Regulation of immunophenotype modulation of monocytes-macrophages from M1 into M2 by prostate cancer cell-culture supernatant via transcription factor STAT3
Abstract
Background Transcription factor STAT3 has a prominent innate immunity effect on cancer progression. We determined the regulation of STAT3 in the immunophenotype modulation of macrophages from M1 into M2 induced by the cell-culture supernatant of the Prostate-Cancer line PC3. Methods Monocytes-macrophages from healthy donors were cultured in the supernatant of PC3 cells, membrane proteins, and intracytoplasmic and phosphorylated STAT3 were measured using flow cytometry, while cytokines and growth factors were studied using luminescence. Cytotoxicity and nitric oxide were evaluated via colorimetric assays. Results The supernatant of PC3 prostate-tumor cells effectively induced macrophages toward an M2 profile, and the expression of phosphorylated STAT3 in the monocytes-macrophages notably increased, and mainly related to IL-10. In the group of monocytes-macrophages treated with a STAT3 inhibitor, the macrophages were induced toward an M1 phenotype. Conclusions In this study, we showed that the secretion profile of PC3 prostate-cancer cells induces a change in macrophage phenotype from M1 into M2, and that the phenomenon is related to phosphorylation of transcription factor STAT3 and IL-10.
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IL-6 and IL-10 are abundant in tumor microenvironments originated from myeloid cells, fibroblasts and tumor cells, and they further induce M2 macrophage differentiation through STAT3.
“IL-6 and IL-10 are abundant in tumor microenvironments originated from myeloid cells, fibroblasts and tumor cells, and they further induce M2 macrophage differentiation through STAT3 (215, 216).”
From IL-6 in inflammation, autoimmunity and cancer (2021) · cites this paper as [215]
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- Authors
- Solís-Martínez, R., Cancino-Marentes, M., Hernández-Flores, G., Ortiz-Lazareno, P., Mandujano-Álvarez, G., Cruz-Gálvez, C., Sierra-Díaz, E., Rodríguez-Padilla, C., Jave-Suárez, L. F., Aguilar-Lemarroy, A., & Bravo-Cuellar, A.
- Affiliations
- Universidad de Guadalajara (MX)
- Universidad Juárez Autónoma de Tabasco (MX)
- Venue
- Immunology Letters
- Publisher
- Elsevier BV
- Published
- 2018-04
- Volume
- 196
- Pages
- 140-148
- ISSN
- 0165-2478 (ISSN-L)
- OpenAlex
- W2794377359
- Cited by
- 47
- References
- 46
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 5 · 2023: 9 · 2022: 11 · 2021: 6 · 2020: 5 · 2019: 4 · 2018: 2
- FWCI
- 1.82
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Immunology Letters (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Immune cells in cancer
- Health Sciences › Medicine › Oncology › Cytokine Signaling Pathways and Interactions
- Health Sciences › Medicine › Psychiatry and Mental health › Cancer, Stress, Anesthesia, and Immune Response
- Keywords
- STAT3, M2 macrophages, M1 macrophages, macrophage polarization, prostate cancer, PC-3 cells, tumor cell supernatant, IL-10, STAT3 phosphorylation, monocytes, STAT3 inhibitor, nitric oxide, cytokines
- MeSH terms
- PC-3 Cells, Cells, Cultured, Humans, Macrophages, Male, Monocytes, Phosphorylation, Prostatic Neoplasms, Immunophenotyping, Interleukin-10, Culture Media, Conditioned, STAT3 Transcription Factor
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