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Cited by 16Antibiotic Resistance in BacteriaFood Safety and HygieneSalmonella and Campylobacter epidemiology

Microbiological quality and antimicrobial resistance of Bacteria species recovered from ready-to-eat food, water samples, and palm swabs of food vendors in Accra, Ghana

Open access
Dela, Helena, Egyir, Beverly, Behene, Eric, Sulemana, Hamdiya, Tagoe, Rodalyn, Bentil, Ronald, Bongo, Richard N.A., Bonfoh, Bassirou +3 more·2023·International Journal of Food Microbiology
DOI 10.1016/j.ijfoodmicro.2023.110195
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Abstract

This study sought to investigate microbial quality and antimicrobial resistance of bacteria species from Ready-to-Eat (RTE) food, water, and vendor palm swab samples. Between 2019 and 2020, RTE food, water and vendor palm swab samples were collected from food vending sites in Accra, Ghana. Samples were cultured and confirmed using the Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF). Antimicrobial susceptibility testing (AST) was conducted using disk diffusion method. Beta-lactamase and Diarrheagenic Escherichia coli (DEC) genes were determined using Polymerase Chain Reaction (PCR). Total plate count (TPC) and Total coliform count (TCC) were performed on food and water samples. In total, 179 RTE food, 72 water and 10 vendor palm swab samples were collected. Enterobacter spp. (16.8 %), Citrobacter spp. (10.1 %), Enterococcus faecalis (7.8 %), Pseudomonas spp. (6.7 %) and Klebsiella pneumoniae (4.0 %) occurred in food. Isolates from water and palm were Klebsiella pneumoniae (20.8 %), Aeromonas spp. (16.7 %) and Enterobacter cloacae (11.1 %). Resistance to Amoxicillin-clavulanate, Tetracycline, Azithromycin, Sulfamethoxazole-trimethoprim, and Nitrofurantoin were common among Enterobacterales. High mean TPC and TCC showed in some RTE food and different water types used in vending depicting their unsafe condition for consumption and usage. The blaSHV and blaTEM genes were present in some Enterobacterales from food and water. The lt gene was identified in two food samples. AMR organisms associated with nosocomial infections in the samples investigated, calls for continuous surveillance in the food industry in Ghana. Also, the unsafe outcome of RTE food and water depicts the need for the enforcement of Ghana's food safety laws.

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Research works printed but not linked: 4

  1. Addo, 2007, [PDF] Food and its preparation conditions in hotels in Accra, Ghana: a concern for food safety | Semantic Scholar, Afr. J. Food Agric. Nutr. Dev., 7
  2. Feglo, 2012, (PDF) Bacterial contamination of street vending food in Kumasi, Ghana, J. Med. Biomed. Sci., 1, 1
  3. Feng, 2020, BAM Chapter 4: enumeration of Escherichia coli and the coliform bacteria | FDA
  4. Newman, 2015, 09, 1

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Authors
Dela, H., Egyir, B., Behene, E., Sulemana, H., Tagoe, R., Bentil, R., Bongo, R. N. A., Bonfoh, B., Zinsstag, J., Bimi, L., & Addo, K. K.
Affiliations
  • University of Ghana (GH)
  • Swiss Centre for Scientific Research (CI)
  • Swiss Tropical and Public Health Institute (CH)
Corresponding author
Dela, Helena
ORCID
Venue
International Journal of Food Microbiology
Publisher
Elsevier BV
Published
2023-07
Volume
396
Pages
110195
ISSN
0168-1605 (ISSN-L)
DOI
10.1016/j.ijfoodmicro.2023.110195
OpenAlex
W4362560703
Cited by
16
References
49
Citations by year
2026: 5 · 2025: 5 · 2024: 6
FWCI
1.14
Type
journal article
Language
en
Open access
Licence
Locations
  • International Journal of Food Microbiology (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • edoc (University of Basel) (submittedVersion)
Funders
  • African Academy of Sciences (DEL-15-008)
  • Wellcome Trust (107753/A/15/Z)
  • Government of the United Kingdom
  • New Partnership for Africa's Development
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Antibiotic Resistance in Bacteria
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Food Safety and Hygiene
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Salmonella and Campylobacter epidemiology
Keywords
ready-to-eat foods, food vendors, antimicrobial resistance, foodborne bacteria, water quality, total plate count, total coliform count, Enterobacterales, Klebsiella pneumoniae, Aeromonas, Enterobacter cloacae, Enterococcus faecalis, Pseudomonas, beta-lactamase genes, diarrheagenic Escherichia coli, food safety, nosocomial infections, Accra
MeSH terms
Anti-Bacterial Agents, Bacteria, beta-Lactamases, Escherichia coli, Food Microbiology, Ghana, Microbial Sensitivity Tests, Drug Resistance, Bacterial

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