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Cited by 105T-cell and B-cell ImmunologyProtein Kinase Regulation and GTPase SignalingCellular transport and secretion

The Rap GTPases Regulate B Cell Morphology, Immune-Synapse Formation, and Signaling by Particulate B Cell Receptor Ligands

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Lin, Kevin B.L., Freeman, Spencer A., Zabetian, Saba, Brugger, Hayley, Weber, Michele, Lei, Victor, Dang-Lawson, May, Tse, Kathy W.K. +3 more·2008·Immunity
DOI 10.1016/j.immuni.2007.11.019
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Abstract

B lymphocytes spread and extend membrane processes when searching for antigens and form immune synapses upon contacting cells that display antigens on their surface. Although these dynamic morphological changes facilitate B cell activation, the signaling pathways underlying these processes are not fully understood. We found that activation of the Rap GTPases was essential for these changes in B cell morphology. Rap activation was important for B cell receptor (BCR)- and lymphocyte-function-associated antigen-1 (LFA-1)-induced spreading, for BCR-induced immune-synapse formation, and for particulate BCR ligands to induce localized F-actin assembly and membrane-process extension. Rap activation and F-actin assembly were also required for optimal BCR signaling in response to particulate antigens but not soluble antigens. Thus by controlling B cell morphology and cytoskeletal organization, Rap might play a key role in the activation of B cells by particulate and cell-associated antigens.

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  • Similar defects in B cell immune synapses likely underlie poor antibody responses after vaccination or infection in HEM1-deficient patients.

    “Similar defects in B cell immune synapses likely underlie poor antibody responses after vaccination or infection [9, 38].”

    From HEM1 Actin Immunodysregulatory Disorder: Genotypes, Phenotypes, and Future Directions (2022) · cites this paper as [38]

    The Results (page unavailable) show that blocking Rap activation impaired immune-synapse formation in experimental B cells. The paper does not study HEM1-deficient patients or establish that synapse defects underlie their poor antibody responses after vaccination or infection. (LLM, conf=0.87)

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Cited by

Sorted by citations

Authors
Lin, K. B. L., Freeman, S. A., Zabetian, S., Brugger, H., Weber, M., Lei, V., Dang-Lawson, M., Tse, K. W. K., Santamaria, R., Batista, F. D., & Gold, M. R.
Affiliations
  • University of British Columbia (CA)
  • Cancer Research UK (GB)
Corresponding author
Gold, Michael R.
ORCID
Venue
Immunity
Publisher
Elsevier BV
Published
2008-01
Volume
28
Issue
1
Pages
75-87
ISSN
1074-7613 (ISSN-L)
DOI
10.1016/j.immuni.2007.11.019
OpenAlex
W2086285294
Cited by
105
References
0
Citations by year
2025: 4 · 2024: 1 · 2023: 1 · 2022: 4 · 2021: 1 · 2020: 4 · 2019: 5 · 2018: 10 · 2017: 5 · 2016: 2 · 2015: 7 · 2014: 3 · 2013: 6 · 2012: 5
FWCI
6.17
Type
journal article
Language
en
Open access
Licence
Locations
  • Immunity (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Michael Smith Health Research BC
  • Canadian Institutes of Health Research
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
Keywords
Rap GTPases, immune synapse formation, B cell receptor signaling, particulate antigen, LFA-1, F-actin assembly, cytoskeleton, B cell activation
MeSH terms
Actins, Animals, B-Lymphocytes, Cell Line, Enzyme Activation, Humans, Lymphocyte Activation, Microscopy, Electron, Scanning, Receptors, Antigen, B-Cell, Signal Transduction, Antigen Presentation, Microscopy, Confocal, rap GTP-Binding Proteins, Mice

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