21-44-00005)
- Moscow State University of Geodesy and Cartography
- Russian Academy of Sciences
- Ministry of Education and Science of the Russian Federation
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Photoreceptor and optogenetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
- Keywords
- orange carotenoid protein, cyanobacteria, crystal structure, phycobilisomes, non-photochemical quenching, photoprotection, ketocarotenoids, chalcogen bonding, echinenone, carotenoid biosynthesis, optogenetics, carotenoid delivery
- MeSH terms
- Bacterial Proteins, Carotenoids, Spectrum Analysis, Phycobilisomes, Synechocystis
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