Efficacy and putative mode of action of native and commercial antagonistic yeasts against postharvest pathogens of pear
Abstract
Putative mechanisms of action associated with the biocontrol capacity of four yeast strains (Cryptoccocus albidus NPCC 1248, Pichia membranifaciens NPCC 1250, Cryptoccocus victoriae NPCC 1263 and NPCC 1259) against Penicillium expansum and Botrytis cinerea were studied by means of in vitro and in situ assays. C. albidus(YP), a commercial yeast was also evaluated for comparative purposes. The yeast strains exhibited a variety of different mechanisms including: wound colonization, germination inhibition, biofilm formation, secretion of killer toxins, competition for nutrient and secretion of hydrolytic enzymes (protease, chitinase and glucanase). The relationship between strains (and their associated antagonist mechanisms) and in situ antagonist activity was also evaluated. Results indicate that mechanisms such as production of hydrolytic enzymes, the ability for colonization of wounds, production of killer toxin and inhibition of germination are the most important for biocontrol activity. Our study indicate that multiple modes of action may explain why P. membranifaciens NPCC 1250 and C. victoriae NPCC 1263 provided excellent control of postharvest pears disease.
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C. albidus produces unidentified volatile compounds that inhibit fungal growth and can display killer activity against C. glabrata.
“It also produces unidentified volatile compounds that inhibit fungal growth and can display killer activity against C. glabrata (Lutz et al. 2013).”
From Biocontrol yeasts: mechanisms and applications (2019) · cites this paper as Lutz et al. 2013
The addition of glucose or NH4NO3 to the medium reduces the biocontrol ability of C. albidus against P. expansum, but not against B. cinerea.
“The addition of glucose or NH4NO3 to the medium reduces biocontrol ability against P. expansum, but not against B. cinerea (Lutz et al. 2013).”
From Biocontrol yeasts: mechanisms and applications (2019) · cites this paper as Lutz et al. 2013
C. albidus exhibits glucanase, chitinase and protease activity in the corresponding substrate media.
“C. albidus exhibits glucanase, chitinase and protease activity in the corresponding substrate media.”
From Biocontrol yeasts: mechanisms and applications (2019) · cites this paper as Lutz et al. 2013
Cites
Wisniewski, M., Biles, C., Droby, S., McLaughlin, R., Wilson, C., & Chalutz, E. (1991) · Physiological and Molecular Plant Pathology · doi:10.1016/0885-5765(91)90033-e · Cited by 274 · see citing works
Postharvest biocontrol: new concepts and applications.
Wisniewski, M., Wilson, C., Droby, S., Chalutz, E., El-Ghaouth, A., & Stevens, C. (2007) · Biological control: a global perspective · doi:10.1079/9781845932657.0262 · Cited by 81 · see citing works
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Spadaro, D., & Droby, S. (2016) · Trends in Food Science & Technology · doi:10.1016/j.tifs.2015.11.003 · Cited by 732 · see citing works
Biocontrol yeasts: mechanisms and applications
Freimoser, F. M., Rueda-Mejia, M. P., Tilocca, B., & Migheli, Q. (2019) · World Journal of Microbiology and Biotechnology · doi:10.1007/s11274-019-2728-4 · Cited by 462 · see citing works
Parafati, L., Vitale, A., Restuccia, C., & Cirvilleri, G. (2015) · Food Microbiology · doi:10.1016/j.fm.2014.11.013 · Cited by 391 · see citing works
The Biology of Pichia membranifaciens Killer Toxins
Belda, I., Ruiz, J., Alonso, A., Marquina, D., & Santos, A. (2017) · Toxins · doi:10.3390/toxins9040112 · Cited by 111 · see citing works
Lopes, M. R., Klein, M. N., Ferraz, L. P., da Silva, A. C., & Kupper, K. C. (2015) · Microbiological Research · doi:10.1016/j.micres.2015.04.003 · Cited by 86 · see citing works
- Authors
- Lutz, M. C., Lopes, C. A., Rodriguez, M. E., Sosa, M. C., & Sangorrín, M. P.
- Affiliations
- Consejo Nacional de Investigaciones Científicas y Técnicas (AR)
- Universidad Nacional del Comahue (AR)
- Corresponding author
- Sangorrín, Marcela P.
- Venue
- International Journal of Food Microbiology
- Publisher
- Elsevier BV
- Published
- 2013-06
- Volume
- 164
- Issue
- 2-3
- Pages
- 166-172
- ISSN
- 0168-1605 (ISSN-L)
- OpenAlex
- W2041232781
- Cited by
- 110
- References
- 2
- Citations by year
- 2026: 5 · 2025: 5 · 2024: 10 · 2023: 12 · 2022: 11 · 2021: 11 · 2020: 8 · 2019: 8 · 2018: 10 · 2017: 11 · 2016: 8 · 2015: 6 · 2014: 2 · 2013: 3
- FWCI
- 7.65
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- International Journal of Food Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Consejo Nacional de Investigaciones Científicas y Técnicas
- Agencia Nacional de Promoción Científica y Tecnológica (CONICET)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Postharvest Quality and Shelf Life Management
- Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Fungal Plant Pathogen Control
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Yeasts and Rust Fungi Studies
- Keywords
- antagonistic yeasts, biological control, Penicillium expansum, Botrytis cinerea, Cryptococcus albidus, Pichia membranifaciens, mechanism of action, wound colonization, germination inhibition, biofilm formation, killer toxin, hydrolytic enzymes, chitinase, glucanase, proteolytic enzymes
- MeSH terms
- Antibiosis, Cryptococcus, Fruit, Hydrolases, Penicillium, Pichia, Plant Diseases, Yeasts, Colony Count, Microbial, Botrytis, Pyrus, Killer Factors, Yeast, Biological Control Agents
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