Bone marrow-derived myeloid cells transiently colonize the brain during postnatal development and interact with glutamatergic synapses
Open accessAbstract
Although the roles of embryonic yolk sac-derived, resident microglia in neurodevelopment were extensively studied, the possible involvement of bone marrow-derived cells remains elusive. In this work, we used a fate-mapping strategy to selectively label bone marrow-derived cells and their progeny in the brain (FLT3 + IBA1 + ). FLT3 + IBA1 + cells were confirmed to be transiently present in the healthy brain during early postnatal development. FLT3 + IBA1 + cells have a distinct morphology index at postnatal day(P)0, P7, and P14 compared with neighboring microglia. FLT3 + IBA1 + cells also express the microglial markers P2RY12 and TMEM119 and interact with VGLUT1 synapses at P14. Scanning electron microscopy indeed showed that FLT3 + cells contact and engulf pre-synaptic elements. Our findings suggest FLT3 + IBA1 + cells might assist microglia in their physiological functions in the developing brain including synaptic pruning which is performed using their purinergic sensors. Our findings stimulate further investigation on the involvement of peripheral macrophages during homeostatic and pathological development.
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Peripheral monocytes and macrophages were recently shown to infiltrate the brain during normal physiological conditions, in the first postnatal weeks, but their roles in the healthy adult brain have remained undetermined.
“Peripheral monocytes and macrophages were recently shown to infiltrate the brain during normal physiological conditions, in the first postnatal weeks, but their roles in the healthy adult brain have remained undetermined [66].”
From Sex chromosomes and sex hormones differently shape microglial properties during normal physiological conditions in the adult mouse hippocampus (2025) · cites this paper as [66]
The Abstract reports transient bone marrow-derived myeloid cells in the healthy brain during early postnatal development, supporting the first part of the claim. The Results report a lack of FLT3+ cells in adult whole-brain isolates, but the paper does not establish the broader claim that peripheral monocytes’ and macrophages’ roles in the healthy adult brain remain undetermined. (LLM, conf=0.83)
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References 86 · Cited by 5
Research works printed but not linked: 2
- [31]Ginhoux F. Greter M. Leboeuf M. Nandi S. See P. Gokhan S. Mehler M.F. Conway S.J. Ng L.G. Stanley E.R. Fate mapping analysis reveals that adult microglia derive from primitive macrophages Science 330 2010 841 845 10.1126/science.1194637 20966214 PMC3719181 · DOI · Paper page
- [74]Institute of Medicine (US) Forum on Neuroscience and Nervous System Overview of the Glutamatergic System Glutamate-Related Biomarkers in Drug Development for Disorders of the Nervous System: Workshop Summary 2011 National Academies Press (US) 21977546
- Authors
- Carrier, M., Robert, M.-È., St-Pierre, M.-K., Ibáñez, F. G., Gonçalves de Andrade, E., Laroche, A., Picard, K., Vecchiarelli, H. A., Savage, J. C., Boilard, É., Desjardins, M., & Tremblay, M.-È.
- Affiliations
- University of Victoria (CA)
- Centre de recherche du CHU de Québec-Université Laval (CA)
- Université Laval (CA)
- University of British Columbia (CA)
- Corresponding author
- Tremblay, Marie-Ève
- ORCID
- Venue
- iScience
- Publisher
- Elsevier BV
- Published
- 2024-07
- Volume
- 27
- Issue
- 7
- Pages
- 110037
- ISSN
- 2589-0042 (ISSN-L)
- PMC
- PMC11253522
- OpenAlex
- W4398177079
- Cited by
- 5
- References
- 86
- Citations by year
- 2026: 2 · 2025: 3
- FWCI
- 0.78
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- iScience (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Fonds de recherche du Québec
- Canadian Institutes of Health Research (FDN341846)
- Natural Sciences and Engineering Research Council of Canada (RGPIN-2014-05308)
- McGill University
- Canada Research Chairs
- Max Planck Instituut voor Psycholinguïstiek
- Consejo Nacional de Humanidades, Ciencias y Tecnologías
- Royal Society of Canada
- University of Victoria
- Michael Smith Health Research BC
- Topics
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- bone marrow-derived myeloid cells, postnatal brain development, microglia, peripheral macrophages, glutamatergic synapses, synaptic pruning, purinergic signaling, neuroinflammation
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