Cited by 17Extracellular vesicles in diseaseLiver physiology and pathologyMesenchymal stem cell research

Exploring the cytoprotective role of mesenchymal stem Cell-Derived exosomes in chronic liver Fibrosis: Insights into the Nrf2/Keap1/p62 signaling pathway

Open access
Al Saihati, Hajir A., Badr, Omnia A., Dessouky, Arigue A., Mostafa, Ola, Samir Farid, Ayman, Aborayah, Nashwa H, Abdullah Aljasir, Mohammad, Baioumy, Bodour +8 more·2024·International Immunopharmacology

Abstract

Hepatic fibrosis is a common pathology present in most chronic liver diseases. Autophagy is a lysosome-mediated intracellular catabolic and recycling process that plays an essential role in maintaining normal hepatic functions. Nuclear factor erythroid 2-like 2 (Nrf2) is a transcription factor responsible for the regulation of cellular anti-oxidative stress response. This study was designed to assess the cytoprotective effect of mesenchymal stem cell-derived exosomes (MSC-exos) on endothelial-mesenchymal transition (EMT) in Carbon Tetrachloride (CCL4) induced liver fibrosis. Rats were treated with 0.1 ml of CCL4 twice weekly for 8 weeks, followed by administration of a single dose of MSC-exos. Rats were then sacrificed after 4 weeks, and liver samples were collected for gene expression analyses, Western blot, histological studies, immunohistochemistry, and transmission electron microscopy. Our results showed that MSC-exos administration decreased collagen deposition, apoptosis, and inflammation. Exosomes modulate the Nrf2/Keap1/p62 pathway, restoring autophagy and Nrf2 levels through modulation of the non-canonical pathway of Nrf2/Keap1/p62. Additionally, MSC-exos regulated miR-153-3p, miR-27a, miR-144 and miRNA-34a expression. In conclusion, the present study shed light on MSC-exos as a cytoprotective agent against EMT and tumorigenesis in chronic liver inflammation.

Integrity signals

Registry

Crossref · 10.1016/j.intimp.2024.112934

Cites

98 of 98 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

1 retracted

Flagged first, then by citations

2 of the 100 references couldn’t be matched to a known paper.

Cited by

Sorted by citations

Authors
Al Saihati, H. A., Badr, O. A., Dessouky, A. A., Mostafa, O., Samir Farid, A., Aborayah, N. H., Abdullah Aljasir, M., Baioumy, B., Mahmoud Taha, N., El-Sherbiny, M., Hamed Al‑Serwi, R., Ramadan, M. M., Salim, R. F., Shaheen, D., E.M Ali, F., & Ebrahim, N.
Affiliations
  • Benha University (EG)
  • Zagazig University (EG)
  • Mansoura University (EG)
Corresponding author
Ebrahim, Nesrine
Venue
International Immunopharmacology
Publisher
Elsevier BV
Published
2024-11
Volume
141
Pages
112934
ISSN
1567-5769 (ISSN-L)
OpenAlex
W4401813474
Cited by
17
References
100
Citations by year
2026: 5 · 2025: 10 · 2024: 2
FWCI
2.32
Type
journal article
Language
en
Open access
Locations
  • International Immunopharmacology (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
Funders
  • Princess Nourah Bint Abdulrahman University (PNURSP2024R199, MHIRSP2024006)
  • AlMaarefa University
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
  • Health Sciences › Medicine › Hepatology › Liver physiology and pathology
  • Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
Keywords
mesenchymal stem cell-derived exosomes, chronic liver fibrosis, autophagy, endothelial-to-mesenchymal transition, carbon tetrachloride-induced liver injury, miR-153-3p, miR-27a, miR-144, miR-34a, cytoprotection, oxidative stress, collagen deposition, apoptosis, liver inflammation
MeSH terms
Sequestosome-1 Protein, Kelch-Like ECH-Associated Protein 1, Animals, Autophagy, Carbon Tetrachloride, Liver, Liver Cirrhosis, Male, Signal Transduction, Rats, Sprague-Dawley, MicroRNAs, NF-E2-Related Factor 2, Rats, Exosomes, Mesenchymal Stem Cells

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review