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Reversion of multidrug resistance by co-encapsulation of doxorubicin and curcumin in chitosan/poly(butyl cyanoacrylate) nanoparticles
Abstract
Co-encapsulated doxorubicin (DOX) and curcumin (CUR) in poly(butyl cyanoacrylate) nanoparticles (PBCA-NPs) were prepared with emulsion polymerization and interfacial polymerization. The mean particle size and mean zeta potential of CUR-DOX-PBCA-NPs were 133 ± 5.34 nm in diameter and +32.23 ± 4.56 mV, respectively. The entrapment efficiencies of doxorubicin and curcumin were 49.98 ± 3.32% and 94.52 ± 3.14%, respectively. Anticancer activities and reversal efficacy of the formulations and various combination approaches were assessed using 3-[4,5-dimethylthiazol-2-yl] 2,5-diphenyltetrazolium bromide assay and western blotting. The results showed that the dual-agent loaded PBCA-NPs system had the similar cytotoxicity to co-administration of two single-agent loaded PBCA-NPs (DOX-PBCA-NPs+CUR-PBCA-NPs), which was slightly higher than that of the free drug combination (DOX+CUR) and one free drug/another agent loaded PBCA-NPs combination (DOX+CUR-PBCA-NPs or CUR+DOX-PBCA-NPs). The simultaneous administration of doxorubicin and curcumin achieved the highest reversal efficacy and down-regulation of P-glycoprotein in MCF-7/ADR cell lines, an MCF-7 breast carcer cell line resistant to adriamycin. Multidrug resistance can be enhanced by combination delivery of encapsulated cytotoxic drugs and reversal agents.
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“Especially, anticancer drugs encapsulated in polymeric micelles have shown a better accumulation in the targeted site, thus reducing side effects on noncancerous tissues [46,47,48].”
From Recent Advances in Nanomicelles Delivery Systems. (2020) · cites this paper as [46]
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Metadata
- Authors
- Duan, J., Mansour, H. M., Zhang, Y., Deng, X., Chen, Y., Wang, J., Pan, Y., & Zhao, J.
- Affiliations
- Central South University (CN)
- University of Kentucky (US)
- Emory University (US)
- Corresponding author
- Zhao, Jinfeng
- Venue
- International Journal of Pharmaceutics
- Publisher
- Elsevier BV
- Published
- 2012-04
- Volume
- 426
- Issue
- 1-2
- Pages
- 193-201
- ISSN
- 0378-5173 (ISSN-L)
- OpenAlex
- W2005475049
- Cited by
- 194
- References
- 38
- Citations by year
- 2026: 3 · 2025: 9 · 2024: 11 · 2023: 8 · 2022: 8 · 2021: 9 · 2020: 19 · 2019: 22 · 2018: 18 · 2017: 18 · 2016: 17 · 2015: 19 · 2014: 16 · 2013: 12 · 2012: 5
- FWCI
- 6.74
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- International Journal of Pharmaceutics (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Lotus Scholars Program (200734)
- Topics
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advanced Drug Delivery Systems
- Keywords
- multidrug resistance reversal, doxorubicin, curcumin, chitosan, co-encapsulation, MCF-7/ADR cells, breast cancer, anticancer drug delivery, combination chemotherapy
- MeSH terms
- Antineoplastic Combined Chemotherapy Protocols, Breast Neoplasms, Calorimetry, Differential Scanning, Chemistry, Pharmaceutical, Chromatography, Gel, Curcumin, Doxorubicin, Drug Carriers, Drug Combinations, Drug Compounding, Enbucrilate, Female, Humans, Particle Size, Technology, Pharmaceutical, Down-Regulation, Spectroscopy, Fourier Transform Infrared, Drug Resistance, Multiple, ATP Binding Cassette Transporter, Subfamily B, Drug Resistance, Neoplasm, ATP Binding Cassette Transporter, Subfamily B, Member 1, Nanotechnology, Cell Line, Tumor, Chitosan, Nanoparticles
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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