Multi-platform-based characterization of ferroptosis in human colorectal cancer
Open accessAbstract
Ferroptosis is a type of programmed cell death potentially playing an important role in colorectal cancer (CRC) development. However, comprehensive investigations toward ferroptosis in human CRC are lacking. Here, we performed multiple investigations on cancer and para-cancer tissues. We demonstrated that the changes of structural variation and chromatin accessibility in CRC were more associated with the altered mRNA expression of ferroptosis-related genes (FRGs), and the expression of CDKN2A , GPX4 , ALOXE3 , and LINC00336 was related to the overall survival rates. Subsequently, we revealed that CYBB and YAP1 were potentially the hub genes, and that HSF1 and STAT2 were potentially FRGs' upstream transcription factors. Finally, we depicted the crosstalk between ferroptosis and necrosis, autophagy, and apoptosis. Based on multi-dimensional analyses, we characterized ferroptosis, probable core genes, and the upstream regulators in human CRC. The findings here may improve our understanding of ferroptosis in CRC and provide new opportunities for clinical diagnosis and treatment.
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Apoptosis-related genes ATF4, JUN, GADD45G, ITGB1, RPS3, and ANP32A are significantly up-regulated in neurons within AD monocyte-cocultured hCO-MPSs.
“The expression of cell apoptosis-related genes ATF4, JUN, GADD45G, ITGB1, RPS3, and ANP32A (65) was also significantly up-regulated in neurons within AD monocyte-cocultured hCO-MPSs (Fig. 5D), supporting the in vitro observations.”
From Understanding monocyte-driven neuroinflammation in Alzheimer’s disease using human cortical organoid microphysiological systems (2025) · cites this paper as [65]
Citation (65) is attached to the description of the six genes as apoptosis-related; the up-regulation in AD monocyte-cocultured neurons is presented as the citing authors’ own Figure 5D result. The cited paper’s Results section on crosstalk discusses apoptosis-related genes in colorectal cancer, but the passages retrieved do not identify ATF4, JUN, GADD45G, ITGB1, RPS3, and ANP32A as a group of apoptosis-related genes. Searches for the gene list and for ATF4 with apoptosis did not verify that classification. (LLM, conf=0.72)
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- Authors
- Zhong, Y., Zhang, W., Yu, H., Lin, L., Gao, X., He, J., Li, D., Chen, Y., Zeng, Z., Xu, Y., Tang, D., & Dai, Y.
- Affiliations
- Jinan University (CN)
- Shenzhen University (CN)
- Corresponding author
- Tang, Donge
- Dai, Yong
- ORCID
- Venue
- iScience
- Publisher
- Elsevier BV
- Published
- 2022-08
- Volume
- 25
- Issue
- 8
- Pages
- 104750
- ISSN
- 2589-0042 (ISSN-L)
- PMC
- PMC9356096
- OpenAlex
- W4285499763
- Cited by
- 14
- References
- 36
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 3 · 2023: 4 · 2022: 3
- FWCI
- 1.94
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- iScience (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Natural Science Foundation of China (82003172)
- China Postdoctoral Science Foundation
- Basic and Applied Basic Research Foundation of Guangdong Province (2019A1515111138)
- Topics
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Ferroptosis and cancer prognosis
- Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
- Keywords
- ferroptosis, colorectal cancer, ferroptosis-related genes, structural variants, chromatin accessibility, CDKN2A, GPX4, ALOXE3, CYBB, YAP, HSF1, STAT2, overall survival, hub genes
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